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Am J Perinatol 2022; 39(11): 1236-1240
DOI: 10.1055/s-0040-1721849
Original Article

The Acylcarnitine Profile in Dried Blood Spots is Affected by Hematocrit: A Study of Newborn Screening Samples in Very-Low-Birth-Weight Infants

Mitsuhiro Haga 
1   Department of Neonatology, Saitama Children's Medical Center, Saitama, Japan
2   Department of Pediatrics, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama, Japan
,
Mitsuhisa Isobe
3   Division of Health Science, Saitama City Institute of Health Science and Research, Saitama, Japan
,
Ken Kawabata
1   Department of Neonatology, Saitama Children's Medical Center, Saitama, Japan
,
Masaki Shimizu
1   Department of Neonatology, Saitama Children's Medical Center, Saitama, Japan
,
Hiroshi Mochizuki
4   Department of Metabolism and Endocrinology, Saitama Children's Medical Center, Saitama, Japan
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Abstract

Objective The acylcarnitine profile is analyzed in dried blood spots (DBS) to screen for inborn errors of metabolism. Hematocrit (Ht) is known to affect the result of quantitative analyses of DBS samples; however, the effects of Ht on the acylcarnitine profiles in DBS have not been studied in actual samples from newborns.

Study Design The acylcarnitine profiles in DBS for newborn screening tests and Ht levels of very-low-birth-weight infants were obtained from medical records. We investigated the relationship between Ht and each acylcarnitine using Pearson's correlation coefficient (r).

Results We examined 77 newborns in this study. There was a significantly positive correlation between Ht and C0, C2, C12, C16, C18, C18:1, and C18:1-OH, respectively (p < 0.0025). The correlation was the greatest on C2 (r = 0.59).

Conclusion This study clarifies that Ht and C0, C2, C12, C16, C18, C18:1, and C18:1-OH are significantly correlated in DBS, which is consistent with previous studies. Hence, the effect of Ht should be considered when interpreting the results of acylcarnitine profiles in DBS.

Key Points

  • Acylcarnitine profile in dried blood spots (DBS) is affected by the hematocrit (Ht) of the sample.

  • There are positive correlations between Ht and C0, C2, C12, C16, C18, and C18:1-OH in DBS.

  • We should be aware of the effects of Ht on acylcarnitine profiles in DBS.

Keywords

acylcarnitine profile - mass-spectrometry - dried blood spots - hematocrit

Supplementary Material



Publication History

Received: 29 June 2020

Accepted: 16 November 2020

Article published online:
29 December 2020

© 2020. Thieme. All rights reserved.

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