Planta Med 2001; 67(3): 274-276
DOI: 10.1055/s-2001-12002
Letter

© Georg Thieme Verlag Stuttgart · New York

Effect of Genistein Analogs on Oxygen Radical Production in Leukocytes Stimulated by Unopsonized Zymosan

Jieun Yun1 , Sei Ryang Oh2 , Hyeong-Kyu Lee2 , IL-Moo Chang3 , Kiyoshi Takatsu4 , Yeo-Pyo Yun1 , Kyung Rak Min1 , Youngsoo Kim1,*
  • 1 College of Pharmacy, Chungbuk National University, Cheongju, Korea
  • 2 Korea Research Institute of Bioscience & Biotechnology, Taejon, Korea
  • 3 Natural Products Research Institute, Seoul National University, Seoul, Korea
  • 4 Institute of Medical Science, University of Tokyo, Tokyo, Japan
Further Information

Publication History

April 18, 2000

July 21, 2000

Publication Date:
31 December 2001 (online)

Abstract

Effects of genistein analogs on oxygen radical production have been analyzed in human neutrophils, human monocytes or murine macrophages Raw264.7 stimulated with unopsonized zymosan by lucigenin- and luminol-enhanced chemiluminescence assays. Genistein exhibited IC50 values of 10.7 - 11.5 μM on the oxygen radical production in human neutrophils, 10.9 - 11.0 μM in human monocytes, and 14.8 - 27.3 μM in Raw264.7 cells. Orobol, a genistein analog with an additional hydroxy group at the 3′ position, exhibited IC50 values of 3.0 - 3.3 μM on the oxygen radical production in human neutrophils, 2.8 - 3.1 μM in human monocytes, and 1.5 - 3.9 μM in Raw264.7 cells. Genistin and sophoricoside are genistein glycosides with a glucose moiety at 7 or 4′ position, respectively. The genistein glycosides exhibited 23 - 37 % inhibitory effects at 100 μM on the oxygen radical production.

References

  • 1 Min B, Oh S R, Lee H-K, Takatsu K, Chang I- M, Min K R, Kim Y. Sophoricoside analogs as the IL-5 inhibitors from Sophora japonica .  Planta Med.. 1999;  65 408-12
  • 2 Lim Y, Kim S H, Kim K A, Oh M W, Lee K H. Involvement of protein kinase C, phospholipase C and protein tyrosine kinase pathways in oxygen radical generation by asbestos-stimulated alveolar macrophages.  Environ. Health Perspect.. 1997;  105 1325-7
  • 3 Nagata M, Sedgwick J B, Busse W W. Synergistic activation of eosinophil superoxide anion generation by VCAM-1 and GM-CSF: Involvement of tyrosine kinase and protein kinase C.  Int. Arch. Allergy Immunol.. 1997;  114 78-80
  • 4 Wei H, Wei L, Frenkel K, Bowen R, Barnes S. Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein.  Nut. Cancer. 1993;  20 1-12
  • 5 Carreras M C, Riobo N A, Pargament G A, Boveris A, Poderoso J J. Effects of respiratory burst inhibitors on nitric oxide production by human neutrophils.  Free Radical Res.. 1997;  26 325-34
  • 6 Toda S, Shirataki Y. Inhibitory effects of isoflavones on lipid peroxidation by reactive oxygen species.  Phytother. Res.. 1999;  13 163-5
  • 7 Gallin J I, Goldstein I M, Snyderman R. Inflammation: Basic Principles and Clinical Correlates. New York; Raven Press Ltd. 1992
  • 8 Middleton E, Kandaswami C. The impact of plant flavonoids on mammalian biology: Implication for immunity, inflammation and cancer. In: Harbone JB, editor The Flavonoids. London; Chapman & Hall 1994: 619-52
  • 9 Lad P M, Kaptein J S, Lin C KE. Signal Transduction in Leukocytes: G Protein-Related and Other Pathways. Boca Raton; CRC Press 1996
  • 10 Peterson G. Evaluation of the biochemical targets of genistein in tumor cells.  J. Nut.. 1995;  125 784S-9S
  • 11 Jang D-S, Kang B-S, Ryu S Y, Chang I-M, Min K R, Kim Y. Inhibitory effects of resveratrol analogs on unopsonized zymosan-induced oxygen radical production.  Biochem. Pharmacol.. 1999;  57 705-12

Prof. Youngsoo Kim, Ph.D.

College of Pharmacy

Chungbuk National University

Cheongju 361-763

Republic of Korea

Email: youngsoo@cbucc.chungbuk.ac.kr

Fax: +82-43-268-2732