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Thorac Cardiovasc Surg 2002; 50(5): 296-300
DOI: 10.1055/s-2002-34578
Original Cardiothoracic
Original Paper
© Georg Thieme Verlag Stuttgart · New York

A Novel Sialyl Lewisx Analogue Attenuates Ischemia Reperfusion Injury in Rabbit Lung

Q.  Chen1 , T.  Yoshimura1 , M.  Kawashima1 , N.  Hanaoka1 , T.  Fukuse1 , T.  Bando1 , H.  Wada1
  • 1Department of Thoracic Surgery, Faculty of Medicine, Kyoto University, Kyoto, Japan
Further Information

Publication History

Received October 18, 2001

Publication Date:
08 October 2002 (online)

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Abstract

Background: We investigated the effects of OJ-R9545, a novel Sialyl Lewisx analogue, on lung ischemia-reperfusion (IR) injury using an in vivo rabbit model. Methods: The left hilum of the lung was clamped for 110 minutes; the lung was then reperfused for 90 minutes. Either OJ-R9545 (10 mg/kg) or vehicle solution was administered from 10 minutes before reperfusion to 60 minutes after reperfusion in the OJ-R (+) and OJ-R (-) group (n = 6 in each group), respectively. The sham group (n = 3) underwent an identical procedure without ischemia. Results: Arterial oxygen tensions in the OJ-R (+) group were superior to those in the OJ-R (-) group from 30 to 90 minutes after reperfusion (p < 0.05 and p < 0.01). Lung wet/dry weight ratio and myeloperoxidase activity after reperfusion in the OJ-R (+) group were both significantly lower than the corresponding figures in the OJ-R (-) group (p < 0.05). The intrapulmonary leukocytes were significantly reduced in the OJ-R (+) group compared with those in the OJ-R (-) group (p < 0.01). Conclusions: OJ-R9545 attenuates lung IR injury by preventing leukocyte infiltration into the lung.

Key words

Sialyl Lewisx analogue - Leukocyte infiltration - Lung ischemia reperfusion injury

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Hiromi Wada MD 

Department of Thoracic Surgery, Faculty of Medicine, Kyoto University


Shogoin kawahara-cho 54

Sakyo-ku o Kyoto 606-8507

Japan

Phone: + 81-75-751-3835

Fax: + 81-75-751-4647

Email: wadah@kuhp.kyoto-u.ac.jp