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Thromb Haemost 2004; 91(05): 873-878
DOI: 10.1160/TH03-05-0301
Rapid and Short Communication
Schattauer GmbH

Injection of recombinant activated factor VII can induce transient increase in circulating procoagulant microparticles

Valérie Proulle
1   INSERM Unit 143, Hôpital Bicêtre, Le Kremlin Bicêtre
2   Laboratoire d’Hématologie, AP-HP, Faculté de Médecine, Paris XI, Hôpital Bicêtre, Le Kremlin Bicêtre
,
Bénédicte Hugel
1   INSERM Unit 143, Hôpital Bicêtre, Le Kremlin Bicêtre
3   Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France
,
Benoit Guillet
2   Laboratoire d’Hématologie, AP-HP, Faculté de Médecine, Paris XI, Hôpital Bicêtre, Le Kremlin Bicêtre
,
Catherine Trichet
2   Laboratoire d’Hématologie, AP-HP, Faculté de Médecine, Paris XI, Hôpital Bicêtre, Le Kremlin Bicêtre
,
Anne Rafowicz
2   Laboratoire d’Hématologie, AP-HP, Faculté de Médecine, Paris XI, Hôpital Bicêtre, Le Kremlin Bicêtre
,
Thierry Lambert
2   Laboratoire d’Hématologie, AP-HP, Faculté de Médecine, Paris XI, Hôpital Bicêtre, Le Kremlin Bicêtre
,
Jean-Marie Freyssinet
1   INSERM Unit 143, Hôpital Bicêtre, Le Kremlin Bicêtre
3   Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France
,
Marie Dreyfus
1   INSERM Unit 143, Hôpital Bicêtre, Le Kremlin Bicêtre
2   Laboratoire d’Hématologie, AP-HP, Faculté de Médecine, Paris XI, Hôpital Bicêtre, Le Kremlin Bicêtre
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Further Information

Publication History

Received 19 May 2003

Accepted after resubmission 12 February 2004

Publication Date:
01 December 2017 (online)

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Summary

Recombinant activated factor VII (rFVIIa) is an effective haemostatic treatment in haemophiliacs with inhibitors. In vitro, FVIIa concentrations corresponding to those obtained with therapeutic doses of rFVIIa have been shown to induce normal thrombin generation and platelet activation in the absence of factors VIII or IX. To further study the in vivo haemostatic changes induced by rFVIIa, circulating procoagulant microparticles (MP) were measured in patients treated with discontinuous injections of Novoseven®. In 6 out of 15 patients, a transient peak of procoagulant MP was observed after injection, occurring 15 min to 2 h after infusion. It was composed primarily of platelet-derived MP and was of very short duration. This peak was not observed in haemophiliacs without inhibitor, who were treated with conventional replacement therapies. Our results provide further in vivo evidence that rFVIIa specifically activates platelets, either directly or as a consequence of a burst of thrombin generation that could account for its haemostatic efficacy.

Keywords

Microparticle - platelet activation - haemophilia therapy
 

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