Horm Metab Res 2007; 39(5): 322-335
DOI: 10.1055/s-2007-977699
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

ApoA-I-binding Protein (AI-BP) and its Homologues hYjeF_N2 and hYjeF_N3 Comprise the YjeF_N Domain Protein Family in Humans with a Role in Spermiogenesis and Oogenesis

C. Rudolph 1 , A. Sigruener 1 , A. Hartmann 2 , E. Orso 1 , M. Bals-Pratsch 3 , W. Gronwald 4 , B. Seifert 3 , H. R. Kalbitzer 4 , I. Verdorfer 5 , C. M. Luetjens 6 , O. Ortmann 7 , S. R. Bornstein 8 , G. Schmitz 1
  • 1Institute of Clinical Chemistry and Laboratory Medicine, Regensburg University Medical Center, Regensburg, Germany
  • 2Institute of Pathology, Regensburg University Medical Center, Regensburg, Germany
  • 3Center for Gynecological Endocrinology, Reproductive Medicine and Human Genetics (CERMG), Regensburg, Germany
  • 4Department of Biophysics and Physical Biochemistry, University of Regensburg, Regensburg, Germany
  • 5Institute of Pathology, Medical University of Innsbruck, Innsbruck, Austria
  • 6Institute of Reproductive Medicine, University of Muenster (WHO Collaboration Centre for Research in Male Reproduction), Muenster, Germany
  • 7Department of Obstetrics and Gynecology, University of Regensburg, Caritas Hospital St. Josef, Regensburg, Germany
  • 8Department of Medicine, University of Dresden, Dresden, Germany
Further Information

Publication History

received 13. 12. 2006

accepted 16. 3. 2007

Publication Date:
29 May 2007 (online)

Abstract

The screening for additional human YjeF_N domain containing proteins beside the apolipoprotein A-I interacting protein (AI-BP), identified two other genes designated hYjeF_N2-15q23 (formerly human homologue of yeast edc3) and hYjeF_N3-19p13.11 comprising the human YjeF_N family. AI-BP is ubiquitously expressed, with a predominance of these tissues where the homologues were found to be restricted including brain, mammary gland, testes and ovaries. Immunohistochemistry of human testes and ovaries showed an expression of hYjeF_N3-19p13.11 only in Leydig cells and theca cells, respectively, indicating a role in steroid hormone metabolism. Interestingly, the protein was also strongly expressed in Leydig cell tumors and in thecofibromas. The identification of hYjeF_N2-15q23 in theca cells and granulosa cells in ovaries, in human spermatids of meiotic division part II and the apical membrane of Sertoli cells in testes suggest similar functions in oogenesis and sperm maturation which is strengthened by the identification of the spermatogenesis regulator HMGA1 as a conserved transcription factor. However, in contrast to AI-BP, both homologous proteins are unable to bind apoA-I. These results relate the human YjeF_N domain containing protein family to cholesterol processing and steroid hormone metabolism in spermiogenesis and oogenesis, and AI-BP may link this function to the HDL pathway.

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Correspondence

Prof. Dr. G. Schmitz

Institute of Clinical Chemistry and Laboratory Medicine

Regensburg University Medical Center

Franz-Josef-Strauß-Allee 11

93053 Regensburg

Germany

Phone: +49/941/944 62 01

Fax: +49/941/944 62 02

Email: gerd.schmitz@klinik.uni-regensburg.de