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DOI: 10.1055/s-2007-967164
© Georg Thieme Verlag KG Stuttgart · New York
Antioxidant Activity of Vaccinium stamineum: Exhibition of Anticancer Capability in Human Lung and Leukemia Cells
Publication History
Received: September 5, 2006
Revised: February 12, 2007
Accepted: February 23, 2007
Publication Date:
29 March 2007 (online)
Abstract
Fruit of deerberry [Vaccinium stamineum L.] were evaluated for their antioxidant capacity and anticancer properties in JB6 P+ mouse epidermal cells, human lung and leukemia cells. Deerberries contain potent free radical scavenging activities. Pretreatment of JB6 P+ mouse epidermal cells with deerberry fruit extracts produced an inhibition on the activation of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-κB) induced by either 12-O-tetradecanoylphorbol 13-acetate (TPA) or ultraviolet-B (UVB). Deerberry fruit extracts also blocked TPA- or UVB-induced phosphorylation of ERKs and MEK œ, two upstream regulators of AP-1 and inhibited proliferation of human leukemia HL-60 cancer cells and human lung epithelial cancer A549 cells and induced apoptosis of HL-60 cells. These results suggest that the inhibition of TPA- or UVB-induced AP-1 and NF-κB activity, inhibition of HL-60 cells and cancer A549 cells proliferation and induction of apoptotic in human leukemia HL-60 cancer cells may be mediated through the ERKs and MEK œ signal pathway.
Key words
Deerberry fruit - antioxidant activity - anticancer properties - mitogen-activated protein kinase - Ericaceae - Vaccinium stamineum L.
References
- 1 Ballinger W E, Maness E P, Ballington J R. Anthocyanin and total flavonol content of Vaccinium stamineum L. Fruit. Scientia Hortic. 1981; 15 173-8.
- 2 Ballington J R, Kirkman W B, Ballinger W E, Maness E . Anthocyanin, aglycone, and aglycone-sugar content in the fruits of temperate North American species of four sections in Vaccinium . J Am Soc Hortic Sci. 1988; 113 746-9.
Dr. Shiow Y. Wang
Fruit Laboratory
Beltsville Agricultural Research Center
Agricultural Research Service
U. S. Department of Agriculture
Beltsville
MD 20705-2350
USA
Fax: +1-301-504-5062
Email: wangs@ba.ars.usda.gov