J Reconstr Microsurg 2002; 18(2): 115-120
DOI: 10.1055/s-2002-19892
Copyright © 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Effect of Hyperthermic Preconditioning on the Survival of Ischemia-Reperfused Skin Flaps: A New Skin-Flap Model in the Mouse

Trinh Cao Minh1 , Shigeru Ichioka2 , Takashi Nakatsuka2 , Junsuke Kawai1 , Masahiro Shibata3 , Joji Ando3 , Kiyonori Harii1
  • 1Department of Plastic and Reconstructive Surgery, University of Tokyo, Tokyo, Japan
  • 2Department of Plastic and Reconstructive Surgery, Saitama Medical School, Saitama, Japan
  • 3Department of Biomedical Engineering, University of Tokyo, Japan
Further Information

Publication History

Publication Date:
31 January 2002 (online)

ABSTRACT

To investigate whether hyperthermic preconditioning can actually protect skin flaps against ischemia/reperfusion injury, the authors first developed a new skin-flap model in 15 mice, a dorsal bipedicle island skin-flap model. Then, another 75 mice were separated into five groups. Mice in Groups 1 to 4 received the same hyperthermic preconditioning, but had different recovery times of 6 hr, 24 hr, 48 hr, and 72 hr, respectively. Mice in Group 5 served as control. Island skin flaps were elevated in all groups, and then were subjected to 8 hr of ischemia and subsequent reperfusion. Flap survival was statistically significantly higher than in controls in animals in Groups 1 and 3, with recovery times of 6 hr and 48 hr, respectively. Mice in Groups 2 and 4 had recovery times of 24 hr and 72 hr, respectively. Hyperthermic preconditioning could thus protect skin flaps against ischemia/reperfusion injury, and there were two optimal periods for such a protective effect.

REFERENCES

  • 1 al-Qattan M M. Ischaemia-reperfusion injury: implications for the hand surgeon.  J Hand Surg . 1998;  23B 570-573
  • 2 Koenig W J, Lohner R A, Perdrizet G A. Improving acute skin-flap survival through stress conditioning using heat shock and recovery.  Plast Reconstr Surg . 1992;  90 659-664
  • 3 Lille S, Su C Y, Schoeller T. Induction of heat-shock protein 72 in rat skeletal muscle does not increase tolerance to ischemia-reperfusion injury.  Muscle Nerve . 1999;  22 390-393
  • 4 Wang B H, Ye C, Stagg C A. Improved free musculocutaneous flap survival with induction of heat shock protein.  Plast Reconstr Surg . 1998;  101 776-784
  • 5 Pohlman T H, Harlan J M. Adaptive responses of the endothelium to stress.  J Surg Res . 2000;  89 85-119
  • 6 Xi L, Chelliah J, Nayeem M A. Whole body heat shock fails to protect mouse heart against ischemia/reperfusion injury: role of 72 kDa heat shock protein and antioxidant enzymes.  J Mol Cell Cardiol . 1998;  30 2213-2227
  • 7 Dunn R M, Mancoll J. Flap models in the rat: a review and reappraisal.  Plast Reconstr Surg . 1992;  90 319-328
  • 8 Komorowska-Timek E, Newlin L, Zhang F. Shortening of rat teeth prevents autocannibalization of surgical flaps.  J Reconstr Microsurg . 1999;  15 303-306
  • 9 Yang D, Morris S F. An extended dorsal island skin flap with multiple vascular territories in the rat: a new skin flap model.  J Surg Res . 1999;  87 164-170
  • 10 Yang D, Morris S F. Comparison of two different delay procedures in a rat skin flap model.  Plast Reconstr Surg . 1998;  102 1591-1597
  • 11 Barker J H, Frank J, Bidiwala S B. An animal model to study microcirculatory changes associated with vascular delay.  Br J Plast Surg . 1999;  52 133-142
  • 12 Galla T J, Anton-Lamprecht I, Kieser M. Comparative analysis of tissue fluorescence as related to capillary perfusion in random pattern skin flaps.  Br J Plast Surg . 1992;  45 578-585
  • 13 Saetzler R K, Lehr H A, Barker J H. Visualization of nutritive perfusion following tourniquet ischemia in arterial pattern skin flaps: effect of vasoactive medication.  Plast Reconstr Surg . 1994;  94 652-660
  • 14 Plumier J C, Ross B M, Currie R W. Transgenic mice expressing the human heat shock protein 70 have improved post-ischemic myocardial recovery.  J Clin Invest . 1995;  95 1854-1860
  • 15 Palmer B. The influence of stress on the survival of experimental flaps.  Scand J Plast Reconstr Surg . 1972;  6 110
  • 16 Selye H. Ischemic necrosis: prevention by stress.  Science . 1967;  156 1262
  • 17 Qian Y Z, Shipley J B, Levasseur J E, Kukreja R C. Dissociation of heat shock proteins expression with ischemic tolerance by whole body hyperthermia in rat heart.  J Mol Cell Cardiol . 1998;  30 1163-1172
  • 18 Yamashita N, Hoshida S, Nishida M. Time course of tolerance to ischemia-reperfusion injury and induction of heat shock protein 72 by heat stress in the rat heart.  J Mol Cell Cardiol . 1997;  29 1815-1821