Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596294
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Purification of bioactive compounds from Centaurium erythraea by conventional and new generation designed Centrifugal Partition Chromatography column

T Mandova
1   Laboratoire de Pharmacognosie, Université de Paris Descartes, Sorbonne Paris Cité, Faculté de Pharmacie de Paris, UMR-CNRS 8638 COMETE, 4 avenue de l'Observatoire, 75006 Paris, France
,
S Michel
1   Laboratoire de Pharmacognosie, Université de Paris Descartes, Sorbonne Paris Cité, Faculté de Pharmacie de Paris, UMR-CNRS 8638 COMETE, 4 avenue de l'Observatoire, 75006 Paris, France
,
R Grougnet
1   Laboratoire de Pharmacognosie, Université de Paris Descartes, Sorbonne Paris Cité, Faculté de Pharmacie de Paris, UMR-CNRS 8638 COMETE, 4 avenue de l'Observatoire, 75006 Paris, France
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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    Centaurium erythraea Rafn. (Gentianaceae) is a widespread herbaceous plant used in a traditional medicine in more than twenty countries. Hepatoprotective, antibacterial or hypoglycemic activities are reported in the literature. Seco-iridoid glycosides, xanthones, phenolic derivatives and triterpenes were identified as major compounds. In order to recover efficiently the polar constituents, the raw material was macerated in MeOH, EtOH, or EtOH/H2O 1/1, or infused in boiling H2O. UHPLC-MS-UV profiles of each extract enabled to establish the yield in swertiamarin (ST) and sweroside (SR), two structurally closely-related target natural products.

    Centrifugal Partition Chromatography (CPC), also called hydrostatic countercurrent chromatography (HSCCC), a support-free liquid-liquid chromatography based on the partitioning of analytes between two non-miscible liquid phases, was used to isolate ST and SR from the extract. Experimental conditions were optimized on isocratic and gradient mode using a mixture EtOAc/EtOH/H2O on a conventional CPC 250 mL column. These data were then transposed to a new generation 250 mL column. These latter allowed us to perform a five times faster separation compared with the conventional 250 mL column. By gradient run mode, we obtained enriched fraction of each mainly presented seco-iridoids whereof swertiamarin major. The structures of the recovered compounds were unambiguously ascertained by UPLC-ESI-MS/MS and extensive NMR analyses.

    This study has proven the ability of new generation CPC column to purify higher amount of a polar extract in a timeless duration run. The choice of an environment-friendly solvent mixture including the recycling of EtOAc was also noticeable and may contribute to upgrade CPC as a potential industrial production tool.

    Keywords: Seco-iridoids, Centaurium erythraea, centrifugal partition chromatography, counter current chromatography.

    References:

    [1] Goll J, Audo G, Minceva M. Comparison of twin-cell centrifugal partition chromatographic columns with different cell volume J Chromatogr A 2015; 1406: 129 – 135

    [2] Stefkov G, Miova B, Dinevska-Kjovkarovska S, Stanoeva JP, Stefova M, Petrusevska G, Kulevanova S. Chemical characterizationof Centaurium erythrea L. and its effects on carbohydrate and lipid metabolism in experimental diabetes. J Ethnopharmacol 2014; 152: 71 – 77

    [3] Valentao P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. Antioxidant activity of Centaurium erythraea infusion evidenced by its superoxide radical scavenging and xanthine oxidase inhibitory activity. J Agric Food Chem 2001; 49: 3476 – 3479


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    No conflict of interest has been declared by the author(s).