Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596224
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Exploration of the P2X7-receptor pathway involvement in the anti-inflammatory activity of medicinal plants from the European traditional medicine through metabolomics approach

J Zikovic
3   Josif Pancic Institute, Department of Pharmaceutical Research, TadeusaKosuska 11000 Beograd, Serbia
,
G Genta-Jouve
1   Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE
2   Laboratoire de Toxicologie – C-TAC, UMR CNRS 8638 COMETE. Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris
,
F Massicot
2   Laboratoire de Toxicologie – C-TAC, UMR CNRS 8638 COMETE. Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris
,
P Rat
2   Laboratoire de Toxicologie – C-TAC, UMR CNRS 8638 COMETE. Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris
,
B Deguin
1   Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE
,
D Godevac
4   Institute of Chemistry, Technology and Metallurgy, Center for Chemistry, Studentskitrg, 12 – 16, Belgrade, Serbia
,
N Menkovic
3   Josif Pancic Institute, Department of Pharmaceutical Research, TadeusaKosuska 11000 Beograd, Serbia
,
K Savikin
3   Josif Pancic Institute, Department of Pharmaceutical Research, TadeusaKosuska 11000 Beograd, Serbia
,
G Zdunic
3   Josif Pancic Institute, Department of Pharmaceutical Research, TadeusaKosuska 11000 Beograd, Serbia
,
S Boutefnouchet
1   Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

Also available at
 
 

    Use of medicinal plants in folk medicine as anti-inflammatory agents is widespread through Europe, most particularly for chronic arthrosis, migraine...In many cases, this activity is not evidence-based, and the precise mechanism of action and metabolites involved are still unclear. Nevertheless, bibliographic data about two classes of secondary metabolites, sesquiterpenes and iridoids, encouraged us to investigate more precisely plants containing these metabolites. Concerning sesquiterpenes, we focussed in Asteraceae family, whereas for iridoids, species belonging to family of Gentianaceae and Scrophulariaceae were selected. The P2X7 receptor is an interesting tool to investigate anti-inflammatory mechanism as its activation triggers several key inflammatory cytokines including IL-1β, IL-18, TNF-α and IL-6 [1]. Evaluation of both cytotoxicity and P2X7 receptor inhibition was conducted on 31 extracts of selected plants from European folk medicine. Phytochemical analysis using UPLC-MS combined with biostatistical analysis allowed us to identify the panel of metabolites involved in the P2X7 receptor inhibition. Comparison of active versus inactive extracts allowed us to identify 758 metabolites present only in active extracts, among which 34 compounds present in significant concentration range.

    Acknowledgements: Authors thanks the bilateral PHC-PAVLE SAVIC funding and Pr. O. Laprevote for access to UPLC-MS.

    Keywords: Ethnopharmacology, anti-inflammatory, European folk medicine, metabolomic.

    References:

    [1] Lister MF, Sharkey J, Sawatzky DA, Hodgkiss JP, Davidson, DJ, RossiAG, Finlayson KJ. The role of the purinergic P2X7 receptor in inflammation. J Inflamm (Lond) 2007; 1 – 14


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    No conflict of interest has been declared by the author(s).