Long-term cryopreservation of nasal polyp tissue for cell culture of the primary epithelial cells

Background

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory disease of the upper respiratory tract. The epithelial cells are an important part of the pathomechanism, so that they may play an essential role for the secretion of pro-inflammatory cytokines and for the remodeling of the nasal mucosa.

Study Question

In the present work it was examined whether epithelial cells can be isolated from cryopreserved nasal polyp tissue and how the isolation and proliferation proceed in comparison to the cells which have been isolated from the fresh tissue.

Methods

The polyp tissue was packed in serum-free freezing medium containing dimethyl sulfoxide and frozen in the freezing container at -80 ° C. After 2 days it was stored in liquid nitrogen for up to 4-6 months. Epithelial cells were isolated from fresh tissue (n = 3) and the cryopreserved tissue (n = 3) by the outgrowth method and their proliferation was compared.

Results

Primary epithelial cells could be isolated from the fresh tissue as well as from the cryopreserved tissue. A comparable proliferation was found in both cell groups up to the 3rd passage.

Conclusions

In the present study, a simple and inexpensive method for cryopreservation of polyp tissue was established for a biobank. In further experiments, the production of Th2-relevant cytokines should be examined more closely.

Conflict of Interest

The author declares that there is no conflict of interest.

Publication History

Article published online:
24 May 2022