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Thromb Haemost 2000; 84(03): 429-435
DOI: 10.1055/s-0037-1614040
Commentary
Schattauer GmbH

Macrophages Synthesize Factor X and Secrete Factor X/Xa-containing Prothrombinase Activity into the Surrounding Medium

Gunnar Pejler
1   From the Swedish University of Agricultural Sciences, Department of Veterinary Medical Chemistry, The Biomedical Center, Uppsala, Sweden
,
Carolina Lunderius
2   Uppsala University, Department of Cell and Molecular Biology, Uppsala, Sweden
,
Bianca Tomasini-Johansson
1   From the Swedish University of Agricultural Sciences, Department of Veterinary Medical Chemistry, The Biomedical Center, Uppsala, Sweden
3   University of Wisconsin-Madison, Department of Medicine, Madison, USA
› Author AffiliationsThis work was supported by grants from the Swedish Medical Research Council (grant no. 9913) and from King Gustaf V’s 80th anniversary Fund.
We are grateful to Peter McCourt for linguistic revision of the manuscript.
Further Information

Publication History

Received 24 December 1999

Accepted after revision 27 March 2000

Publication Date:
14 December 2017 (online)

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Summary

Activation of the coagulation cascade, mediated by various monocyte/macrophage procoagulants, is an important component in the pathology of inflammatory disease. The type of procoagulant expressed may vary between different monocyte/macrophage subtypes and may differ depending on how the cells are treated. In the present study we show that both murine peritoneal macrophages and human adherent synovial cells from rheumatoid arthritis lesions express prothrombinase activity that was inhibited by anti-Factor X antibodies. Northern blot analysis showed that Factor X was transcribed by the murine peritoneal cells and Western blot analysis showed the presence of Factor X antigen. Further experiments showed that the prothrombinase activity was secreted by the cells into the medium in a detergent-sensitive form, suggesting that the prothrombinase is released on small lipid-containing vesicles.

Key words

Macrophage - prothrombinase - Factor X - thrombin
 

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