Planta Med 2003; 69(12): 1156-1159
DOI: 10.1055/s-2003-818010
Letter
© Georg Thieme Verlag Stuttgart · New York

Diterpenes from the Aerial Parts of Salvia candelabrum and their Protective Effects against Lipid Peroxidation

Gábor Janicsák1 , Judit Hohmann2 , István Zupkó3 , Peter Forgo4 , Dóra Rédei2 , György Falkay3 , Imre Máthé1 , 2
  • 1Institute of Ecology and Botany of the Hungarian Academy of Sciences, Vácrátót, Hungary
  • 2Department of Pharmacognosy, University of Szeged, Szeged, Hungary
  • 3Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary
  • 4Department of Organic Chemistry, University of Szeged, Szeged, Hungary
This work was supported by National Scientific Fund projects No. OTKA F029249 and OTKA T035200, and by Ministry of Education grant FKFP 0024/2001
Further Information

Publication History

Received: May 28, 2003

Accepted: September 13, 2003

Publication Date:
29 January 2004 (online)

Abstract

A methanolic extract of the aerial parts of Salvia candelabrum was subjected to multiple chromatographic separation under the guidance of anti-lipid peroxidation assay. From the most active fractions seven abietane and seco-abietane diterpenes were isolated by preparative TLC purification. Besides candesalvoquinone, candelabroquinone, 12-O-methylcandesalvone B, candesalvone B methyl ester and candelabrone (all reported earlier), the known candesalvone B and the new candesalvolactone were identified. The structures were established by means of mass spectroscopy and advanced 2D NMR methods. All the identified compounds were evaluated for antioxidant activity in enzyme-dependent (IC50 values 3.49 - 10.42 μM) and enzyme-independent (IC50 values 1.40 - 13.40 μM) systems of lipid peroxidation. All compounds displayed marked concentration-dependent effects in both tests as compared with those of authentic ascorbic, rosmarinic and caffeic acids. The differences in antioxidant capacities observed in the enzyme-independent system allowed conclusions concerning structure-activity relationships.

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Dr. Gábor Janicsák

Institute of Ecology and Botany

Hungarian Academy of Sciences

2163 Vácrátót

Hungary

Fax: +36-28-360110

Email: janicsak@botanika.hu