Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596737
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Isolation of bioactive secondary metabolites from mangrove fungal endophytes using epigenetic regulation

SAL Thomas
1   Department of Chemistry
,
R Fleming
2   Cell Biology, Microbiology and Molecular Biology and Center of Drug Discovery and Innovation, 4202 E. Fowler Ave., University of South Florida, Tampa, FL 33620
,
LN Shaw
2   Cell Biology, Microbiology and Molecular Biology and Center of Drug Discovery and Innovation, 4202 E. Fowler Ave., University of South Florida, Tampa, FL 33620
,
BJ Baker
1   Department of Chemistry
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 
 

    Fungal endophytes have gained increasing interest over the past two decades as a source of new bioactive natural products. As it meets a developing source of secondary metabolites, this makes it an ideal focal point for new natural products. Small molecules such as secondary metabolites are targets for potential new drug candidates, especially for antibiotic resistant bacteria. Enterococcus faecium, Staphylococcus aureus, Klebsiella species, Acenitobacter baumanii, Pseudomonas aeruginosa, Enterobacter species (ESKAPE) pathogens are classed as the most infectious diseases because of the lack of effective antibiotics for these pathogens. The need for new drugs can benefit from new methods such as epigenetic regulation of fungal metabolism. In this project, a sample of a black mangrove leaf stem was plated on Sabouraud dextrose agar for isolation of the fungal endophytes. Once isolated it was treated with sodium butyrate, a histone deacetylase (HDAC) inhibitor, resulting in a bioactive extract against methicillin-resistant Staphylococcus aureus at 200 µg/mL. Using bioactivity-guided fractionation the extract went through several stages of separation beginning with normal phase medium-pressure liquid chromatography (MPLC), then using a flash column to further purify the active fraction, which eluted on a gradient of 2% to 12% ethyl acetate in hexane. After obtaining 18 fractions from the flash column, two of the active fractions were purified using high pressure liquid chromatography (HPLC). First, a normal phase preparative column was used followed by a normal phase analytical column. The bioactivity of 200 µg/mL was retained through separation.

    Keywords: Epigenetic regulation, mangrove endophytic fungi.

    References:

    [1] Aly A, Abdessamad D, Julia K, Peter P. Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products. Fungal Diversity Review 2010; 41:1 – 16

    [2] Infectious Diseases Society of America. No ESKAPE! New drugs against MRSA, other superbugs still lacking. Science Daily, 9 December, 2008.


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    No conflict of interest has been declared by the author(s).