CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S182
DOI: 10.1055/s-0038-1640343
Poster
Otologie: Otology

Cytokeratin expression pattern in acquired cholesteatoma versus meatal skin; an immunochemical study

M Hamed
1   Sohag University, Sohag, Egypt
,
R Sayed
2   ORL Dept., Faculty of Medicine, Sohag, Egypt
,
K Shiogama
3   Pathology Dept., Fujita Health University, Toyoake, Japan
,
S Nakata
4   ORL Dept., Fujita Health University School of Medicine, Nagoya, Japan
,
B Badawy
2   ORL Dept., Faculty of Medicine, Sohag, Egypt
,
Y Tsutsumi
3   Pathology Dept., Fujita Health University, Toyoake, Japan
› Author Affiliations
› Further Information
Also available at
 
 

    Introduction:

    Cholesteatoma is a proliferative disease of the middle ear with bone resorption activity. Its pathogenesis remains controversial and has yet to be clarified. Cytokeratins represent a well-known epithelial proliferative and differentiation markers. In the current study, we investigated expression of a large set of cytokeratins in cholesteatoma matrix versus deep meatal skin tissues from the same patients.

    Methodology:

    An immunohistochemical study was carried out using acquired cholesteatoma (n = 15) and deep meatal skin tissues (n = 6). Mouse monoclonal antibodies were used against cytokeratin 5/6 (CK5/6), cytokeratin HMW (CKHMW), Cytokeratin 10 (CK10), Cytokeratin 14 (CK14) and Cytokeratin19 (CK19). The indirect immunoperoxidase method for immunostaining was employed. Both pattern and intensity of expression of those cytokeratins were evaluated and compared in cholesteatoma and skin tissues.

    Results:

    With the exception of CK19, all studied cytokeratins were expressed in cholesteatoma matrix and epidermis of meatal skin. In addition, a typical pattern of expression was observed between both tissues. No significant difference in the intensity of expression was found (p > 0.05). CK HMW and CK5/6 were expressed in all layers of cholesteatoma epithelium and meatal skin. CK10 was expressed in suprabasal keratinocytes whereas CK14 was expressed in basal, parabasal and to lesser extent in suprabasal cholesteatoma and skin layers.

    Conclusions:

    Cytokeratins are similar in their distribution in both skin and cholesteatoma tissues. These findings support the immigration theory and confirm the proliferative nature of cholesteatoma. However, more research is needed to clarify how this knowledge can affect the future management of acquired cholesteatoma.


    #

    No conflict of interest has been declared by the author(s).

    Dr. Mahmood Hamed
    Sohag University,
    Faculty of Medicine, Sohag University, 82524,
    Sohag,
    Egypt   

    Publication History

    Publication Date:
    18 April 2018 (online)

    © 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

    Georg Thieme Verlag KG
    Stuttgart · New York