Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596897
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Antiproliferative potential of dried wild pear tea before and after in vitro simulated digestion

KP Šavikina
1   Institute for Medicinal Plants Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
,
JČ Živković
1   Institute for Medicinal Plants Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
,
NS Stanisavljević
2   Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11000 Belgrade, Serbia
,
NĐ Grozdanić-Stanisavljević
3   Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia
,
TP Stanojković
3   Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia
,
GM Zdunić
1   Institute for Medicinal Plants Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
,
JT Samardžić
2   Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11000 Belgrade, Serbia
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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    Due to high consumption of pears (Pyrus spp.) among European nations they can be considered as important source of antiproliferative phenolics compounds. One of the limiting factors for phenolics beneficial effects is their bioavailability which depends on their digestive stability and release from food matrix [1]. In the present study decoction obtained from dried wild pear fruits was subjected to in vitro simulated digestion in the presence of food matrix in order to determine changes in polyphenol content and antiproliferative activity. The content of total phenolic compounds (TPC) and total proanthocyanidins (TPA) were determined spectrophotometrically, while quantification of individual phenolics in investigated sample and its digested fractions was carried out using HPLC method. Addition of food matrix rapidly decreased TPC by 69.26%. After in vitro digestion it was slightly elevated but still lower compared to non-digested decoction. This indicates that food matrix immediately binds high percent of soluble phenolics from investigated sample. Chlorogenic acid was the major compound identified in wild pear tea, while hyperoside, quercitrin and isoquercitrine were determined to a lesser extent. After in vitro digestion chlorogenic acid content decreased from 42.23 to 6.07 µg/mL while quercetin derivatives were detected only in traces.

    Antiproliferative activity was investigated on human epithelial carcinoma cell line (HeLa) and human fetal lung fibroblast (MRC-5) cells. The inhibition of proliferation of HeLa cells in the presence of digested decoction increased approximately 1.5-fold in comparison to non-digested sample, with IC50 value decreasing from 37.94 to 26.19 µg/mL. On the other hand, investigated samples did not show any cytotoxic effect on normal MRC-5 cell line at concentration lower than 200 µg/mL.

    Acknowledgements: To Ministry of Education, Science and Technology of Serbia, project number 46013.

    Keywords: Pyrus, wild pear, tea, phenolics, digestion, antiproliferative

    References:

    [1] Palafox-Carlos H, Ayala-Zavala JF, González-Aguilar GA. The role of dietary fiber in the bioaccessibility and bioavailability of fruit and vegetable antioxidants. J Food Sci 2011; 76: 6 – 15


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    No conflict of interest has been declared by the author(s).