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DOI: 10.1055/s-2004-835858
Potential Anti-Tumor Promoting Activity of Lupane-Type Triterpenoids from the Stem Bark of Glochidion zeylanicum and Phyllanthus flexuosus
Publication History
Received: February 6, 2004
Accepted: May 8, 2004
Publication Date:
10 January 2005 (online)
Abstract
Four known lupane-type triterpenoids, glochidonol (1), glochidiol (2), lup-20(29)-ene-1β,3β-diol (3) and glochidone (3) were isolated from the stem bark of Glochidion zeylanicum. Previously, lupeol (5), lup-20(29)-ene-3β,24-diol (6) and betulin (7) were isolated from the stem bark of Phyllanthus flexuosus. This study reports the assays of these lupane-type triterpenoids: all isolates 1 - 7 and synthetic analogues, glochidonyl acetate (1a), lup-20(29)-ene-1,3-dione (1b) and lup-20(29)-ene 3β,24-diacetate (6a) were tested for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol 13-acetate (TPA). Among them, the effects of compounds 2 (IC50 = 290 mol ratio/32 pmol TPA) and 3 (IC50 = 300) were stronger than the others. In addition, compound 2 exhibited a strong inhibitory effect on mouse skin tumor promotion in an in vivo mouse two-stage carcinogenesis test.
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Dr. Reiko Tanaka
Department of Medicinal Chemistry
4-20-1 Nasahara
Takatsuki
Osaka 569-1094
Japan
Phone: +81-72-690-1084
Fax: +81-72-690-1084
Email: tanakar@gly.oups.ac.jp