Next-Generation-Sequencing in der Immungenetik

Zusammenfassung

Next-Generation-Sequencing ist eine interessante Alternative zu herkömmlichen Verfahren in der hochauflösenden HLA-Typisierung geworden. Insbesondere können mehrdeutige Ergebnisse der Sanger-Sequenzierung aufgelöst werden und eine Vielzahl zusätzlicher Exons getestet werden. Geachtet werden soll aber auf ein robustes System mit möglichst langen Leselängen, die Auswahl der Oliogonukleotide und eine ausreichend hohe Abdeckung der sequenzierten Region.

Abstract

Next generation sequencing is an interesting alternative to existing methods in high resolution HLA-typing. Ambiguous results obtained by Sanger sequencing are resolved and additional exons can be tested. It is important to select a robust system with long reads, to select reliable oligonucleotides and aim at high coverages.

Schlüsselwörter

Ambiguitäten - Next-Generation-Sequencing - Immungenetik - klonale Sequenzierung - Stammzelltransplantation

Key words

ambiguities - next generation sequencing - immunogenetics - stem cell transplantation - clonal sequencing

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