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Thromb Haemost 1999; 82(05): 1497-1503
DOI: 10.1055/s-0037-1614861
Rapid Communications
Schattauer GmbH

Natriuretic Peptides Regulate the Expression of Tissue Factor and PAI-1 in Endothelial Cells

Masami Yoshizumi
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Hajime Tsuji
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Hiromi Nishimura
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Haruchika Masuda
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Yasushi Kunieda
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Hidehiko Kawano
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Shinzo Kimura
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Tatsuya Sugano
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Hidetsugu Kitamura
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Katsumi Nakagawa
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Masao Nakagawa
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
› Author Affiliations
Further Information

Publication History

Received 03 November 1998

Accepted after resubmission 29 June 1999

Publication Date:
09 December 2017 (online)

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Summary

In the present study, we demonstrate that brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) interact with angiotensin II (Ang II) in regulative blood coagulation and fibrinolysis by suppressing the expressions of both tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) induced by Ang II. The expressions of TF and PAI-1 mRNA were analyzed by northern blotting methods, and the activities of TF on the surface of rat aortic endothelial cells (RAECs) and PAI-1 in the culture media were respectively measured by chromogenic assay.

Both BNP and CNP suppressed the expressions of TF and PAI-1 mRNA induced by Ang II in a time- and concentration-dependent manner via cGMP cascade, which suppressions were accompanied by respective decrease in activities of TF and PAI-1. However, neither the expression of tissue factor pathway inhibitor (TFPI) nor tissue-type plasminogen activator (TPA) mRNA was affected by the treatment of BNP and CNP.

Key words

BNP - CNP - Ang II - coagulation - fibrinolysis
 

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