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Drug Res (Stuttg) 2014; 64(10): 516-522
DOI: 10.1055/s-0033-1363220
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Quantitative Determination of Myricetin in Rat Plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its Absolute Bioavailability

Y. Dang
1   Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
G. Lin
1   Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Y. Xie
1   Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
J. Duan
2   Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
P. Ma
3   Global Pharmaceutical Research and Development, Hospira Inc., McPherson, KS, USA
,
G. Li
4   Pharmacy Department, Shanghai TCM-integrated Hospital, Shanghai, China
,
G. Ji
2   Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China
› Author Affiliations
Further Information

Publication History

received 29 September 2013

accepted 25 November 2013

Publication Date:
19 December 2013 (online)

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Abstract

Myricetin is a widely distributed bioactive flavonoid with scientific interest attributed to its anti-oxidant, antitumor, and anti-inflammatory properties. A specific and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for identification and quantification of myricetin in rat plasma after oral and intravenous administrations. Kaempferol was used as an internal standard. Followed by β-glucuronidase and sulfatase hydrolysis and liquid-liquid extraction with ethyl acetate, the analytes were separated on an Acquity UPLC BEH C18 column (2.1×50 mm, 1.7 μm) and analyzed in the selected ion recording with a negative electrospray ionization mode. The developed method was validated for selectivity, accuracy, precision, linearity, recovery, stability and matrix effect. The assay was validated over a wide concentration range of 2–4 000 ng/mL. Intra- and inter-day precisions were all less than 13.49% and accuracy ranged from 95.75 to 109.80%. The present method was successfully applied to investigate a pharmacokinetic study of myricetin following intravenous and oral administrations to rats. The absolute bioavailability was found to be 9.62% and 9.74% at 2 oral doses (50 mg/kg and 100 mg/kg, respectively), which indicated myricetin was poorly absorbed after oral administration. To our knowledge, this is the first pharmacokinetic evaluation of myricetin as a single active pharmaceutical ingredient in preclinical studies.

Key words

UPLC-MS/MS - myricetin - rat plasma - pharmacokinetics - absolute bioavailability
 

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