Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596798
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Glycyrrhizin production in hairy root cultures of Glycyrrhiza uralensis induced triterpenoid biosynthetic gene

M Kojoma
1   Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu, Hokkaido 061 – 0293, Japan
,
H Seki
2   Department of Biotechnology, Osaka University, 2 – 1 Yamadaoka, Suita-shi, Osaka 565 – 0871, Japan
4   Center for Sustainable Resource, RIKEN, 1 – 7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230 – 0045, Japan
,
K Ohyama
3   Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2 – 12 – 1 Ookayama, Meguro-ku, Tokyo 152 – 8550, Japan
4   Center for Sustainable Resource, RIKEN, 1 – 7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230 – 0045, Japan
,
SY Kim
1   Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu, Hokkaido 061 – 0293, Japan
,
T Muranaka
2   Department of Biotechnology, Osaka University, 2 – 1 Yamadaoka, Suita-shi, Osaka 565 – 0871, Japan
4   Center for Sustainable Resource, RIKEN, 1 – 7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230 – 0045, Japan
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Publikationsdatum:
14. Dezember 2016 (online)

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    Glycyrrhiza uralensis Fisch. (Licorice) is widely used as a natural herbal drug in traditional oriental medicine. Some compounds found in G. uralensis are also used as sweeteners and flavorings. Glycyrrhizin, a triterpenoid saponin derived from the underground parts of G. uralensis, is a major bioactive compound that has several pharmacological activities. As a resource for gene discovery in glycyrrhizin biosynthesis, an expressed sequence tags (ESTs) library was generated, which is composed of 56,857 cDNAs from the rhizomes of G. uralensis [1]. The G. uralensis ESTs yielded some P450 genes implicated in glycyrrhizin biosynthesis, namely β-amyrin 11-oxidase (CYP88D6) [2]. β-amyrin 11-oxidase (CYP88D6), a cytochrome P450 monooxygenase, has a key role in the biosynthetic pathway of glycyrrhizin. We aimed to study the effect of expression of CYP88D6 on the biosynthesis of glycyrrhizin. A construct harboring cDNA encoding the CYP88D6 gene coupled to the cauliflower mosaic virus (CaMV) 35S promoter and green fluorescent protein (GFP) gene was transformed into G. uralensis hairy roots using Agrobacterium rhizogenes. The transformed hairy root lines were selected by a visual selection of GFP fluorescence. Hairy root line No. 7 had the highest glycyrrhizin content, which was 4.1 times higher than that of control line. Higher levels of CYP88D6 expression were also detected in the hairy root line. We found that a high glycyrrhizin producing hairy root line can be selected, and over-expression of the CYP88D6 gene can cause a marked accumulation of glycyrrhizin and 11-oxo-β-amyrin in the transformed hairy roots.

    Keywords: Glycyrrhiza uralensis, glycyrrhizin, hairy root.

    References:

    [1] Sudo H, Seki H, Sakurai N, Suzuki H, Shibata D, Toyoda A, Totoki Y, Sakaki Y, Iida O, Shibata T, Kojoma M, Muranaka T, Saito K. Expressed sequence tags from rhizomes of Glycyrrhiza uralensis. Plant Biotechnol 2009; 26: 105 – 107

    [2] Seki H, Ohyama K, Sawai S, Mizutami M, Ohnishi T, Sudo H, Akashi T, Aoki T, Saito K, Muranaka T. Licorice β-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin. Proc Natl Acad Sci USA 2008; 105: 14204 – 14209


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