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Thromb Haemost 2010; 104(06): 1242-1249
DOI: 10.1160/TH09-10-0744
Animal Models
Schattauer GmbH

Evaluation of AR-H067637, the active metabolite of the new direct thrombin inhibitor AZD0837, in models of venous and arterial thrombosis and bleeding in anaesthetised rats

Susanne Pehrsson
1   AstraZeneca R&D, Mölndal, Sweden
,
Karin Johansson
1   AstraZeneca R&D, Mölndal, Sweden
,
Magnus Kjaer
1   AstraZeneca R&D, Mölndal, Sweden
,
Margareta Elg
1   AstraZeneca R&D, Mölndal, Sweden
› Author Affiliations Financial support:AstraZeneca, Mölndal, Sweden, supported this study.
Further Information

Publication History

Received: 30 October 2009

Accepted after major revision: 30 July 2010

Publication Date:
24 November 2017 (online)

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Summary

AZD0837, currently in clinical development, is a once-daily oral anticoagulant that is bioconverted to AR-H067637, a selective, reversible direct thrombin inhibitor (DTI). When developing a new DTI, the anti-thrombotic effects are commonly investigated in in vivo animal models; this report shows the effect of AR-H067637 in venous and arterial thrombosis and bleeding models in anaesthetised rats. Thrombus formation was induced by topical application of ferric chloride to the carotid artery or to the caval vein with partial stasis. Cutaneous incision bleeding time and muscle transection blood loss were assessed, with or without acetylsalicylic acid (ASA). Activated partial thromboplastin time (APTT), ecarin coagulation time (ECT) and thrombin coagulation time (TCT) were used as plasma biomarkers of anticoagulant effect. Dalteparin was used as a reference compound. AR-H067637, given by continuous infusion, displayed a dose-dependent antithrombotic effect, with 50% inhibition (IC50) of thrombus size in venous and arterial thrombosis models obtained at plasma concentrations of 0.13 μM and 0.55 μM, respectively, without increased bleeding. Dose-dependent increased bleeding and blood loss were seen at plasma concentrations ≥1 μM AR-H067637. At the highest AR-H067637 plasma concentration tested, bleeding time and blood loss increased two and four times the vehicle group. Addition of ASA moderately potentiated bleeding time and blood loss. APTT, ECT and TCT were dose-dependently prolonged. These studies demonstrate that the DTI AR-H067637 inhibits thrombus formation in rat venous and arterial thrombosis models with no or minor increases in bleeding.

Keywords

AR-H067637 - bleeding - direct thrombin inhibitor - rat - venous and arterial thrombosis

* Footnote: Parts of this study were presented previously at the XXIst Congress of the International Society on Thrombosis and Haemostasis Geneva, 2007; Abstract P-W-637.


 

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