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J Reconstr Microsurg 2006; 22(6): 443-450
DOI: 10.1055/s-2006-947699
Copyright © 2006 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Survival of Long Nerve Allografts Following Donor Antigen Pretreatment: A Pilot Study

Thomas H. Tung1 , Vaishali B. Doolabh1 , Susan B. Mackinnon1 , T. Mohanakumar2 , Marshall E. Hicks3
  • 1Division of Plastic and Reconstructive Surgery, Washington University School of Medicine, St. Louis, Missouri
  • 2Department of Surgery, Washington University School of Medicine, St. Louis, Missouri
  • 3Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
Further Information

Publication History

Accepted: May 2, 2006

Publication Date:
07 August 2006 (online)

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ABSTRACT

In this study, the authors evaluated whether the pretransplant portal venous administration of UV-B irradiated donor alloantigen would induce tolerance to long peripheral nerve allografts in a swine model. They completed nerve allograft transplantation between four swine of separate lineages. Regeneration across the nerve allografts was followed for 10 months postoperatively. Sequential in vitro assays demonstrated the successful and prolonged suppression of the recipient immune response to donor antigen following antigen inoculation. Histomorphometric analysis demonstrated successful regeneration across the long nerve allografts in the pretreated recipients, but not across allografts in unimmunosuppressed recipients. A single pretransplant antigen delivery protocol has the potential to replace chronic medicinal immunosuppressant therapy and its associated morbidities. Furthermore, tolerance to long nerve allografts has immediate applicability to clinical requirements for bridging multiple, complex, long nerve gaps.

KEYWORDS

Antigen pretreatment - long nerve allograft - donor-specific immunosuppression - UV-B irradiation

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Susan E MackinnonM.D. 

Division of Plastic and Reconstructive Surgery

Suite 5401, 660 South Euclid Avenue, St. Louis, MO 63110

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