Thorac Cardiovasc Surg 2006; 54(2): 129-133
DOI: 10.1055/s-2005-872868
Original Thoracic

© Georg Thieme Verlag KG Stuttgart · New York

Toxicity and Efficacy of Isolated Lung Perfusion with Gemcitabine in a Rat Model of Pulmonary Metastases

B. P. Van Putte1 , J. M. H. Hendriks2 , S. Romijn2 , K. De Greef2 , P. E. Y. Van Schil2
  • 1Department of Cardiothoracic Surgery, St. Antonius Hospital, Nieuwegein, The Netherlands
  • 2Department of Thoracic and Vascular Surgery, University Hospital Antwerp, Edegem, Belgium
Further Information

Publication History

Received November 4, 2005

Publication Date:
15 March 2006 (online)

Abstract

Background: Long-term toxicity and efficacy of isolated left lung perfusion (ILuP) with gemcitabine (GCB) were studied in a rat model of metastatic pulmonary adenocarcinoma. Materials: Toxicity: Forty rats were randomized into six groups and administered 160 or 320 mg/kg GCB or buffered starch, received either via intravenous injection (IV) or via ILuP. Efficacy experiment: Rats with unilateral metastases had ILuP with 320 mg/kg GCB (maximally tolerated dose administered by ILuP), while rats with bilateral metastases had an IV injection of 160 mg/kg GCB (maximally tolerated dose given by IV). Results: Toxicity experiment: After IV treatments, all rats receiving 320 mg/kg GCB died within one week, while rats who had received 160 mg/kg GCB had a survival rate of 60 %. After ILuP with 160 mg/kg GCB and 320 mg/kg GCB, survival rates were 83 % in both groups. A significant increase in collagen deposits was observed for ILuP with 320 mg/kg GCB compared to rats treated IV with 160 mg/kg GCB. Efficacy experiment: Median survival of ILuP rats treated with 320 mg/kg (38 ± 4 days) was significantly longer compared to IV rats treated with 160 mg/kg (27 ± 2 days; p = 0.02). Conclusions: ILuP with GCB prolongs survival in experimental metastatic adenocarcinoma while no major acute or long term toxicity is observed.

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MD, PhD B. P. van Putte

Department of Cardiothoracic Surgery
St. Antonius Hospital

Koekoekslaan 1

3435 CM Nieuwegein

Postbus 2500

3430 EM Nieuwegein

The Netherlands

Phone: + 31302953509

Fax: +31 3 06 09 21 20

Email: bvanputte@yahoo.com