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DOI: 10.1055/s-2008-1081334
© Georg Thieme Verlag KG Stuttgart · New York
Ascorbic Acid Induces Furanocoumarin Production in Organ Cultures of Glehnia littoralis
Publication History
Received: April 11, 2008
Revised: June 16, 2008
Accepted: June 20, 2008
Publication Date:
31 July 2008 (online)
Abstract
Exogenously supplied ascorbic acid (AsA) strongly induced furanocoumarin production in leaf and root cultures of Glehnia littoralis, but not in cell suspension cultures, after 24 h of treatment. The dose dependency showed that both organ tissues responded well to AsA supplied at concentrations of 10 – 40 mM. For induction of furanocoumarin production, roots required contact with AsA for at least 6 h and productivity markedly increased after 8 h of treatment. This is the first report of the induction of furanocoumarin biosynthesis by AsA alone and of the detection of furanocoumarin biosynthesis in a root culture system.
Abbreviations
AsA:ascorbic acid
MeJA:methyl jasmonate
IBA:Indole-3-butyric acid
MS:Murashige and Skoog
Key words
Glehnia littoralis - Apiaceae - organ culture - ascorbic acid - furanocoumarin
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- Supporting Information .
References
- 1 Larbat R, Kellner S, Specker S, Hehn A, Gontier E, Hans J. et al . Molecular cloning and functional characterization of psoralen synthase, the first committed monooxygenase of furanocoumarin biosynthesis. J Biol Chem. 2007; 282 542-54
- 2 Bourgaud F, Gravot A, Milesi S, Gontier E. Production of plant secondary metabolites: a historical perspective. Plant Sci. 2001; 161 839-51
- 3 Cole R. Light-induced cross-linking of DNA in the presence of a furanocoumarin (psoralen): studies with phage λ, Escherichia coli, and mouse leukemia cells. Biochim Biophys Acta. 1970; 217 30-9
- 4 Massot B, Milesi S, Gontier E, Bourgaud F, Guckert A. Optimized culture conditions for the production of furanocoumarins by micropropagated shoots of Ruta graveolens. Plant Cell Tiss Org Cult. 2000; 62 11-9
- 5 Ekiert H, Choloniewska M, Gomólka E. Accumulation of furanocoumarins in Ruta graveolens L. shoot cultures. Biotechnol Lett. 2001; 23 543-5
- 6 Królicka A, Staniszewska I, Bielawski K, Malinski E, Szafranek J, Lojkowska E. Establishment of hairy root cultures of Ammi majus. Plant Sci. 2001; 160 259-64
- 7 Sidwa-Gorycka M, Królicka A, Kozyra M, Glowniak K, Bourgaud F, Lojkowska E. Establishment of a co-culture of Ammi majus L. and Ruta graveolens L. for the synthesis of furanoucoumarins. Plant Sci. 2003; 165 1315-9
- 8 Ishikawa A, Kitamura , Ozeki Y, Itoh Y, Yamada A, Watanabe M. Post-stress metabolism involves umbelliferone production in anthocyanin-producing and non-producing cells of Glehnia littoralis suspension cultures. J Plant Physiol. 2005; 162 703-10
- 9 Ishikawa A, Kitamura Y, Ozeki Watanabe M. Different responses of shoot and root cultures of Glehnia littoralis to yeast extract. J Nat Med. 2007; 61 30-7
- 10 Smirnoff N. The function and metabolism of ascorbic acid in plants. Ann Bot. 1996; 78 661-9
- 11 Horemans N, Foyer C H, Potters G, Asard H. Ascorbate function and associated transport systems in plants. Plant Physiol Biochem. 2000; 38 531-40
- 12 Liso R, Tullio M CD, Ciraci S, Balestrini R, Rocca N L, Bruno L. et al . Localization of ascorbic acid, ascorbic acid oxidase, and glutathione in roots of Cucurbita maxima L. J Exp Bot. 2004; 55 2589-97
- 13 Arrigoni O, De Tullio M C. Ascorbic acid: much more than just an antioxidant. Biochim Biophys Acta. 2002; 1569 1-9
- 14 Pignocchi C, Foyer C H. Apoplastic ascorbate metabolism and its role in the regulation of cell signaling. Curr Opin Plant Biol. 2003; 6 379-89
- 15 Tamogami A, Kodama O. Coronatine elicits phytoalexins production in rice leaves (Oryza sativa L.) in the same manner as jasmonic acid. Phytochemistry. 2000; 54 689-94
Univ.-Prof. Yoshie Kitamura
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Nagasaki University
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