Planta Med 2007; 73(5): 451-460
DOI: 10.1055/s-2007-967164
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Antioxidant Activity of Vaccinium stamineum: Exhibition of Anticancer Capability in Human Lung and Leukemia Cells

Shiow Y. Wang1 , Rentian Feng2 , Linda Bowman2 , Yongju Lu2 , James R. Ballington3 , Min Ding2
  • 1Fruit Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, MD, USA
  • 2Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, USA
  • 3Horticultural Science Department, North Carolina State University, Raleigh, NC, USA
Further Information

Publication History

Received: September 5, 2006 Revised: February 12, 2007

Accepted: February 23, 2007

Publication Date:
29 March 2007 (online)

Abstract

Fruit of deerberry [Vaccinium stamineum L.] were evaluated for their antioxidant capacity and anticancer properties in JB6 P+ mouse epidermal cells, human lung and leukemia cells. Deerberries contain potent free radical scavenging activities. Pretreatment of JB6 P+ mouse epidermal cells with deerberry fruit extracts produced an inhibition on the activation of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-κB) induced by either 12-O-tetradecanoylphorbol 13-acetate (TPA) or ultraviolet-B (UVB). Deerberry fruit extracts also blocked TPA- or UVB-induced phosphorylation of ERKs and MEK œ, two upstream regulators of AP-1 and inhibited proliferation of human leukemia HL-60 cancer cells and human lung epithelial cancer A549 cells and induced apoptosis of HL-60 cells. These results suggest that the inhibition of TPA- or UVB-induced AP-1 and NF-κB activity, inhibition of HL-60 cells and cancer A549 cells proliferation and induction of apoptotic in human leukemia HL-60 cancer cells may be mediated through the ERKs and MEK œ signal pathway.

References

  • 1 Ballinger W E, Maness E P, Ballington J R. Anthocyanin and total flavonol content of Vaccinium stamineum L. Fruit.  Scientia Hortic. 1981;  15 173-8.
  • 2 Ballington J R, Kirkman W B, Ballinger W E, Maness E . Anthocyanin, aglycone, and aglycone-sugar content in the fruits of temperate North American species of four sections in Vaccinium .  J Am Soc Hortic Sci. 1988;  113 746-9.

Dr. Shiow Y. Wang

Fruit Laboratory

Beltsville Agricultural Research Center

Agricultural Research Service

U. S. Department of Agriculture

Beltsville

MD 20705-2350

USA

Fax: +1-301-504-5062

Email: wangs@ba.ars.usda.gov