Download PDF
Thromb Haemost 1976; 36(02): 424-429
DOI: 10.1055/s-0038-1648057
Original Article
Schattauer GmbH

Platelet Aggregation Following Defibrination with Ancrod

C Lawrence Slade
1   United States Army Institute of Surgical Research, Brooke Army Medical Center, Fort Sam Houston, Texas 78234, U.S.A.
,
W Abe Andes
1   United States Army Institute of Surgical Research, Brooke Army Medical Center, Fort Sam Houston, Texas 78234, U.S.A.
,
Arthur D. Mason Jr.
1   United States Army Institute of Surgical Research, Brooke Army Medical Center, Fort Sam Houston, Texas 78234, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 06 February 1976

Accepted 07 May 1976

Publication Date:
03 July 2018 (online)

    Permissions and Reprints

Summary

In vitro platelet aggregation was studied in dogs following defibrination with Ancrod. There was marked inhibition of aggregation immediately after defibrination. A gradual return toward normal aggregation was observed over the 96 hours study period, but aggregation was still abnormal at 96 hours after defibrination. There was an inverse and highly significant correlation between aggregation and fibrin degradation product level. Fibrinogen was not measurable after defibrination for the entire study period. Aggregation was thus independent of fibrinogen concentration in this study.

 

  • References

  • 1 Ashford A, Ross J. W, Southgate P. 1968; Pharmacology and toxicology of a defibrinating substance from malayan pit viper venom. Lancet 486.
    PubMedGoogle Scholar
  • 2 Bell W. R, Pitney W. R, Goodwin J. F. 1968; Therapeutic defibrination in the treatment of thrombotic disease. Lancet 490.
    PubMedGoogle Scholar
  • 3 Bell W. R, Bolton G, Pitney W. R. 1968; The effect of arvin on blood coagulation factors. British Journal of Haematology 15: 589.
    CrossrefPubMedGoogle Scholar
  • 4 Brecher G, Cronkite E. P. 1950; Morphology and Enumeration of Human Blood Platelets. Journal of Applied Physiology 3: 365.
    CrossrefPubMedGoogle Scholar
  • 5 Brown C. H, Bell W. R, Shreiner D. P, Jackson D. P. 1972; Effects of arvin on blood platelets. In vitro and in vivo studies. Journal of Laboratory and Clinical Medicine 79: 758.
    PubMedGoogle Scholar
  • 6 Browse N. L. 1974. Vein surgery during defibrination. In: Martin M, Schoop W. (ed), Aktuelle Probleme in der Angiologie: 26, Defibrinierung mit thrombinähnlichen Schlangengiftenzymen. Hans Huber, Bern; Stuttgart, Wien.:
    Google Scholar
  • 7 Clauss A. 1957; Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta Hematologia 17: 237.
    PubMedGoogle Scholar
  • 8 Davey M. G, Lüscher E. F. 1967; Actions of thrombin and other coagulent and protelytic enzymes on blood platelets. Nature 216: 857.
    CrossrefPubMedGoogle Scholar
  • 9 Esnouf M. P, Tunnah G. W. 1967; The isolation and properties of the thrombin-like activity from ancistrodon rhodostoma venom. British Journal of Haematology 13: 581.
    CrossrefPubMedGoogle Scholar
  • 10 Garvey M. B, Black J. M. 1972; The detection of fibrinogen/fibrin degradation products by means of a new antibody coated latex particle. Journal of Clinical Pathology 25: 680.
    CrossrefPubMedGoogle Scholar
  • 11 Harker L. A. 1974. Hemostasis Manual. F. A. Davis Co.; Philadelphia: 68.
    Google Scholar
  • 12 Prentice C. R. M, Hassanein A. A, Turpe A. G. G, McNicol G. P, Douglas A. S. 1969; Changes in platelet behavior during Arvin Therapy. Lancet 644.
    PubMedGoogle Scholar
  • 13 Reid H. A, Chan K. E. 1968; The paradox in therapeutic defibrination. Lancet 485.
    PubMedGoogle Scholar
  • 14 Sharp A. A, Warren B. A, Paxton A. M, Allington M. J. 1968; Anticoagulation therapy with a purified fraction of malayan pit viper venom. Lancet 493.
    PubMedGoogle Scholar
  • 15 Smith R. T, Tsao C. 1973; Fibrin degradation products in the postoperative period. American Journal of Clinical Pathology 60: 644.
    CrossrefPubMedGoogle Scholar