Planta Med 2005; 71(12): 1152-1156
DOI: 10.1055/s-2005-873151
Original Paper
Analytical Methods
© Georg Thieme Verlag KG Stuttgart · New York

Determination of Five Coumarins in Cnidii Fructus by Micellar Electrokinetic Capillary Chromatography

Meng Hua Wu1 , Lu Hua Zhao1 , Yue Song1 , Wei Zhang2 , Bing Ren Xiang1 , Ling Hua Mei3
  • 1Key Laboratory of Modern Chinese Medicine, Ministry of Education, Nanjing, P.R. China
  • 2Guangzhou Institute for Drug Control, Guangzhou, P.R. China
  • 3Jiangxi Huiren Pharmaceutical Co., Ltd., Nanchang, P.R. China
Further Information

Publication History

Received: March 26, 2005

Accepted: May 30, 2005

Publication Date:
10 November 2005 (online)

Abstract

A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.

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Lu Hua Zhao

Key Laboratory of Modern Chinese Medicine

Ministry of Education

Nanjing

People’s Republic of China

Phone: +86-25-8327-1185

Fax: +86-25-8327-1399

Email: zhaoluhua@hotmail.com