Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596870
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Proteomic analysis of the anti-obesity effect of Taeumjowui-Tang in high fat diet-induced obese mice

E Changkyun Park
1   Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), 34133 Daejeon, Republic of Korea
2   Department of Bio-Analytical Science, University of Science and Technology (UST), 34113 Daejeon, Republic of Korea
,
SY Lee
1   Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), 34133 Daejeon, Republic of Korea
,
SH Yun
1   Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), 34133 Daejeon, Republic of Korea
,
WY Kim
1   Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), 34133 Daejeon, Republic of Korea
,
Y Yi
1   Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), 34133 Daejeon, Republic of Korea
,
GH Kim
1   Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), 34133 Daejeon, Republic of Korea
3   Department of Functional Genomics, University of Science and Technology (UST), 34113 Daejeon, Republic of Korea
,
SI Kim
1   Division of Bioconvergence Analysis, Korea Basic Science Institute (KBSI), 34133 Daejeon, Republic of Korea
2   Department of Bio-Analytical Science, University of Science and Technology (UST), 34113 Daejeon, Republic of Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 
 

    Taeumjowi-tang (Taiyintiaowei-tang) is a traditional Korean medicinal extract consisting of eight herbs (Coicis semen, Castaneae semen, Raphani semen, Schizandrae fructus, Liriopis tuber, Acori graminei rhizome, Platycodi radix, and Ephedrae herba) and widely used verbal remedy for obesity [1,2]. However, its downstream mechanism of anti-obesity effect is poorly understood. Here we investigated the anti-obesity role of Taeumjowi-tang by extensive proteomic and bioinformatic analysis. Mice were fed with high fat diet (HFD) with or without extract of Taeumjowi-tang. Green tea extract was used for positive control. Experimental groups were (1) AIN-76A normal diet (ND), (2) HFD, (3) HFD + green tea (GT), and (4) HFD + Taeumjowi-tang (TE). After 16 weeks of diet, mice fed with TE showed reduced body weight. Liver and fat tissue weight also decreased in TE group. Furthermore, mice of TE group showed lower fasting glucose level and improved glucose and insulin sensitivity. In order to investigate the underlying mechanism of anti-obesity effect of TE, the livers were isolated and hepatic proteome was examined by LC-MS/MS. We found 1,119, 1,143, 1,120 and 1,056 proteins in ND, HFD, HFD+GT, and HFD+TE, respectively. From the result, we noticed that protein expression in HFD+TE is considerably different from that in HFD+GT. This suggests that mechanism of anti-obesity effect of TE is different from that of GT. Extensive bioinformatics analysis would provide us valuable information for possible function and mechanisms of TE.

    Keywords: Taeumjowi-tang, obesity, proteomics.

    References:

    [1] Park S, Park JS, Cheon C, Yang YJ, An C, Jang HB, Song YK, Go H, Lee JA, Shin Y, Ko SG. A pilot study to evaluate the effect of Taeumjowi-tang on obesity in Korean adults: study protocol for a randomised, double-blind, placebo-controlled, multicentre trial. Trials 2012; 13: 33

    [2] Yoo JH, Lee EJ, Kwak CK, Sohn EH, Koh BK, Song IB, Lee KS. Clinical trial of herbal formula on weight loss in obese Korean children. Am J Chin Med 2005; 33: 713 – 722


    #

    No conflict of interest has been declared by the author(s).