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Thromb Haemost 2001; 85(02): 226-230
DOI: 10.1055/s-0037-1615702
Review Article
Schattauer GmbH

Lack of Association between the Platelet Glycoprotein Ia C807T Gene Polymorphism and Myocardial Infarction in Japanese

An Approach Entailing Melting Curve Analysis with Specific Fluorescent Hybridization Probes
Hiroyuki Morita
1   Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
,
Hiroki Kurihara
1   Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
,
Yasushi Imai
1   Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
,
Takao Sugiyama
2   The Institute for Adult Diseases Asahi Life Foundation, University of Tokyo, Tokyo, Japan
,
Chikuma Hamada
3   Department of Pharmacoepidemiology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
,
Eiichi Sakai
4   Nihon Gene Research Laboratories Inc, Sendai, Japan
,
Mitsuko Mori
4   Nihon Gene Research Laboratories Inc, Sendai, Japan
,
Ryozo Nagai
1   Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

Received 18 February 2000

Accepted after resubmission 30 August 2000

Publication Date:
08 December 2017 (online)

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Summary

The platelet-collagen receptor, glycoprotein Ia/IIa (integrin α2β1) plays a fundamental role in the adhesion of platelets to fibrillar collagen, an event leading to platelet activation and thrombus formation and contributing to the pathogenesis of thrombotic disease. Further, glyco-protein Ia/IIa receptor density and function may be associated with two linked and silent polymorphisms (807C/T and 873G/A) within the glyco-protein Ia gene. We tested the extent to which these polymorphisms serve as genetic markers of myocardial infarction in a Japanese population. A case-control study was carried out using 210 Japanese myocar-dial infarction patients and 420 age- and sex-matched controls. Geno-typing was accomplished using PCR followed by melting curve analysis with specific fluorescent hybridization probes. The 807CC, CT, TT genotypes linked perfectly to the 873GG, GA, AA genotypes, respectively. Allele frequencies of the 807T (873A) variant were similar in the control and patient groups (0.373 vs. 0.352). The 807T and 873A variants of platelet glycoprotein Ia gene are common and in a perfect linkage in the Japanese population, but it appears unlikely that the 807T (873A) variant represents a useful marker of increased risk for myocardial infarction.

Keywords

Platelet glycoprotein - myocardial infarction - melting curve analysis - genetics - ethnic difference
 

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