RSS-Feed abonnieren
DOI: 10.1055/s-0036-1596312
Chemical profiling by LC-NMR of plants from Peruvian Amazonia with antiparasitic activities
Publikationsverlauf
Publikationsdatum:
14. Dezember 2016 (online)
Malaria and leishmaniasis are considered by the WHO as the first and second most important protozoan vector-borne parasitic diseases in the tropics [1]. Traditional medicine in the Peruvian Amazonian regions is commonly used by local people. Forty-eight plants, without previous phytochemical background, were collected in Allpahuayo National Reserve and Jenaro Herrera community (Loreto – Peru). Based on traditional use, ethanolic extracts were tested in vitro on different parasites strain (Plasmodium falciparum and Leishmania donovani) [2,3]. Piper amazonicum (Miq.) C. DC. (Piperaceae) and Grias neuberthii J.F.Macbr. (Lecythidaceae) showed strong activity (< 1 µg/mL) against Plasmodium falciparum 3D7 chloroquine-sensible strain and < 10 µg/mL against Leishmania donovani (intramacrophagic stage in LV 9 strain), along with low cytotoxicity on macrophages (> 25 µg/mL). In order to determine the anti-malaria mechanism of action, heme binding method by mass spectroscopy (MS) associated to molecular networking interpretation were investigated. Chemical profiling by LC-SPE-NMR of these extracts were performed which allowed the rapid identification of the main active compounds and major known metabolites while avoiding tedious purification procedures [4]. 2 new compounds from P. amazonicum (C13H18N2O6 and C26H16N2O11) and 2 new compounds from G. neuberthii (C37H68N5O6 and C40H79N4O11) are being isolated as the antiplasmodial compounds. The study shows an example of fast targeting of active antiparasitic compounds from small amount of biological material (5 g of plant powder), allowing pharmacological exploration without threatening biodiversity.
Acknowledgements: Consejo Nacional de Ciencia y Tecnología (CONCYTEC) is acknowledged for PhD funding of Pedro Vásquez-Ocmín.
Keywords: Traditional medicine, chemical profiling, malaria, leishmania.
References:
[1] Zofou D, Nyasa RB, Nsagha DS, Ntie-Kang F, Meriki HD, Assob JCN, Kuete V. Control of malaria and other vector-borne protozoan diseases in the tropics: enduring challenges despite considerable progress and achievements. Infect Dis Poverty 2014; 3: 1 – 14
[2] Desjardins RE, Canfield CJ, Haynes JD, Chulay JD. Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother 1979; 16: 710 – 718
[3] Balaraman K, Campos VN, Moussa F, Vacus J, Cojean S, Pomel S, Bories C, Figadère B, Kesavan V, Loiseau P. In vitro and in vivo antileishmanial properties of a 2-n-propylquinoline hydroxypropyl b-cyclodextrin formulation and pharmacokinetics via intravenous route. Biomed Pharmacother 2015; 76: 127 – 133
[4] Hubert J, Nuzillard JM, Renault JH. Dereplication strategies in natural product research: How many tools and methodologies behind the same concept? Phytochem Rev 2015; 1 – 41
#
Die Autoren geben an, dass kein Interessenkonflikt besteht.