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Horm Metab Res 2004; 36(10): 662-666
DOI: 10.1055/s-2004-826016
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Increase of PTEN Gene Expression in Insulin Resistance

Y.  T.  Lo1 , C.  J.  Tsao2 , I.  M.  Liu3 , S.  S.  Liou3 , J.  T.  Cheng4
  • 1Department of Hospital and Health Care Administration, Chungtai Institute of Health Sciences and Technology, Taichung City, Taiwan, R.O.C.
  • 2Department of Internal Medicine, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, R.O.C.
  • 3Department of Pharmacy, Tajen Institute of Technology, Yen-Pou, Ping Tung Shien, Taiwan, R.O.C.
  • 4Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, R.O.C.
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Publication History

Received 9 March 2004

Accepted after revision 20 April 2004

Publication Date:
03 November 2004 (online)

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Abstract

Phosphatase and tensin homologue deleted from chromosome ten (PTEN) has recently been characterized as a regulator of insulin sensitivity in the insulin target tissue. However, whether PTEN gene expression is changed in insulin resistance remains unclear. We observed that both the mRNA and protein level of PTEN in soleus muscle isolated from the obese Zucker rats (fa/fa) were increased compared to the age-matched lean group. Similarly, both the mRNA and protein level of PTEN in soleus muscle of the fructose-fed lean Zucker rats (Fa/Fa) showing the higher glucose-insulin index were higher than that of the regular chow fed group. These results suggest that increase of PTEN gene expression seems to be related to the development of insulin resistance.

Key words

Glucose-insulin index · Insulin resistance · PTEN · Zucker rats

References

  • 1 Alessi D R, Downes C P. The role of PI 3-kinase in insulin action.  Biochim Biophys Acta. 1998;  1436 151-164
    PubMedSearch in Google Scholar
  • 2 Taha C, Klip A. The insulin signaling pathway.  J Membr Bio. 1999;  169 1-12
    CrossrefPubMedSearch in Google Scholar
  • 3 Moller D E, Flier J S. Insulin resistance-mechanisms, syndromes, and implications.  N Engl J Med. 1991;  325 938-948
    CrossrefPubMedSearch in Google Scholar
  • 4 Nadler S T, Stoehr J P, Rabaglia M E, Schueler K L, Birnbaum M J, Attie A D. Normal Akt/PKB with reduced PI3K activation in insulin-resistant mice.  Am J Physiol: Endocrinol Metab. 2001;  281 E1249-E1254
    PubMedSearch in Google Scholar
  • 5 Kruszynska Y T, Worrall D S, Ofrecio J, Frias J P, Macaraeg G. Olefsky JM. Fatty acid-induced insulin resistance: decreased muscle PI3K activation but unchanged Akt phosphorylation.  J Clin Endocrinol Metab. 2002;  87 226-234
    CrossrefPubMedSearch in Google Scholar
  • 6 Steck P A, Pershouse M A, Jasser S A, Yung W K, Lin H, Ligon A H, Langford L A, Baumgard ML, Hattier T, Davis T, Frye C, Hu R, Swedlund B, Teng D H, Tavtigian S V. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers.  Nat Genet. 1997;  15 356-362
    CrossrefPubMedSearch in Google Scholar
  • 7 Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang S I, Puc J, Miliaresis C, Rodgers L, McCombie R, Bigner S H, Giovanella B C, Ittmann M, Tycko B, Hibshoosh H, Wigler M H, Parsons R. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer.  Science. 1997;  275 1943-1947
    CrossrefPubMedSearch in Google Scholar
  • 8 Stambolic V, Suzuki A, de la Pompa J L, Brothers G M, Mirtsos C, Sasaki T, Ruland J, Penninger J M, Siderovski D P, Mak T W. Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN.  Cell. 1998;  95 29-39
    CrossrefPubMedSearch in Google Scholar
  • 9 Nakashima N, Sharma P M, Imamura T, Bookstein R, Olefsky J M. The tumor suppressor PTEN negatively regulates insulin signaling in 3T3-L1 adipocytes.  J Biol Chem. 2000;  275 12889-12895
    CrossrefPubMedSearch in Google Scholar
  • 10 Di Cristofano A, Kotsi P, Peng Y F, Cordon-Cardo C, Elkon K B, Pandolfi P P. Impaired Fas response and autoimmunity in Pten+/- mice.  Science. 1999;  285 2122-2125
    CrossrefPubMedSearch in Google Scholar
  • 11 Podsypanina K, Ellenson L H, Nemes A, Gu J, Tamura M, Yamada K M, Cordon-Cardo C, Catoretti G. Fisher PE, Parsons R. Mutation of Pten/Mmac1 in mice causes neoplasia in multiple organ systems.  Proc Natl Acad Sci USA. 1999;  96 1563-1568
    CrossrefPubMedSearch in Google Scholar
  • 12 Navarro-Cid J, Maeso R, Perez-Vizcaino F, Casal M C, Cachofeiro V, Ruilope L M, Tamargo J, Lahera V. Effects of antihypertensive drugs on blood pressure and metabolic alterations in the fructose-induced hypertensive rat.  Am J Hypertens. 1996;  9 669-674
    CrossrefPubMedSearch in Google Scholar
  • 13 Higashiura K, Ura N, Takada T, Li Y, Torii T, Togashi N, Takada M, Takizawa H, Shimamoto K. The effects of an angiotensin-converting enzyme inhibitor and an angiotensin II receptor antagonist on insulin resistance in fructose-fed rats.  Am J Hypertens. 2000;  13 290-297
    CrossrefPubMedSearch in Google Scholar
  • 14 Peth J A, Kinnick T R, Youngblood E B, Tritschler H J, Henriksen E J. Effects of a unique conjugate of alpha-lipoic acid and gamma-linolenic acid on insulin action in obese Zucker rats.  Am J Physiol. 2000;  278 R453-R459
    PubMedSearch in Google Scholar
  • 15 Yeh K T, Chang J G, Chen Y J, Chen S T, Yu S Y, Shih M C, Perng L I, Wang J C, Tsai M, Chang C P. Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in hepatocellular carcinoma.  Cancer Inv. 2000;  18 123-129
    PubMedSearch in Google Scholar
  • 16 Baron A D, Brechtel G. Wallace P, Edelman SV. Rates and tissue sites of non-insulin- and insulin-mediated glucose uptake in humans.  Am J Physiol. 1988;  255 E769-E774
    PubMedSearch in Google Scholar
  • 17 Hickey M S, Carey J O, Azevedo J L, Houmard J A, Pories W J, Israel R G. Dohm GL. Skeletal muscle fiber composition is related to adiposity and in vitro glucose transport rate in humans.  Am J Physiol. 1995;  268 E453-E457
    PubMedSearch in Google Scholar
  • 18 James D E, Jenkins A B, Kraegen E W. Heterogeneity of insulin action in individual muscles in vivo: euglycemic clamp studies in rats.  Am J Physiol. 1985;  248 E567-E574
    PubMedSearch in Google Scholar
  • 19 Kasiske B L, O’Donnell M P, Keane W F. The Zucker rat model of obesity, insulin resistance, hyperlipidemia, and renal injury.  Hypertension. 1992;  19 I110-1115
    PubMedSearch in Google Scholar
  • 20 Bessesen D H. The role of carbohydrates in insulin resistance.  J Nutr. 2001;  131 2782S-2786S
    PubMedSearch in Google Scholar
  • 21 Perez-Martin A, Raynaud E, Hentgen C, Bringer J, Mercier J, Brun J F. Simplified measurement of insulin sensitivity with the minimal model procedure in type 2 diabetic patients without measurement of insulinemia.  Horm Metabol Res. 2002;  34 02-106
    PubMedSearch in Google Scholar
  • 22 Butler M, McKay R A, Popoff I J, Gaarde W A, Witchell D, Murray S F, Dean N M, Bhanot S, Monia B P. Specific inhibition of PTEN expression reverses hyperglycemia in diabetic mice.  Diabetes. 2002;  51 1028-1034
    CrossrefPubMedSearch in Google Scholar
  • 23 Barzilai N, Rossetti L. Role of glucokinase and glucose-6-phosphatase in the acute and chronic regulation of hepatic glucose fluxes by insulin.  J Biol Chem. 1993;  268 25019-25025
    PubMedSearch in Google Scholar

Prof. J.-T. Cheng

Department of Pharmacology, College of Medicine, National Cheng Kung University

Tainan City · Taiwan · R.O.C.

Phone: +886 (6) 237 27 06

Fax: +886 (6) 238 65 48

Email: jtcheng@mail.ncku.edu.tw