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DOI: 10.1055/s-0039-1685956
Systematic generation of primary head and neck cancer cell lines and evaluation of the treatment efficacy of drug-loaded iron oxide nanoparticles in comparison to free Chemotherapeutics
We are pursuing the aim of an individualised therapy of different head and neck cancers using the effective combination of specific chemotherapeutics-functionalized superparamagnetic iron oxide nanoparticles (SPIONs) and magnetic drug targeting (MDT), furthermore we will generate a tumor bank of primary cells that contain the full diversity of head and neck cancer. SPIONs are coated with a biocompatible substance and subsequent connected to a chemotherapeutic substance. The resulting product is applied to the vascular system which supplies the cancer and will be concentrated in the cancer by using an external magnetic field.
After collecting excess head and neck cancer tissue from patients that are treated at the ENT-Department of the University hospital in Erlangen, single cells are separated from the tissue by adding specific enzymes. Subsequently, the clones are characterised visually and by cancer cell-specific antibodies to assure a cancer cell-derived origin. Each primary cancer cell line is aliquoted and stored in liquid nitrogen. After one week, the survival of the freezing procedure, the viability of the cell line and the applicability of cell passaging is tested.
It was possible to separate different head and neck cancer cell lines. Unfortunately most of them showed a deficient growth behavior so far. It is necessary to further optimize the procedure to gain stable and sustainable cancer cell lines.
After successful establishment of the cancer cell lines, we will investigate the cellular effects after treatment with SPIONs. Furthermore, the treatment efficacy of SPIONs in combination with chemotherapeutic drugs will be examined in vitro. The project will be a great basis for future developments towards an oncologic treatment using MDT in head and neck oncology.
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Publication History
Publication Date:
23 April 2019 (online)
© 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
Georg Thieme Verlag KG
Stuttgart · New York