PDF herunterladen
Planta Med 2002; 68(12): 1072-1076
DOI: 10.1055/s-2002-36356
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Yam (Dioscorea batatas) Tuber Mucilage Exhibited Antioxidant Activities in Vitro

Wen-Chi Hou1 , Feng-Lin Hsu1 , Mei-Hsien Lee1
  • 1Graduate Institute of Pharmacognosy Science, Taipei Medical University, Taipei, Taiwan
Weitere Informationen

Publikationsverlauf

Received: March 28, 2002

Accepted: August 13, 2002

Publikationsdatum:
20. Dezember 2002 (online)

    Lizenzen und Reprints

Abstract

The yam (Dioscorea batatas Decne) tuber mucilage (YTM) was extracted and partially purified by SDS and heating treatments. This purified YTM exhibited antioxidant activities in a series of in vitro tests, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (half-inhibition concentration, IC50, was 0.86 mg/mL) and hydroxyl radical (IC50 was 22 μg/mL) scavenging activity assays, reducing power test, anti-lipid peroxidation and anti-human low density lipoprotein peroxidation tests (IC50 was 145.46 μg/mL) using butylated hydroxytoluene (BHT), reduced glutathione, or ascorbic acid for comparisons. With electron paramagnetic resonance (EPR) spectrometry for DPPH radical detection, the intensities of the EPR signals were decreased by the increased amounts of YTM added (IC50 was 1.62 mg/mL). These results suggest that mucilage of yam tuber might play roles as antiradicals and antioxidants.

Key words

Antioxidant - Dioscorea batatas - Dioscoreaceae - 1,1-diphenyl-2-picrylhydrazyl (DPPH) - electron paramagnetic resonance (EPR) - hydroxyl radical - mucilage

References

  • 1 Harman D. Role of antioxidant nutrients in aging: overview.  Age. 1995;  18 51-62
    PubMedGoogle Scholar
  • 2 Ames B N. Dietary carcinogens and anticarcinogens: oxygen radicals and degenerative diseases.  Science. 1983;  221 1256-64
    CrossrefPubMedGoogle Scholar
  • 3 Gey K F. The antioxidant hypothesis of cardiovascular disease: epidemiology and mechanisms.  Biochem Soc Trans. 1990;  18 1041-5
    PubMedGoogle Scholar
  • 4 Hu C, Kitts D D. Studies on the antioxidant activity of Echinaceae root extract.  J Agric Food Chem. 2000;  48 1466-72
    CrossrefPubMedGoogle Scholar
  • 5 Rice-Evans C A, Miller N J, Paganga G. Antioxidant properties of phenolic compounds.  Trends Plant Sci.. 1997;  2 152-9
    CrossrefPubMedGoogle Scholar
  • 6 Hou W C, Chen Y C, Chen H J, Lin Y H, Yang L L, Lee M H. Antioxidant activities of trypsin inhibitor, a 33 kDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57).  J Agric Food Chem. 2001;  49 2978-81
    PubMedGoogle Scholar
  • 7 Hou W C, Lee M H, Chen H J, Liang W L, Han C H, Liu Y W, Lin Y H. Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber.  J Agric Food Chem. 2001;  49 4956-60
    CrossrefPubMedGoogle Scholar
  • 8 Misaki A, Ito T, Harada T. Constitutional studies on the mucilage of yamanoimo, Dioscorea batatas Decne, forma Tsukune. Isolation and structure of a mannan.  Agri Biol Chem. 1972;  36 761-71
    PubMedGoogle Scholar
  • 9 Tsai S S, Tai F J. Studies on the mucilage from tuber of yam (Dioscorea alata Linn.) I. Isolation and purification of the mucilage.  J Chin Agri Chem Soc. 1984;  22 88-94
    PubMedGoogle Scholar
  • 10 Lai L S, Chou S T, Chao W W. Studies on the antioxidant activities of hsian-tsao (Mesona procumbens Hemsl) leaf gum.  J Agric Food Chem. 2001;  49 963-8
    CrossrefPubMedGoogle Scholar
  • 11 Lee J M, Kwon H, Jeong H, Lee J W, Lee S Y, Baek S J, Surh Y J. Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum .  Phytother Res. 2001;  15 245-9
    CrossrefPubMedGoogle Scholar
  • 12 Halliwell B, Gutteridge J MC, Aruoma O I. The deoxyribose method: a simple test-tube assay for determination of rate constants for reactions of hydroxyl radicals.  Anal Biochem. 1987;  165 215-9
    CrossrefPubMedGoogle Scholar
  • 13 Yen G C, Chen H Y. Antioxidant activity of various tea extracts in relation to their antimutagenicity.  J Agric Food Chem. 1995;  43 27-32
    CrossrefPubMedGoogle Scholar
  • 14 Pham T Q, Cormier F, Farnworth E, Tong V H, Calsteren M V. Antioxidant properties of crocin from Gardenia jasminoides Ellis and study of reactions of crocin with linoleic acid and crocin with oxygen.  J Agric Food Chem. 2000;  48 1455-61
    CrossrefPubMedGoogle Scholar
  • 15 Yan L J, Droy-Lefaix T, Packer L. Ginkgo biloba extract (Egb 761) protects human low density lipoproteins against oxidative modification mediated by copper.  Biochem Biophys Res Commun. 1995;  212 360-6
    CrossrefPubMedGoogle Scholar
  • 16 Ohtani I I, Gotoh N, Tanaka J, Higa T, Gyamfi M A, Aniya Y. Thonningianins A and B, new antioxidants from the African medicinal herb Thonningia sanguinea .  J Nat Prod. 2000;  63 676-9
    CrossrefPubMedGoogle Scholar
  • 17 Halliwell B. Food-derived antioxidants. Evaluation of their importance in food and in vivo .  Food Sci Agric Chem. 1999;  1 67-109
    PubMedGoogle Scholar
  • 18 Esterbauer H, Schaur R G, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malondialdehyde and related aldehyde.  Free Rad Biol Med. 1991;  11 81-128
    CrossrefPubMedGoogle Scholar
  • 19 Steinbrecher U P. Oxidation of human low density lipoprotein results in derivatization of lysine residues of apolipoprotein B by lipid peroxide decomposition products.  J Biol Chem. 1987;  262 3603-8
    PubMedGoogle Scholar

Prof. Hou Wen-Chi

Graduate Institute of Pharmacognosy Science

Taipei Medical University

No. 250

Wu-Hsing Street

Taipei 110

Taiwan, ROC

eMail: wchou@tmu.edu.tw

Fax: +886-2-2378-0134

      >