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Thromb Haemost 2004; 92(05): 1032-1039
DOI: 10.1160/TH04-03-0171
Platelets and Blood Cells
Schattauer GmbH

Fibrinogen adsorption and platelet adhesion to metal and carbon coatings

Lyuba I. Mikhalovska
1   School of Pharmacy & Biomolecular Sciences, University of Brighton, UK
,
Matteo Santin
1   School of Pharmacy & Biomolecular Sciences, University of Brighton, UK
,
Stephen P. Denyer
1   School of Pharmacy & Biomolecular Sciences, University of Brighton, UK
,
Andrew W. Lloyd
1   School of Pharmacy & Biomolecular Sciences, University of Brighton, UK
,
Dennis G.Teer
2   Teer Coatings Ltd., Kidderminster, UK
,
Sue Field
2   Teer Coatings Ltd., Kidderminster, UK
,
Sergey V. Mikhalovsky
1   School of Pharmacy & Biomolecular Sciences, University of Brighton, UK
› Author AffiliationsFinancial support: The authors gratefully acknowledge the support of this research by EPSRC(UK) grant GR/R315884/01
Further Information

Publication History

Received 19 March 2004

Accepted after resubmission 07 July 2004

Publication Date:
04 December 2017 (online)

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Summary

In order to study the haemocompatibility of metal and carbon coatings, fibrinogen adsorption and platelet adhesion to various coatings have been investigated.Two metallic coatings titanium and zirconium, and two carbon coatings isotropic diamondlike and isotropic graphite-like coatings, were prepared by plasma vapour deposition onto stainless steel substrate. It has been shown that the adsorption of fibrinogen to metal and carbon coatings and its post-adsorptive transition are dependent on both the material properties and the fibrinogen environment. The adsorption of fibrinogen from human plasma on titanium and zirconium coatings is similar to that on uncoated stainless steel surface. Both carbon coatings adsorb much greater amount of fibrinogen from plasma, and fibrinogen retention by carbon surfaces is also greater than by metal surfaces. Increased numbers of adhered platelets have been found on both carbon coatings in comparison to the metal materials, although this does not correlate with the amount of adsorbed fibrinogen.

Keywords

Haemocompatibility - fibrinogen - adsorption - platelets - adhesion
 

  • References

  • 1 Bailey SR. Endovascular stents: Update on stents in practice. J Long Term Eff Med Implants 2000; 10: 7-18.
    PubMedGoogle Scholar
  • 2 Mak KH, Belli G, Ellis SG. et al. Subacute stent thrombosis: evolving issues and current concepts. J Am Coll Cardiol 1996; 27: 494-503.
    CrossrefPubMedGoogle Scholar
  • 3 Keelan PC, Miyauchi K, Caplice NM. et al. Modification of molecular invents in coronary restenosis using coated stents: The Mayo Clinic ApproachReview. Seminars in Interventional Cardiology 1998; 03: 211-5.
    PubMedGoogle Scholar
  • 4 Miyamoto T, Araki T, Hiroe M. et al. Standalone cutting balloon angioplasty for the treatment of stent related restenosis. Catheterisation & Cardiovascular Intervention 2001; 54 (03) 301-8.
    PubMedGoogle Scholar
  • 5 Van Langenhove G, Kutryk MJB, Serruys PW. Pharmacological adjuncts to coronary stenting. The case of stent thrombosis. In: Handbook of Coronary Stents. Serruys PW, Kutryk MJ. eds. London: Martin Dunitz Ltd; 2000
    Google Scholar
  • 6 Rogers C, Edelman E. Endovascular stent design dictates experimental restenosis and thrombosis. Circulation 1995; 91: 2995-3015.
    CrossrefPubMedGoogle Scholar
  • 7 Virmani R, Farb A. Pathology of in-stent restenosis. Curr Opin Lipidol 1999; 10: 499-506.
    CrossrefPubMedGoogle Scholar
  • 8 Bertrand OF, Sipehia R, Mongrain R. et al. Biocompatibility aspects of new stent technology. J Am Coll Cardiol 1998; 32: 562-71.
    CrossrefPubMedGoogle Scholar
  • 9 Handbook of Coronary Stents. Serruys P, Rensing B. eds. London: Martin Dunitz Ltd; 2002
  • 10 Yun YH, Turitto VT, Daigle KP. et al. Initial hemocompatibility studies of titanium and zirconium alloys: prekallikrein activation, fibrinogen adsorption, and their correlation with the sutface electrochemical properties. J Biomed Mater Res 1996; 32: 77-85.
    CrossrefPubMedGoogle Scholar
  • 11 Gutensohn K, Beythien C, Bau J. et al. In vitro analyses of diamond-like carbon coated stents: Reduction of metal ion release, platelet activation, and thrombogenecity. Thromb Res 2000; 99: 577-85.
    CrossrefPubMedGoogle Scholar
  • 12 Jones MI, McColl IR, Grant DM. et al. Protein adsorption and platelet attachment and activation on TiN, TiC, and DLC coatings on titanium for cardiovascular application. J Biomed Mater Res 2000; 52: 423-1.
    PubMedGoogle Scholar
  • 13 Jones MI, McColl IR, Grant DM, Parker KG, Parker TL. Haemocompatibility of DLC and TiC-TiN interlayers on titanium. Diamond and Related Materials 1999; 08: 457-62.
    CrossrefPubMedGoogle Scholar
  • 14 Wang X, Zhang F, Li C. et al. Improvement of blood compatibility of artificial heart valves via titanium oxide film coated on low temperature isotropic carbon. Surface and Coatings Technology 2000; 128-129: 36-42.
    CrossrefPubMedGoogle Scholar
  • 15 Zhang F, Liu X, Mao Y. et al. Artificial heart valves: improved hemocompatibility by titanium oxide coatings prepared by ion beam assisted deposition. Surface and Coatings Technology 1998; 103-104: 146-150.
    CrossrefPubMedGoogle Scholar
  • 16 Yang P, Huang N, Leng XY. et al. In vivo study of To-O thin film fabricated by PIII. Surface and Coatings Technology 2002; 156: 284-8.
    CrossrefPubMedGoogle Scholar
  • 17 Huang N, Yang P, Leng XY. et al. Hemocompatibility of titanium oxide films. Biomaterials 2003; 24: 2177-87.
    CrossrefPubMedGoogle Scholar
  • 18 Horbett TA. Principles underlying the role of adsorbed plasma-proteins in blood interaction with foreign materials. Cardiovasc Pathol 1993; 02: S137-48.
    PubMedGoogle Scholar
  • 19 Zucker MB, Vroman L. Platelet adhesion by fibrinogen adsorbed onto glass. Proc Soc Exp Biol Med 1969; 131: 318-20.
    CrossrefPubMedGoogle Scholar
  • 20 Tsai WB, Grunkemeier JM, Horbett TA. Human plasma fibrinogen adsorption and platelet adhesion to polystyrene. J Biomed Mater Res 1999; 44: 130-9.
    CrossrefPubMedGoogle Scholar
  • 21 Nurdin N, Francois P, Mugnier Y. et al. Haemocompatibility valuation of DLCand Sic-coated surfaces. European Cells and Materials 2003; 05: 17-28.
    CrossrefPubMedGoogle Scholar
  • 22 Huang N, Yang P, Leng YX. et al. Hemocompatibility of titanium oxide films. Biomaterials 2003; 24: 2177-87.
    CrossrefPubMedGoogle Scholar
  • 23 Jones AHS, Camino D, Teer DG. et al. Novel high wear resistant diamond like carbon coatings deposited by magnetron sputtering of carbon targets. P I Mech Eng J-J Eng 1998; 212 Part J: 301-6.
    PubMedGoogle Scholar
  • 24 Yang S, Jones AHS, Camino D. et al. Deposition and tribological behavi-our of sputtered carbon hard coatings. Surf Coat Tech 2000; 124: 110-6.
    CrossrefPubMedGoogle Scholar
  • 25 Teer GD, Hampshire J, Fox V. et al. The tribological properties of MoS2/metal composite coatings deposited by closed field magnetron sputtering. Surface and Coatings Technology 1997; 94-95: 572-8.
    CrossrefPubMedGoogle Scholar
  • 26 Belitzer VA, Varetskaya TV, Butylin YP. et al. Assay of blood plasma fibrinogen. Lab Delo 1983; 04: 38-42.
    PubMedGoogle Scholar
  • 27 Mulzer SR, Brash JL. Identifications of plasma proteins adsorbed to glass from human plasma using immunoblotting methods. J Biomat Sci Polymer 1990; 01: 173-82.
    PubMedGoogle Scholar
  • 28 Tamada Y, Kulik EA, Ikada Y. Simple method for platelet counting. Biomaterials 1995; 16: 259-61.
    CrossrefPubMedGoogle Scholar
  • 29 Tsai W, Grunkemeir JM, Horbett T. Humal plasma fibrinogen adsorption and platelet adhesion to polystyrene. J Biomed Mater Res 1999; 44 (02) 130-9.
    CrossrefPubMedGoogle Scholar
  • 30 Prime K, Whitesides GM. Self-assembled organic monolayers: model systems for studying adsorption of proteins at surfaces. Science 1991; 252: 1164-7.
    CrossrefPubMedGoogle Scholar
  • 31 Elwing H, Askendal A, Lundstrom I. Competition between adsorbed fibrinogen and high-molecular-weight kininogen on solid surfaces incubated in human plasma (the Vroman effect): influence of solid surface wettability. J Biomed Mater Res 1987; 21: 1023-8.
    CrossrefPubMedGoogle Scholar
  • 32 Golander C-G, Lin Y-S, Hlady V. et al. Wetting and plasma-protein adsorption studies using surfaces with a hydrophobicity gradient. Colloids and Surfaces 1990; 49: 289-302.
    CrossrefPubMedGoogle Scholar
  • 33 Nadarajah A, Lu CF, Chittur KK. Modeling the dynamics of protein adsorption to surfaces. In: Proteins at Interfaces II. Horbett TA, Brash JL. eds. ACS, Washington: DC: 1995
    Google Scholar
  • 34 Malmsten M, Lassen B. Competitive protein adsorption at phospholipids surfaces. Colloid and Surfaces B: Biointerfaces 1995; 04: 173-84.
    CrossrefPubMedGoogle Scholar
  • 35 Horbett TA. The role of adsorbed proteins in animal cell adhesion. Colloids and Surfaces B: Biointerfaces 1994; 02: 225-40.
    CrossrefPubMedGoogle Scholar
  • 36 Rapoza RJ, Horbett TA. Postadsorptive transitions in fibrinogen: influence of polymer properties. J Biomed Mater Res 1990; 24: 1263-87.
    CrossrefPubMedGoogle Scholar
  • 37 Bohnert JL, Horbertt TA. Changes in adsorbed fibrinogen and albumin interactions with polymers indicated by decreases in detergent elutability. J Colloid Interface Sci 1986; 111: 363-77.
    CrossrefPubMedGoogle Scholar
  • 38 Slack SM, Horbett TA. Changes in fibrinogen adsorbed to segmented polyurethanes and hydroxyethylmethacrylate-ethylmethacrylate copolymers. J Biol Mater Res 1992; 26: 163349.
    PubMedGoogle Scholar
  • 39 Chinn JA, Horbett TA, Ratner BD. Baboon fibrinogen adsorption and platelet adhesion to polymeric materials. Thromb Haemost 1991; 65 (05) 608-17.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 40 Chinn JA, Posso SE, Horbett TA. et al. Postadsorptive transitions in fibrinogen adsorbed to Biomer: Changes in baboon platelet adhesion, antibody binding, and sodium dodecyl sulphate elutability. J Biomed Mater Res 1991; 25: 535-55.
    CrossrefPubMedGoogle Scholar
  • 41 Kiaei D, Hoffman AS, Horbett TA. Platelet adhesion to fibrinogen adsorbed on glow discharge-deposited polymers. In: Proteins at Interfaces II. Horbett TA, Brash JL. eds. ACS, Washington: DC: 1995
    Google Scholar
  • 42 Grunkemeier JM, Tsai WB, McFarland CD. et al. The effect of adsorbed fibrinogen, fibronectin, von Willebrand factor and vitronectin on the procoagulant state of adherent platelets. Biomaterials 2000; 21: 2243-52.
    CrossrefPubMedGoogle Scholar
  • 43 Young BR, Lambrecht LK, Cooper SL. et al. Plasma-proteins their role in initiating platelet and fibrin deposition on biomaterials. Advances in Chemistry Series 1982; 199: 317-50.
    PubMedGoogle Scholar