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DOI: 10.1055/s-0036-1596462
Zebiriosides A-P, oleanane saponins isolated by LC-SPE-NMR hyphenation from the roots of Dendrobangia boliviana
Publikationsverlauf
Publikationsdatum:
14. Dezember 2016 (online)
A phytochemical study of the roots of Dendrobangia boliviana allowed the identification of twenty saponins, from which sixteen are new structures (Zebiriosides A-P). Fourteen saponins were isolated using classical chromatography techniques (silica gel and RP-18) and six minor saponins (four new and two known) were isolated and identified using hyphenated LC-SPE-NMR system. These saponins are glycosides of serjanic or phytolaccinic acids, whose structures were established on the basis of their spectral data, mainly HR-TOFESIMS, 1D-NMR (1H, 13C, DEPT) and 2D-NMR (1H-1H COSY, TOCSY, HSQC, HMBC, and ROESY), and by comparison with literature data. These compounds were evaluated for their cytotoxic, antileishmanial and hemolytic activities. No antileishmanial or hemolytic activities were revealed, but zebirioside C and zebirioside I showed cytotoxicity against fibroblasts with IC50 of 6.4 and 5.6µM, respectively.
Acknowledgements: Financial support by CNRS, Conseil Regional Champagne Ardenne, Conseil General de la Marne, Ministry of Higher Education and Research (MESR) and EU-programme FEDER to the PlAneT CPER project is gratefully acknowledged.
Keywords: Dendrobangia boliviana, triterpenoid saponins, LC-NMR.
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