Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1963; 10(01): 001-008DOI: 10.1055/s-0038-1654599 Originalarbeiten — Original Article — Travaux Originaux Schattauer GmbH Microelectrophoresis of Washed and Unwashed Human Blood Platelets, with Additional Studies of Platelets from Patients with von Willebrand’s Disease and Thrombasthenia[*] Marjorie B Zucker ** 1 Division of Experimental Surgery and Physiology, Sloan-Kettering Institute for Cancer Research, New York, N. Y. , Richard U Levine 1 Division of Experimental Surgery and Physiology, Sloan-Kettering Institute for Cancer Research, New York, N. Y. › Author Affiliations Recommend Article Abstract Buy Article Summary Microelectrophoretic mobility of washed platelets suspended in isotonic saline or citrated plasma was — 0.75 and — 1.03 (µ/sec.)/(volt/cm) respectively. Erythrocytes traveled about one-third faster. There was no difference between the mobility of unwashed and washed platelets. Mobility of platelets was normal in von Willebrand5s disease. Both mobility and isoelectric point were normal in thrombasthenia. Full Text References References 1 Bangham A. D, Heard D. H, Flemans R, Seaman G. V. F. An apparatus for microelectrophoresis of small particles. Nature 182: 642 1958; 2 Seaman G. V. F, Heard D. H. A microelectrophoresis chamber of small volume for use with biological systems. Blood 18: 599 1961; 3 Ponder E, Ponder R. V. Electrophoretic mobility of red cells and their ghosts as observed with improved apparatus. J. exp. Biol 32: 175 1955; 4 Angers J, Rottino A. The electrophoretic mobility of red blood cells of normal human beings. Blood 17: 119 1961; 5 Abramson H. A. The electrophoresis of the blood platelets of the horse with reference to their origin and to thrombus formation. J. exp. Med 47: 677 1928; 6 Starlinger W, Sametnik S. Über die Entstehungsbedingungen der spontanen Venenthrombose. Klin. Wschr 06: 1269 1927; 7 Cremer H. D. Über das elektrokinetische Potential der Thrombozyten. Biochem. 2 281: 345 1935; 8 Bangham A. D, Pethica B. A, Seaman G. V. F. The charged groups at the interface of some blood cells. Biochem. J 69: 12 1958; 9 Bangham A. D. A correlation between surface charge and coagulant action of phospholipid. Nature 192: 1197 1961; 10 Bigelow F. S, Desforges J. F. Mobility of human platelets in a micro-electrophoresis cell. Amer. J. Med 14: 495 1953; 11 Ross S. W, Ebert R. V. Microelectrophoresis of blood platelets and the effects of dextran. J. clin. Invest 38: 155 1959; 12 Creger W. P, Thomas P. M. Micro-electrophoresis of human white cells and platelets. Nature 186: 171 1960; 13 Kirschmann C, Katchalsky A, de Vries A. Electrochemical study of the platelet surface. Arch. Biochem 80: 140 1959; 14 Schulte H. Beitrag zu den physikalisch-chemischen Grundlagen der Thrombose. Med. Klin 21: 1003 1926; 15 Hjort P, Rapaport I, Owren P. A. Evidence that platelet accelerator (platelet factor I) is adsorbed plasma proaccelerin. Blood 10: 1139 1955; 16 Seibert R. H, Margolius A, Ratnoff O. D. Observations on hemophilia, parahemophilia and coexistent hemophilia and parahemophilia. J. Lab. clin. Med 52: 449 1958; 17 Seligman M, Goudemand B, Janin A, Bernard J, Grabar P. Études immunochimiques sur la presence de fibrinogène dans des extraits de plaquettes humaines lavées et dans certains extraits leucocytaires. Rev. hémat 12: 302 1957; 18 Salmon J, Bounameaux Y. Étude des antigènes plaquettaires et en particulier du fibrinogène. Thrombos. Diathes. haemorrh. (Stuttg) 02: 93 1958; 19 O’Brien J. R. Some effects on blood coagulation of erythrocytes and other cells. J. Clin. Path 12: 45 1959; 20 O’Brien J. R. Platelets and the platelet-like activity of serum in blood coagulation. Proc. 7th Congr. europ. Soc. Haemat., London, 1959. part II, 667 S. Karger; Basel: 1960 21 Horowitz H. L, Spaet T. H. Generation of coagulation product I and its interaction with platelets and phospholipids. J. appl. Physiol 16: 112 1961; 22 Borchgrevink C. F. Platelet adhesion in vivo in patients with bleeding disorders. Acta med. Scand 170: 231 1961; 23 Zucker M. B. An in vitro abnormality of the blood in von Willebrand’s disease correctable by normal plasma. Nature 197: 601 1963; 24 Salzman E. W. The measurement of platelet adhesiveness. Abstracts IX. Congress International Soc. Hematol. Mexico City, Sept; 1962 25 Nilsson I. M, Blombäck M, Blombäck B. Von Willebrand’s disease in Sweden. Its pathogenesis and treatment. Acta med. Scand 164: 263 1959; 26 Cornu P, Larrieu M. J, Caen J, Bernard J. Transfusion studies in von Willebrand’s disease (Effect on bleeding time and factor VIII). Brit. J. Haemat 09: 189 1963; 27 Hardwicke J, Squire J. R. Basis of erythrocyte sedimentation rate. Clin. Sc 11: 333 1952; 28 Löhr G. W, Waller H. D, Gross R. Beziehungen zwischen Plättchenstoffwechsel und Retraktion des Blutgerinnsels. Dtsch. med. Wschr 86: 897-946 1961; 29 Krebs E. G. Glyceraldehyde-3-phosphate dehydrogenase from yeast. In: Methods in Enzymology. Ed. Colowick S. P, Kaplan N. O. Academic Press; N.Y: I 407 1955 30 Beisenherz G, Bücher T, Garbade K. H. α-glycerophosphate dehydrogenase from rabbit muscle. Ibid, p. 391. 31 Zucker M. B, Borrelli J. Changes in platelet adenosine triphosphate and phosphate distribution during viscous metamorphosis and clot retraction. Proceedings Vllth International Congress of Hematology, Tokyo. 1960. Vol. 8, part III, 623 Panpacific Press; Tokyo.: 32 Michaelis L. Der Azetat-Veronal-Puffer. Biochem. Z 234: 139 1931; 33 Borchgrevink C. F, Waaler B. A. The secondary bleeding time. A new method for the differentiation of hemorrhagic diseases. Acta med. Scand 162: 361 1958; 34 Papahadjopoulos D, Hougie C, Hanahan D. J. Influence of surface charge of phospholipids on their clot-promoting activity. Proc. Soc. exp. Biol Ill: 412 1962;
