Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1597041
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Isolation and profiling of coumarin glycosides from the anti-hyperuricemic ethanol extract of mulberry (Morus alba L.) twigs (Chin.Ph.)

K Kuchta
1   National Institute of Health Sciences, Division of Pharmacognosy, Phytochemistry and Narcotics, Tokyo, Japan
,
HH He
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
JB Yao
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
3   Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou, China
,
W Ai
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
JF Wang
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
J Wu
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
JN Hu
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
RW Wang
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
3   Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou, China
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 
 

    In Traditional Chinese Medicine (TCM) dried twig of Morus alba L. are extensively used as an antiasthmatic and antirheumatic agent. In order to identify constituents contributing to this activity, the raw drug was extracted twice with 60% ethanol under reflux for 2h. The resulting extract was evaporated to dryness under reduced pressure and subjected to column chromatography (ODS, gradient H2O/MeOH (20:1, 10:1, 5:1, 1:1, 0:1, v/v)). The fraction eluted with 20% MeOH was subjected to HPLC/UV analysis, and then was analyzed by LC/ESI-MS (Xtimate XB-C18 column (5 µm, 4.6 × 250 mm); acetonitrile, water, methanol gradient; 1 ml/min; 25 °C; DAD 324nm). On the basis of the fragmentation behaviour of known coumarin glycosides isolated, one new compound, 6-hydroxyl-7-O-glucopyrano-xylopyranosyl coumarin (1), along with one known compound, 6,7-di-O-glucopyra-nosylaesculetin, were detected and identified. In addition to the mass spectral data, we also report NMR data for the two compounds which allowed the completion of their structural elucidation. Furthermore, the known constituents of Mori ramulus (Chin.Ph.) umbelliferone-7-β-D-apiofuranosyl-(1-> 6)-β-D-glucopyranoside, 7-[[6-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl]oxy]-2 H-1-benzopyran-2-one, Xeroboside, Scopolin, and 7-[(6-O-D-apio-β-D-furanosyl-β-D-glucopyranosyl)oxy]-5-hydroxy-2 H-1-benzopyran-2-one were rediscovered.

    Zoom Image
    Fig. 1: 6-hydroxyl-7-O-glucopyrano-xylopyranosyl coumarin (1)

    Keywords: Morus alba L., anti-hyperuricemic activity, coumarin glycosides.


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    No conflict of interest has been declared by the author(s).

     
    Zoom Image
    Fig. 1: 6-hydroxyl-7-O-glucopyrano-xylopyranosyl coumarin (1)