Development of a novel quality control method for estimation of berberine in Tinospora cordifolia by HPTLC fingerprint approach

A straightforward, delicate and fast elite thin layer chromatographic system (High performance thin layer chromatography; HPTLC) has been produced and validated [1 – 4] for quantitative determination of berberine in Tinospora cordifolia (Thunb.) Miers. [Menispermaceae]. The chromatographic development was completed on HPTLC plates precoated with silica gel 60 F 254 utilizing a blend of methanol, acetic acid and water (8:1:1) (v/v/v) as mobile phase. Detection was completed densitometrically at 366nm. The Rf estimation of berberine was observed to be 0.71 ± 0.02. The system was validated according to ICH (The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use) [5] guidelines regarding linearity, precision, accuracy, robustness and so forth. The calibration curve was observed to be straight over a scope of 120 – 360 ng/spot with a regression coefficient of 0.971. The accuracy was observed to be high as 98.56% and % RSD values for intra-day and between day variations were under 2%. The system showed high affectability and specificity. The strategy is new, basic and monetary for routine estimation of berberine in T. cordifolia plant tests and pharmaceutical definitions to help the commercial ventures and in addition scientists for their touchy determination of critical phytoconstituent berberine quickly requiring little to no effort in routine examination.

Acknowledgements: Financial support from University Grants Commission (Ministry of Human Resources and Development, Government of India) is acknowledged.

Keywords: HPTLC; validation; Tinospora cordifolia; berberine.

References:

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No conflict of interest has been declared by the author(s).