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Vet Comp Orthop Traumatol 2003; 16(04): 255-259
DOI: 10.1055/s-0038-1632781
Original Research
Schattauer GmbH

Canine keratinocyte culture and use of a cultured epidermal autograft in a dog

D. M. Anderson
1   Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom
,
M. A. Stanley
2   Epithelial Pathology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge
,
R. A. S. White
1   Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom
› Author AffiliationsD. M. Anderson was in receipt of a Wellcome Trust Veterinary Clinical Research Training Scholarship.
Further Information

Publication History

Received 20 October 2002

Accepted 17 February 2003

Publication Date:
22 February 2018 (online)

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Summary

A seven month old female Yorkshire Terrier was admitted with severe contracture and scarring of the ventral abdomen, thorax and limbs, after a scald injury. Surgical management consisted of multiple releasing and reconstruction skin flaps. Keratinocytes, harvested from a 1 cm2 full thickness skin biopsy, were cultured on a lawn of irradiated feeder cells, and used as a cultured epithelial autograft on the remaining skin deficits. Epithelialisation of the deficits was complete at the first dressing change, five days after application. This is the first report of successful culture of canine keratinocytes suitable for use in skin reconstruction in a clinical case.

Keywords

Keratinocyte - graft - burn
 

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