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Horm Metab Res 2001; 33(11): 681-686
DOI: 10.1055/s-2001-18690
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Novel Male Mice Show Gradual Decline in the Capacity to Disrupt Early Pregnancy and in Urinary Excretion of Testosterone and 17β-Estradiol During the Weeks Immediately Following Castration

E. Spironello Vella, D. deCatanzaro
  • Department of Psychology, McMaster University, Hamilton, Ontario, Canada
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Publication History

Publication Date:
04 December 2001 (online)

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Novel male mice can disrupt intrauterine implantation of fertilized ova in inseminated females. Evidence indicates mediation by androgen-dependent excretions. This study was designed to examine the time course of males' ability to disrupt pregnancy following castration and relate this to their urinary excretion of testosterone and 17β-estradiol. During days 1 - 5 of pregnancy, previously inseminated females were housed underneath castrated novel males at various intervals after the surgery. Castrated males generally continued to disrupt pregnancy during the initial weeks after surgery. Progressively, the probability of retention of pregnancy increased as a linear function of time since castration. There was an apparent asymptote, where the majority of females remained pregnant, beginning at about 6 weeks following surgery. Males’ excretion of testosterone and 17β-estradiol in urine, measured via ELISA procedures, diminished gradually during the weeks after castration.

Key words:

Bruce Effect - Castration - Mice - Implantation - Testosterone - 17β-Estradiol - Pregnancy

References

  • 1 Bruce H M. An exteroceptive block to pregnancy in the mouse.  Nature. 1959;  184 105
    PubMedGoogle Scholar
  • 2 Bruce H M. A block to pregnancy in mice caused by proximity of strange males.  J Reprod Fertil. 1960;  1 96-103
    PubMedGoogle Scholar
  • 3 deCatanzaro D, Storey A. Partial mediation of strange-male-induced pregnancy blocks by sexual activity in mice (Mus musculus).  J Comp Psychol. 1989;  103 381-388
    CrossrefPubMedGoogle Scholar
  • 4 Spironello E, deCatanzaro D. Sexual satiety diminishes the capacity of novel males to disrupt early pregnancy in inseminated female mice (Mus musculus).  J Comp Psychol. 1999;  113 218-222
    CrossrefPubMedGoogle Scholar
  • 5 Bruce H M. Further observations of the pregnancy block in mice caused by proximity of strange males.  J Reprod Fertil. 1960;  1 311-312
    PubMedGoogle Scholar
  • 6 deCatanzaro D, Muir C, Sullivan C, Boissy A. Pheromones and novel-male-induced pregnancy disruptions in mice: Exposure to females is necessary for urine alone to induce an effect.  Physiol Behav. 1999;  66 153-157
    CrossrefPubMedGoogle Scholar
  • 7 Dominic C J. The origin of the pheromone causing pregnancy block in mice.  J Reprod Fertil. 1965;  10 469-472
    PubMedGoogle Scholar
  • 8 Dominic C J. Observations on the reproductive pheromones of mice. I. Source.  J Reprod Fertil. 1966;  11 407-414
    PubMedGoogle Scholar
  • 9 Marchlewska-Koj A. Pregnancy block elicited by male urinary peptides in mice.  J Reprod Fertil. 1981;  61 221-224
    PubMedGoogle Scholar
  • 10 Parkes A S, Bruce H M. Olfactory stimuli in mammalian reproduction.  Science. 1961;  134 1049-1054
    PubMedGoogle Scholar
  • 11 Parkes A S, Bruce H M. Pregnancy-block in female mice placed in boxes soiled by males.  J Reprod Fertil. 1962;  4 303-308
    PubMedGoogle Scholar
  • 12 deCatanzaro D, Smith M, Muir C. Strange-male-induced pregnancy disruption in mice: Potentiation by administration of 17ß-estradiol to castrated males.  Physiol Behav. 1995;  58 411-414
    PubMedGoogle Scholar
  • 13 deCatanzaro D, Wyngaarden P, Griffiths J, Ham M, Hancox J, Brain D. Interactions of contact, odor cues, and androgens in strange-male-induced early pregnancy disruptions in mice (Mus musculus). .  J Comp Psychol. 1995;  109 115-122
    CrossrefPubMedGoogle Scholar
  • 14 Bruce H M. Effect of castration on the reproductive pheromones of male mice.  J Reprod Fertil. 1965;  10 141-143
    PubMedGoogle Scholar
  • 15 Rajendren G, Dominic C J. Effect of cyproterone acetate on the pregnancy-blocking ability of male mice and the possible chemical nature of the pheromone.  J Reprod Fertil. 1988;  84 387-392
    PubMedGoogle Scholar
  • 16 Manning A, Thompson M L. Postcastration retention of sexual behaviour in the male BDF1 mouse: The role of experience.  Anim Behav. 1976;  24 523-533
    PubMedGoogle Scholar
  • 17 Beach F A. Coital behavior in dogs: VI. Long-term effects of castration upon mating in the male.  J Comp Physiol Psychol (Monograph). 1970;  70 1-32
    PubMedGoogle Scholar
  • 18 Resko J A, Phoenix C H. Sexual behavior and testosterone concentrations in the plasma of the Rhesus monkey before and after castration.  Endocrinology. 1972;  91 499-502
    PubMedGoogle Scholar
  • 19 Beach F A, Holz-Tucker A M. Effects of different concentrations of androgen upon sexual behavior in castrated male rats.  J Comp Physiol Psychol. 1949;  42 433-453
    PubMedGoogle Scholar
  • 20 Davidson J M. Characteristics of sex behaviour in male rats following castration.  Anim Behav. 1966;  14 266-272
    PubMedGoogle Scholar
  • 21 Whalen R E, Beach F A, Kuehn R E. Effects of exogenous androgen on sexually responsive and unresponsive male rats.  Endocrinology. 1961;  69 373-380
    PubMedGoogle Scholar
  • 22 Bloch G J, Davidson J M. Effects of adrenalectomy and experience on postcastration sex behavior in the rat.  Physiol Behav. 1968;  3 461-465
    CrossrefPubMedGoogle Scholar
  • 23 Lisk R D, Heimann J. The effects of sexual experience and frequency of testing on retention of copulatory behavior following castration in the male hamster.  Behav Neural Biol. 1980;  28 156-171
    PubMedGoogle Scholar
  • 24 Rosenblatt J S, Aronson L R. The decline of sexual behavior in male cats after castration with special reference to the role of prior experience.  Behaviour. 1958;  12 285-338
    PubMedGoogle Scholar
  • 25 Amatayakul K, Ryan R, Uozumi T, Albert A. A reinvestigation of testicular-anterior pituitary relationships in the rat: I. Effects of castration and cryptorchidism.  Endocrinology. 1971;  88 872-880
    PubMedGoogle Scholar
  • 26 Coyotupa J, Parlow A F, Kovacic N. Serum testosterone and dihydrotestosterone levels following orchiectomy in the adult rat.  Endocrinology. 1973;  92 1579-1581
    PubMedGoogle Scholar
  • 27 Keating R J, Tcholakian R K. In vivo patterns of circulating testosterone following castration and intramuscular testosterone propionate injections of adult male rats.  Steroids. 1983;  42 63-76
    CrossrefPubMedGoogle Scholar
  • 28 Ando S, Aquila S, Beraldi E, Canonaco M, Panno M L, Valenti A, Dessi-Fulgheri F. Physiological changes in androgen plasma levels with elapsing of time from castration in adult male rats.  Horm Metab Res. 1988;  20 96-99
    PubMedGoogle Scholar
  • 29 Gupta D, Zarzycki J, Rager K. Plasma testosterone and dihydrotestosterone in male rats during sexual maturation and following orchidectomy and experimental bilateral cryptorchidism.  Steroids. 1975;  25 33-42
    CrossrefPubMedGoogle Scholar
  • 30 Gittes R F, Altwein J E, Yen S S, Lee S. Testicular transplantation in the rat: Long-term gonadotropin and testosterone radioimmunoassays.  Surgery. 1972;  72 187-192
    PubMedGoogle Scholar
  • 31 deCatanzaro D, Zacharias R, Muir C. Disruption of early pregnancy by direct and indirect exposure to novel male mice: Comparison of influences of preputialectomized and intact males.  J Reprod Fertil. 1996;  106 269-274
    PubMedGoogle Scholar
  • 32 Muir C, Vella E S, Pisani N, deCatanzaro D. Enzyme immunoassay of 17β-estradiol, estrone conjugates, and testosterone in urinary and fecal samples from male and female mice.  Horm Metab Res. 2001;  33 in press
    PubMedGoogle Scholar
  • 33 deCatanzaro D, MacNiven E, Ricciuti F. Comparison of the adverse effects of adrenal and ovarian steroids on early pregnancy in mice.  Psychoneuroendocrinology. 1991;  16 525-536
    CrossrefPubMedGoogle Scholar
  • 34 Whitney R, Burdick H. Tube locking of ova by estrogenic substances.  Endocrinology. 1936;  20 643-647
    PubMedGoogle Scholar
  • 35 deCatanzaro D, Baptista M AS, Vella E S. Administration of minute quantities of 17β-estradiol on the nasal area terminates early pregnancy in inseminated female mice.  Pharmacol Biochem Behav. 2001;  69 503-509
    CrossrefPubMedGoogle Scholar
  • 36 deCatanzaro D, Muir C, Spironello E, Binger T, Thomas J. Intense arousal of novel males in proximity to previously inseminated females: Inactivation by chlorpromazine does not diminish the capacity to disrupt pregnancy.  Psychobiology. 2000;  28 110-114
    PubMedGoogle Scholar
  • 37 Munro C J, Stabenfeldt G H, Cragun J R, Addiego L A, Overstreet J W, Lasley B L. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay.  Clin Chem. 1991;  37 838-844
    PubMedGoogle Scholar
  • 38 Pedazur E J. Multiple Regression in Behavioral Research, 2nd edition.  New York:; Holt, Rinehart and Winston, 1982
    Google Scholar
  • 39 Bardin C W, Peterson R E. Studies of androgen production by the rat: Testosterone and androstenedione content of blood.  Endocrinology. 1967;  80 38-44
    PubMedGoogle Scholar
  • 40 Hellman L, Fishman J. Oestradiol production rates in man before and after orchiectomy for cancer.  J Endocr. 1970;  46 113-114
    PubMedGoogle Scholar
  • 41 Carroll R S, Weaver C E, Baum M J. Evidence implicating aromatization of testosterone in the regulation of male ferret sexual behavior.  Physiol Behav. 1988;  42 457-460
    CrossrefPubMedGoogle Scholar
  • 42 Sinchak K, Clemens L G, Roselli C E. Levels of serum steroids, aromatase activity, and estrogen receptors in preoptic area, hypothalamus, and amygdala of B6D2F1male house mice that differ in the display of copulatory behavior after castration.  Behav Neurosci. 1996;  110 593-602
    CrossrefPubMedGoogle Scholar
  • 43 Zacharias R, deCatanzaro D, Muir C. Novel male mice disrupt pregnancy despite removal of vesicular-coagulating and preputial glands.  Physiol Behav. 2000;  68 85-290
    PubMedGoogle Scholar
  • 44 Brennan P A, Schellinck H M, Keverne E B. Patterns of expression of the immediate-early gene egr-1 in the accessory olfactory bulb of female mice exposed to pheromonal constituents of male urine.  Neuroscience. 1999;  90 1463-1470
    CrossrefPubMedGoogle Scholar
  • 45 Yamazaki K, Beauchamp G K, Bard J, Boyse E A. Sex chromosomal odor types influence the maintenance of early pregnancy in mice.  Proc Nat Acad Sci (USA). 1989;  86 9399-9401
    PubMedGoogle Scholar
  • 46 Yamazaki K, Singer A, Beauchamp G K. Origin, functions and chemistry of H-2 regulated odorants.  Genetica. 1998;  104 235-240
    CrossrefPubMedGoogle Scholar
  • 47 Pfaff D W. Estrogens and brain function.  New York:; Springer-Verlag, 1980
    Google Scholar

Denys deCatanzaro

Department of Psychology
McMaster University

Hamilton, Ontario
L8S 4K1 Canada


Email: decatanz@mcmaster.ca

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