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DOI: 10.4103/1305-7456.156848
Effect of storage solutions on microhardness of crown enamel and dentin
Publication History
Publication Date:
04 September 2019 (online)
ABSTRACT
Objective: The aim of this study was to determine alterations in microhardness of crown dentin and enamel, after 2 and 12-month storage in de-ionized water, 0.2% glutaraldehyde, Hanks’ Balanced Salt Solution (HBSS), 0.1% sodium hypochlorite (NaOCl) or 0.1% thymol. Materials and Methods: Freshly extracted, nonsterile 60 intact human premolars were distributed to five groups. Six teeth from each group were evaluated after two, and other six teeth were evaluated after 12 months storage. After grinding and polishing of teeth, Vickers hardness was evaluated with making indentations on enamel and dentin, using a pyramid diamond indenter tip exerting 100 g load for 15 s. Results: After 2 months storage in solutions, range of the hardness values (HV) of enamel and dentin were in between 315–357 and 64–67, respectively. However, 12 months storage of the teeth resulted in a statistically significant decrease in microhardness when compared to microhardness of teeth stored for 2 months (P = 0.001). Although the differences were not significant regarding solutions, all solutions decreased the microhardness both in enamel and dentin (P > 0.05). However, decrease in microhardness was relatively less in de-ionized water and thymol solutions while glutaraldehyde decreased microhardness the most: 63% for enamel and 53% for dentin. Conclusions: Microhardness of enamel and dentin was in an acceptable range when teeth were stored for 2 months in de-ionized water, glutaraldehyde, HBSS, NaOCl or in thymol; thus, teeth kept up to 2 months in these solutions can be used for mechanical in vitro tests. However, 12 months storage significantly decreased the microhardness of enamel and dentin.
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Conflict of Interest
None declared
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REFERENCES
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Correspondence:
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REFERENCES
- 1 Currey JD, Brear K. Hardness, young's modulus and yield stress in mammalian mineralized tissues. J Mater Sci Mater Med 1990; 1: 14-20
- 2 Stachowiak GW, Batchelor AW, Stachowiak GB. Characterization of test specimens. In: Dowson D. editor. Experimental Methods in Tribology. 1st ed.. Oxford: Elsevier; 2004: 115-50
- 3 Habelitz S, Marshall Jr GW, Balooch M, Marshall SJ. Nanoindentation and storage of teeth. J Biomech 2002; 35: 995-8
- 4 Toparli M, Koksal NS. Hardness and yield strength of dentin from simulated nano-indentation tests. Comput Methods Programs Biomed 2005; 77: 253-7
- 5 Bedran-Russo AK, Pashley DH, Agee K, Drummond JL, Miescke KJ. Changes in stiffness of demineralized dentin following application of collagen crosslinkers. J Biomed Mater Res B Appl Biomater 2008; 86: 330-4
- 6 White SN, Paine ML, Ngan AY, Miklus VG, Luo W, Wang H. et al. Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. J Biol Chem 2007; 282: 5340-5
- 7 Kishen A, Asundi A. Experimental investigation on the role of water in the mechanical behavior of structural dentine. J Biomed Mater Res A 2005; 73: 192-200
- 8 Strawn SE, White JM, Marshall GW, Gee L, Goodis HE, Marshall SJ. Spectroscopic changes in human dentine exposed to various storage solutions – short term. J Dent 1996; 24: 417-23
- 9 Muhleman HR. Storage medium and enamel hardness. Helv Odontol Acta 1964; 8: 112-7
- 10 Tesch W, Eidelman N, Roschger P, Goldenberg F, Klaushofer K, Fratzl P. Graded microstructure and mechanical properties of human crown dentin. Calcif Tissue Int 2001; 69: 147-57
- 11 Baillif P, Chouikhi B, Barbanson L, Touray JC. Dissolution kinetics of glass fibres in saline solution: In vitro persistence of a sparingly soluble aluminium-rich leached layer. J Mater Sci 1995; 30: 5691-9
- 12 Kitasako Y, Burrow MF, Nikaido T, Tagami J. The influence of storage solution on dentin bond durability of resin cement. Dent Mater 2000; 16: 1-6
- 13 White JM, Goodis HE, Marshall SJ, Marshall GW. Sterilization of teeth by gamma radiation. J Dent Res 1994; 73: 1560-7
- 14 Craig RG, Peyton FA. The micro-hardness of enamel and dentin. J Dent Res 1958; 37: 661-8
- 15 Wemes JC, Arends J. The hardness of bovine dentine after glutaraldehyde treatment. Oral Surg Oral Med Oral Pathol 1984; 58: 722-4
- 16 Qian L, Li M, Zhou Z, Yang H, Shi X. Comparison of nano-indentation hardness to microhardness. Surf Coat Technol 2005; 195: 264-71
- 17 Bao T, Morrison PW, Woyczynski W. AFM Nanoindentation as a method to determine microhardness of hard thin films. Mater Res Soc Symp Proc 1998; 517: 395-402
- 18 He LH, Swain MV. Enamel – A functionally graded natural coating. J Dent 2009; 37: 596-603
- 19 Alrabii SA, Zumot LY. Chip thickness and microhardness prediction models during turning of medium carbon steel. J Appl Math 2007; 2: 1-12
- 20 Bozkurt FO, Tagtekin DA, Hayran O, Stookey GK, Yanikoglu FC. Accuracy of ultrasound measurement of progressive change in occlusal enamel thickness. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 99: 101-5
- 21 Ari H, Erdemir A, Belli S. Evaluation of the effect of endodontic irrigation solutions on the microhardness and the roughness of root canal dentin. J Endod 2004; 30: 792-5
- 22 Arends J, Ogaard B, Ruben J, Wemes J, Rölla G. Influence of glutardialdehyde on dentin demineralization in vitro and in vivo . Scand J Dent Res 1989; 97: 297-300
- 23 Ten CateAR. Oral Histology: Development, Structure, and Function. 4th ed.. St. Louis: Mosby; 1994: 141-90