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Horm Metab Res 2006; 38(2): 63-68
DOI: 10.1055/s-2006-925114
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Pharmacological Doses of Triiodothyronine Upregulate Lipogenic Enzyme Gene Expression in Rat White Adipose Tissue

L.  Zabrocka1 , J.  Klimek1 , J.  Swierczynski2
  • 1Department of Pharmaceutical Biochemistry, Medical University of Gdansk
  • 2Department of Biochemistry, Medical University of Gdansk
Further Information

Publication History

Received 4 July 2005

Accepted after revision 6 October 2005

Publication Date:
08 March 2006 (online)

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Abstract

Triiodothyronine (T3) is known to increase liver lipogenic enzyme gene expression both in vivo and in tissue culture. Conflicting results have been reported on the effect of T3 on lipogenic enzyme gene expression in white adipose tissue. The results presented in this paper indicate that administration of pharmacological doses of T3 in rats leads to increased fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), ATP-citrate lyase (ACL) and malic enzyme (ME) activity in white adipose tissue. The increase in lipogenic enzyme activity was associated with increased FAS, ACC, ACL and ME mRNA levels. The response was dose-dependent. Activity of lipogenic enzyme and the lipogenic enzyme mRNA levels were positively correlated to serum T3 concentration. The in vivo effect of T3 on lipogenic enzyme gene expression could be reproduced in primary white rat adipocyte culture. In conclusion, the results presented in this paper indicate that T3 exerts a stimulatory effect on lipogenic enzyme gene expression in white adipose tissue both in vivo and in tissue culture. Significant effects of T3 on lipogenic enzyme gene expression were only observed in the presence of relatively high (pharmacological) concentrations of the hormone.

Key words

Triiodothyronine - lipogenic enzyme activity - mRNA - white adipose tissue - rat adipocytes

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Julian Swierczynski

Department of Biochemistry · Medical University of Gdansk

Debinki 1 · 80-211 Gdansk · Poland

Phone: +48 (58) 349 14 62

Fax: +48 (58) 349 14 65

Email: juls@amg.gda.pl

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