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Horm Metab Res 2001; 33(1): 44-51
DOI: 10.1055/s-2001-12626
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Relationships of Plasma C-Peptide and Gender to the Urinary Excretion of Inositols in Older People

W. W. Campbell1 , R. E. Ostlund, Jr.3 , L. J. Joseph2 , P. A. Farrell2 , W. J. Evans1
  • 1 Department of Foods and Nutrition, Purdue University, West Lafayette, IN, USA
  • 2 Nutrition, Metabolism, and Exercise Laboratory, Donald W. Reynolds Department of Geriatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA, the Central Arkansas Veterans Healthcare System, Geriatric Research, Education, and Clinical Center, Little Rock, AR, USA
  • 3 Noll Physiological Research Center, The Pennsylvania State University, University Park, PA, USA
  • 4 Department of Medicine, Washington University, St. Louis, MO, USA
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Publication History

Publication Date:
31 December 2001 (online)

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Purpose: The urinary excretions of myo-inositol and D-chiro-inositol are elevated in diabetes, and have been suggested as possible markers or effectors of insulin action. The aim of the present study was to measure the urinary excretion of these compounds, and to assess possible relationships with the metabolic control of glucose, in older, non-diabetic men and women. Subjects: 32 older (age range 54 - 71 yrs), moderately overweight (body mass index 29.1 ± 0.4 kg/m2, mean ± SEM), non-diabetic men (n = 17) and women (n = 15). Methods: 75 g oral glucose tolerance testing was done the day after all subjects had consumed nutrient-defined menus for five days. Plasma samples were analyzed for the concentrations of glucose, insulin, and C-peptide, and the 180-minute area under the curve (AUC) for each of these compounds was calculated. Samples from 24-hour urine collections were analyzed for the concentrations of myo-inositol, D-chiro-inositol, L-chiro-inositol, and pinitol. Results: The fasting glucose, insulin, and C-peptide, and the AUC for glucose and insulin, were not different between men and women. C-peptide AUC was greater in the men versus the women (p < 0.001). The median urinary excretions (μmol/g creatinine) of myo-inositol (p < 0.001), D-chiro-inositol (p < 0.001), L-chiro-inositol (p < 0.05), and pinitol (p < 0.001) were higher, and the myo-inositol : D-chiro-inositol ratio was lower (p < 0.001), in the men versus women. For all subjects combined, C-peptide AUC was positively correlated with the urinary excretion of each of the measured inositols, as well as the myo-inositol : D-chiro-inositol ratio. The correlations between C-peptide AUC and these inositols were strongly influenced by the co-linear relationship between C-peptide AUC and gender. Conclusions: Collectively, these data show that older, moderately overweight, non-diabetic men and women with gender-related differences in glucose-stimulated C-peptide AUC, an indirect indicator of insulin secretion, also display differences in the urinary excretion of myo-inositol, D-chiro-inositol, L-chiro-inositol, and pinitol. The gender-related difference in the myo-inositol : D-chiro-inositol ratio suggests that, while the urinary excretion of all of the inositols measured were higher in the men than the women, the difference was more pronounced for D-chiro-inositol.

Key words:

Myo-Inositol - D-Chiro-Inositol - L-Chiro-Inositol - Pinitol - Insulin - C-Peptide - Obesity - Aging - Elderly

References

  • 1 Holub B J. Metabolism and function of myo-inositol and inositol phospholipids.  Ann Rev Nutr. 1986;  6 563-597
    PubMedGoogle Scholar
  • 2 Larner J, Huang L C, Schwartz C FW, et al. Rat liver insulin mediator which stimulates pyruvate dehydrogenase phosphatase contains galactosamine and D-chiroinositol.  Biochem Biophys Res Commun. 1988;  151 1416-1426
    CrossrefPubMedGoogle Scholar
  • 3 Ortmeyer H K, Bodkin N L, Hansen B C, Larner J. In vivo D-chiroinositol activates skeletal muscle glycogen synthase and inactivates glycogen phosphorylase in rhesus monkeys.  Nutritional Biochemistry. 1995;  6 499-503
    CrossrefPubMedGoogle Scholar
  • 4 Ortmeyer H K, Huang L C, Zhang L, Hansen B C, Larner J. Chiroinositol deficiency and insulin resistance. II. acute effects of D-chiroinositol administration in streptozotocin-diabetic rats, normal rats given a glucose load, and spontaneously insulin-resistant rhesus monkeys.  Endocrinology. 1993;  132 646-651
    CrossrefPubMedGoogle Scholar
  • 5 Nestler J E, Jakubowicz D J, Reamer P, Gunn R D, Allan G. Ovulatory and metabolic effects of D-chiro-inositol in the polycystic ovary syndrome.  The New England Journal of Medicine. 1999;  340 1314-1320
    CrossrefPubMedGoogle Scholar
  • 6 Ostlund R EJ, McGill J B, Herskowitz I, Kipnis D M, Santiago J V, Sherman W R. D-Chiro-inositol metabolism in diabetes mellitus.  Proceedings of the National Academy of Sciences. 1993;  90 9988-9992
    CrossrefPubMedGoogle Scholar
  • 7 Kennington A S, Hill C R, Craig J, et al. Low urinary chiro-inositol excretion in non-insulin-dependent diabetes mellitus.  New England Journal of Medicine. 1990;  323 373-378
    PubMedGoogle Scholar
  • 8 Craig J W, Larner J, Asplin C M. Chiro-inositol deficiency and insulin resistance: a clinical perspective. In: Draznin B, Le Roith D (eds). Molecular Biology of Diabetes.  Totowa, NJ:; Humana, 1994 Part II: 343-362
    Google Scholar
  • 9 Suzuki S, Kawasaki H, Satoh, et al. Urinary chiro-inositol excretion is an index marker of insulin sensitivity in Japanese type II diabetes.  Diabetes Care. 1994;  17 1465-1468
    PubMedGoogle Scholar
  • 10 Ostlund R E, Sherman W R. Measurement of D-chiro-inositol in clinical studies.  Diabetes Care. 1995;  18 1074-1075
    PubMedGoogle Scholar
  • 11 Ferrannini E, Natali A, Bell P, Cavallo-Perin P, Ningrone G. Insulin resistance and hypersecretion in obesity.  J Clin Invest. 1997;  100 1166-1173
    CrossrefPubMedGoogle Scholar
  • 12 Gavin J RI, Alberti K GMM, Davidson M B, et al. Report of the expert committee on the diagnosis and classification of diabetes mellitus.  Diabetes Care. 1997;  20 1183-1201
    PubMedGoogle Scholar
  • 13 Harris J A, Benedict F G. A biometric study of basal metabolism in man. Washington, D.C.; Carnegie Institute of Washington 1919
    Google Scholar
  • 14 Bousnes R W, Taussky H H. On the calorimetric determination of creatinine by the Jaffe reaction.  Journal of Biological Chemistry. 1945;  158 581-591
    PubMedGoogle Scholar
  • 15 Engdahl J H, Veldhuis J D, Farrell P A. Altered pulsatile insulin secretion associated with endurance training.  Journal of Applied Physiology. 1995;  79 1977-1985
    PubMedGoogle Scholar
  • 16 Wolever T MS, Jenkins D JA. The use of the glycemic index in predicting the blood glucose response to mixed meals.  American Journal of Clinical Nutrition. 1986;  43 167-172
    PubMedGoogle Scholar
  • 17 Akers R, Buskirk E R. An underwater weighing system utilizing “force cube” transducers.  Journal of Applied Physiology. 1969;  26 649-652
    PubMedGoogle Scholar
  • 18 Wilmore J H. A simplified method for determination of residual lung volumes.  J Appl Physiol. 1969;  27 96-100
    PubMedGoogle Scholar
  • 19 Siri W E. Body composition from fluid spaces and density: analysis of methods. In: Sciences NAo (ed). Techniques for measuring body composition.  Washington, D.C.:; National Academy of Sciences, 1961: 223-244
    Google Scholar
  • 20 Horwitz D L, Starr J I, Mako M E, Blackard W G, Rubenstein A H. Proinsulin, insulin, and C-peptide concentrations in human portal and peripheral blood.  Journal of Clinical Investigation. 1975;  55 1278-1283
    PubMedGoogle Scholar
  • 21 Licinio-Paizao J, Polonsky K S, Given B D, et al. Ingestion of a mixed meal does not affect the metabolic clearance rate of biosynthetic human C-peptide.  Journal of Clinical Endocrinology and Metabolism. 1986;  63 401-403
    PubMedGoogle Scholar
  • 22 Polonsky K S, Given B D, Van Cauter E. Twenty-four-hour profiles and pulsatile patterns of insulin secretion in normal and obese subjects.  Journal of Clinical Investigation. 1988;  81 442-448
    PubMedGoogle Scholar
  • 23 Asplin I, Galasko G, Larner J. Chiro-inositol deficiency and insulin resistance: a comparison of the chiro-inositol- and the myo-inositol-containing insulin mediators isolated from urine, hemodialysate, and muscle of control and type II diabetic subjects.  Proc Natl Acad Sci. 1993;  90 5924-5928
    PubMedGoogle Scholar
  • 24 Larner J, Craig J W. Urinary myo-inositol-to-chiro-inositol ratios and insulin resistance.  Diabetes Care. 1996;  19 76-78
    PubMedGoogle Scholar
  • 25 Ostlund R E, Seemayer R, Gupta S, Kimmel R, Ostlund E L, Sherman W R. A steriospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells: identification with D-chiro-[3-3H]inositol.  The Journal of Biological Chemistry. 1996;  271 10 073-10 078
    PubMedGoogle Scholar

W. W. Campbell

Department of Foods and Nutrition
1264 Stone Hall, Room 204
Purdue University

West Lafayette
IN 47907-1264
USA


Phone: Phone:+ 1 (765) 494-8236

Fax: Fax:+ 1 (765) 494-0674

Email: E-mail:campbellw@cfs.purdue.edu

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