Horm Metab Res 2006; 38(4): 241-245
DOI: 10.1055/s-2006-925338
Original
© Georg Thieme Verlag KG Stuttgart · New York

Plasma Variation of Corticosteroid-binding Globulin and Sex Hormone-binding Globulin

J.  G.  Lewis1 , B.  Möpert1 , B.  I.  Shand2 , M.  P.  Doogue3 , S.  G.  Soule3 , C.  M.  Frampton3 , P.  A.  Elder1
  • 1 Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
  • 2 Lipid and Diabetes Research Group, Christchurch, New Zealand
  • 3 Department of Medicine, Christchurch Hospital, Christchurch, New Zealand
Further Information

Publication History

Received 2 August 2005

Accepted after revision 21 October 2005

Publication Date:
15 May 2006 (online)

Abstract

Sex hormone-binding globulin (SHBG) and corticosteroid-binding globulin (CBG) circulate in plasma and bind their cognate ligands with high affinity, offering a steroid delivery system to target tissues by a variety of mechanisms. Analysis of these steroid-binding proteins is gaining importance in the clinical setting, although more information is warranted on their diurnal and biological variation. This study shows that plasma SHBG (in normal subjects) exhibits little diurnal or biological variation over the 30 day period studied, in contrast to CBG, where plasma levels peak in the early afternoon. This leads to attenuation of the diurnal free cortisol level rhythm compared to total cortisol. We also show that plasma CBG is significantly lower in male subjects with the metabolic syndrome compared to age-matched lean counterparts, and may therefore act as a surrogate marker of insulin resistance. The consequence of lower levels of CBG in these obese male subjects is reflected by higher levels of circulating free cortisol, potentially offering a more favourable environment for adipogenesis.

References

  • 1 Fernández-Real J-M, Grasa M, Casamitjana R, Pugeat M, Barret C, Ricart W. Plasma total and glycosylated corticosteroid-binding globulin levels are associated with insulin secretion.  J Clin Endocrinol Metab. 1999;  84 3192-3196
  • 2 Fernández-Real J-M, Pugeat M, Grasa M, Broch M, Vendrell J, Brun J, Ricart W. Serum corticosteroid-binding globulin concentration and Insulin Resistance Syndrome: A population study.  J Clin Endocrinol Metab. 2002;  87 4680-4690
  • 3 Tchernof A, Despres J P. Sex steroid hormones, sex hormone-binding globulin, and obesity in men and women.  Horm Metab Res. 2000;  32 526-536
  • 4 Lewis J G, Shand B I, Elder P A, Scott R S. Plasma sex hormone-binding globulin rather than corticosteroid-binding globulin is a marker of insulin resistance in obese adult males.  Diabet Obes Metab. 2004;  6 259-283
  • 5 Haffner S M, Katz M S, Stern M P, Dunn J F. Relationship of sex hormone binding globulin to overall adiposity and body fat distribution in a biethnic population.  Int J Obes. 1989;  13 1-9
  • 6 Plymate S R, Matej L A, Jones R E, Friedl K E. Inhibition of sex hormone-binding globulin production in the human hepatoma (Hep G2) cell line by insulin and prolactin.  J Clin Endocrinol Metab. 1988;  67 460-464
  • 7 Geisthovel F, Olbrich M, Frorath B, Thiemann M, Weitzell R. Obesity and hypertestosteronaemia are independently and synergistically associated with elevated insulin concentrations and lipidaemia in pre-menopausal women.  Hum Reprod. 1994;  9 610-616
  • 8 Mingrone G, Greco A V, Giancaterini A, Scarfone A, Castagneto M, Pugeat M. Sex hormone-binding globulin levels and cardiovascular risk factors in morbidly obese subjects before and after weight reduction induced by diet or malabsorptive surgery.  Atherosclerosis. 2002;  161 455-462
  • 9 Bataille V, Perret B, Evans A, Amouyel P, Arveiler D, Ducimetiere P, Bard J-M, Ferrieres J. Sex hormone-binding globulin is a major determinant of the lipid profile: the PRIME study.  Atherosclerosis. 2005;  179 369-373
  • 10 Fernández-Real J-M, Pugeat M, Emptoz-Bonneton A, Ricart W. Study of the effect of changing glucose, insulin, and insulin-like growth factor-I levels on serum corticosteroid-binding globulin in lean, obese, and obese subjects with glucose intolerance.  Metabolism. 2001;  50 1248-1252
  • 11 Fernández-Real J-M, Pugeat M, López-Bermejo A, Bornet H, Ricart W. Corticosteroid-binding globulin affects the relationship between circulating adiponectin and cortisol in men and women.  Metab Clin Exper. 2005;  54 584-589
  • 12 Ousova O, Guyonnet-Duperat V, Iannucceli N, Bidanel J-P, Milan D, Genet C, Llamas B, Yerle M, Gellin J, Chardon P, Emptoz-Bonneton A, Pugeat M, Mormede P, Moisan M-P. Corticosteroid-binding globulin: A new target for cortisol driven obesity.  Mol Endocrinol. 2004;  18 1687-1696
  • 13 Wang M. The role of glucocorticoid action in the pathophysiology of the metabolic syndrome.  Nutr Metab. 2005;  2 3-16
  • 14 Hamilton-Fairley D, White D, Griffiths M, Anyaoku V, Koistinen R, Seppälä M, Franks S. Diurnal variation of sex hormone binding globulin and insulin-like growth factor binding protein-1 in women with polycystic ovary syndrome.  Clin Endocrinol. 1995;  43 159-165
  • 15 Jayagopal V, Kilpatrick E S, Jennings P E, Holding S, Hepburn D A, Atkin S L. The biological variation of sex hormone-binding globulin in Type 2 diabetes.  Diabetes Care. 2004;  27 278-280
  • 16 Jayagopal V, Kilpatrick E S, Jennings P E, Hepburn D A, Atkin S L. The biological variation of testosterone and sex hormone-binding globulin (SHBG) in polycystic ovarian syndrome: Implications for SHBG as a surrogate marker of insulin resistance.  J Clin Endocrinol Metab. 2003;  88 1528-1533
  • 17 Diver M J, Imtiaz E, Ahmad A M, Vora J P, Fraser W D. Diurnal rhythms of serum total, free and bioavailable testosterone and of SHBG in middle-aged men compared with those in young men.  Clin Endocrinol. 2003;  58 710-717
  • 18 Brien T G. Human corticosteroid-binding globulin.  Clin Endocrinol. 1981;  14 193-212
  • 19 Expert panel on detection, evaluation, and treatment of high blood cholesterol in Adults . Executive summary of the third report of the national cholesterol education program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III).  JAMA. 2001;  285 2486-2497
  • 20 Lewis J G, Longley N J, Elder P A. Monoclonal antibodies to human sex hormone-binding globulin (SHBG): Characterization and use in a simple enzyme-linked immunosorbent assay (ELISA) of SHBG in plasma.  Steroids. 1999;  64 259-265
  • 21 Lewis J G, Lewis M G, Elder P A. An enzyme-linked immunosorbent assay for corticosteroid-binding globulin using monoclonal and polyclonal antibodies: decline in CBG following synthetic ACTH.  Clin Chim Acta. 2003;  328 121-128
  • 22 Lewis J G, Manley L, Whitlow J C, Elder P A. Production of a monoclonal antibody to cortisol: application to a direct enzyme-linked immunosorbent assay of plasma.  Steroids. 1992;  57 82-85
  • 23 Coolens J-L, van Baelen H, Heyns W. Clinical use of unbound plasma cortisol as calculated from total cortisol and corticosteroid-binding globulin.  J Steroid Biochem. 1987;  26 197-202
  • 24 Barassi A, Pallotti F, Melzi d ’Eril  G V. Biological variation of procalcitonin in healthy individuals.  Clin Chem. 2004;  50 1878
  • 25 Molyneux S L, Florkowski C M, Lever M, George P M. Biological variation of coenzyme Q10.  Clin Chem. 2005;  51 455-457
  • 26 Matthews D R, Hosker J P, Rudenski A S, Naylor B A, Treacher D F, Turner R C. Homeostasis model assessment: insulin resistance and beta-cell function from fasting glucose and insulin concentrations in man.  Diabetologia. 1985;  28 412-419
  • 27 Le Roux C W, Chapman G A, Kong W M, Dhillo W S, Jones J, Alaghband-Zadeh J. Free cortisol index is better than serum total cortisol in determining hypothalamic-pituitary-adrenal status in patients undergoing surgery.  J Clin Endocrinol Metab. 2003;  88 2045-2048
  • 28 Savaridas T, Andrews P JD, Harris B. Cortisol dynamics following acute severe brain injury.  Intensive Care Med. 2004;  30 1479-1483
  • 29 Hamrahian A H, Tawakalitiu S, Oseni M D, Arafah B M. Measurements of serum free cortisol in critically ill patients.  New Engl J Med. 2004;  350 1629-1638
  • 30 Annane D, Belissant E, Cavaillon J-M. Septic shock.  Lancet. 2005;  365 63-78
  • 31 Hammond G L. Molecular properties of corticosteroid-binding globulin and the sex-steroid-binding proteins.  Endocr Rev. 1990;  11 65-79
  • 32 Rosner W. The functions of corticosteroid-binding globulin and sex hormone-binding globulin: Recent advances.  Endocr Rev. 1990;  11 80-91
  • 33 Coolens J L, Heyns W. Marked elevation and cyclic variation of corticosteroid-binding globulin: An inherited abnormality?.  J Clin Endocrinol Metab. 1989;  68 492-494
  • 34 Blum C A, Muller B, Huber P, Kraenzlin M, de Geyter C, Keller U, Puder J J. Low-grade inflammation and estimates of insulin resistance during the menstrual cycle in lean and overweight women.  J Clin Endocrinol Metab. 2005;  90 3230-3235
  • 35 Joyner J M, Hutley L J, Bachmann A W, Torpy D J, Prins J B. Greater replication and differentiation of preadipocytes in inherited corticosteroid-binding globulin deficiency.  Am J Physiol Endocrinol Metab. 2003;  284 E1049-E1054
  • 36 Wake D J, Rask E, Livingstone D EW, Söderberg S, Olsson T, Walker B R. Local and systemic impact of transcriptional up-regulation of 11β-hydroxysteroid dehydrogenase type 1 in adipose tissue in human obesity.  J Clin Endocrinol Metab. 2003;  88 3983-3988
  • 37 Lewis J G, Bagley C J, Elder P A, Bachmann A W, Torpy D J. Plasma free cortisol fraction reflects levels of functioning corticosteroid-binding globulin.  Clin Chim Acta. 2005;  359 189-194

John G. Lewis

Steroid and Immunobiochemistry Laboratory

Canterbury Health Laboratories · P.O. Box 151 · Christchurch · New Zealand

Fax: +64 (3) 36 40-889

Email: john.lewis@cdhb.govt.nz