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Planta Med 2013; 79(12): 1031-1037
DOI: 10.1055/s-0032-1328767
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Echinocystic Acid Isolated from Eclipta prostrata Suppresses Lipopolysaccharide-Induced iNOS, TNF-α, and IL-6 Expressions via NF-κB Inactivation in RAW 264.7 Macrophages

Suran Ryu   *
1   Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
2   Department of Biomedical Science, College of Medical Science, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
,
Ji-Sun Shin   *
1   Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
5   Reactive Oxygen Species Medical Research Center, School of Medicine, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
6   Department of Physiology, School of Medicine, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
,
Ji Yun Jung
1   Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
4   Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
,
Young-Wuk Cho
2   Department of Biomedical Science, College of Medical Science, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
5   Reactive Oxygen Species Medical Research Center, School of Medicine, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
6   Department of Physiology, School of Medicine, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
,
Su Jung Kim
3   Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
,
Dae Sik Jang
4   Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
,
Kyung-Tae Lee
1   Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
4   Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 26 November 2012
revised 09 June 2013

accepted 17 June 2013

Publication Date:
22 July 2013 (online)

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Abstract

In this study, we aimed to identify the compounds in Eclipta prostrata responsible for its anti-inflammatory effects using an in vitro bioassay. Three triterpenoids, eclalbasaponin I, eclalbasaponin II, and echinocystic acid, were isolated from an EtOAc fraction of the 70 % EtOH extract of E. prostrata by activity-guided fractionation based on the inhibition of nitric oxide release from lipopolysaccharide-induced RAW 264.7 macrophages. Of these three triterpenoids, echinocystic acid inhibited lipopolysaccharide-induced production of nitric oxide and cytokines such as tumor necrosis factor-α and interleukin-6. Consistent with these observations, echinocystic acid concentration-dependently inhibited lipopolysaccharide-induced inducible nitric oxide synthase expression at the protein level and inducible nitric oxide synthase, tumor necrosis factor-α, and interleukin-6 expression at the mRNA level, and inhibited lipopolysaccharide-induced iNOS promoter binding activity. In addition, echinocystic acid suppressed the lipopolysaccharide-induced transcriptional activity of nuclear factor-κB by blocking the nuclear translocation of p65.

Key words

Eclipta prostrata - Asteraceae - echinocystic acid-nitric oxide - inducible nitric oxide synthase - nuclear factor-κB

* These authors contributed equally to this work.


Supporting Information

 

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