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DOI: 10.1055/s-0032-1317632
An Unusual Diastereoselective Pictet–Spengler Reaction: Synthesis of Novel Tetrahydro-β-Carboline Glycosides
Publication History
Received: 18 August 2012
Accepted after revision: 23 October 2012
Publication Date:
10 December 2012 (online)
Abstract
An unusual kinetic approach to the Pictet–Spengler reaction was investigated, in which l- or d-tryptophan methyl ester reacted with aldehydes of 1,2-O-cyclohexylidene-3-allyloxy-α-d-xylofuranose, yielding exclusively the cis or trans diastereomer of tetrahydro-β-carboline glycoside, respectively, with complete stereocontrol.
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Keywords
Pictet–Spengler reaction - stereoselectivity - diastereoselectivity - stereocontrol - glycosides - imines - π-stacking interactionsThe Pictet–Spengler condensation[ 2 ] is one of the most widely used methods for preparing 1,2,3,4-tetrahydro-β-carbolines and tetrahydroisoquinolines. The reaction has been extensively used for the synthesis of isoquinoline and indole alkaloids[ 3 ] and has been studied both under acidic conditions,[ 4 ] including under microwave irradiation,[ 5 ] and without the aid of an acid or protic solvent.[ 6 ] The importance of this reaction has led organic chemists to focus on the development of stereoselective synthetic routes[ 7 ] that involve either chiral substrates or chiral reagents, including chiral catalysts.
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Here, we report a new methodology in which complete stereocontrol of the Pictet–Spengler conditions results in the formation of 100% cis or trans diastereomeric tetrahydro-β-carboline glycosides from either l- or d-tryptophan methyl ester. The tetrahydro-β-carboline glycosides are important as intermediates in the synthesis of indolo[2,3-a]quinolizine alkaloids[ 8 ] and tetrahydro-β-carboline nucleosides,[ 9 ] which have the ability to bind with DNA or RNA, or as chiral precursors for the stereoselective synthesis of a range of indole alkaloids. The interesting and distinguishable cis and trans stereochemistry of the novel compounds were determined on the basis of 13C NMR spectroscopic analysis, which supports well-documented compression effects.[ 10 ]
In our endeavor to synthesize important heterocyclic intermediates for the synthesis of indolo[2,3-a]quinolizine alkaloids,[ 11 ] tryptamine (1a), or l- or d-tryptophan methyl ester (1b and 1c) was reacted with di(1,2-O-cyclohexylidene-α-d-xylopentodialdofuranose-5-hydrate)-5,5′:3′,5-dianhydride (2a)[ 12 ] (the dimeric form of 1,2-O-cyclohexylidene-3-hydroxy-α-d-xylofuranose-5-carbaldehyde) in dichloromethane with a catalytic amount of trifluoroacetic acid (TFA),[ 13 ] which resulted in the formation of β-carboline glycoside diastereomers 3a and 3b (dr 14:5), 4a and 4b (dr 25:7), or 5a and 5b (dr 7:2), quantitatively. The diastereomeric ratio was calculated on the basis of their isolated yield. When the same Pictet–Spengler reaction was conducted between 1a and 1,2-O-cyclohexylidene-3-allyloxy-α-d-xylofuranose-5-carbaldehyde (2b), or between 1c and 1,2-O-cyclohexylidene-3-propyloxy-α-d-xylofuranose-5-carbaldehyde (2c), the corresponding two diastereomers 6a and 6b (dr 17:7) or 7a and 7b (dr 9:1),[ 14 ] were obtained in quantitative yield (Scheme [1]). Due to the presence of allyl group the diastereomeric 6a shows some improved stereocontrol over 6b.
The interesting stereochemical aspects of the above 1,3-disubstituted β-carboline glycosides were investigated by Cook and co-workers,[ 10 ] who analyzed the 13C NMR spectral data and showed that C-1 and C-3 carbon signals appeared relatively downfield in all the cis isomers in comparison to the trans isomers. They reported that for the β-carboline derivatives obtained when L-tryptophan methyl ester reacted with benzaldehyde, the cis isomer exhibited signals for C-1 and C-3 at δ = 58.7 and 56.9 ppm, respectively, whereas the corresponding signals of the trans isomer appeared at δ = 54.9 and 52.3 ppm. Hence, the hydrogen atoms attached at C-1 and C-3 are on the same face for cis diastereoisomers whereas they are on opposite faces for the trans diastereoisomers. The chemical shifts of C-1 and C-3 carbon atoms of diastereoisomers 4a, 5b and 7b, in our case, appeared at higher shifts in the 13C NMR spectra, and were thus assigned as cis isomers, whereas those of the other diastereomers 4b, 5a and 7a with lower δ values, were assigned as the trans isomers. The 13C NMR chemical shift for C-1 and C-3 of all diastereoisomers are shown in Table [1]. The spatial connectivity were revealed by NOE effects and NOESY correlations between 1-H and 3-H for the diastereoisomer 4a, which confirmed its cis stereochemistry, whereas for 4b there was no such connectivity found, which indicates its trans stereochemistry.
Unusually, 1,2-O-cyclohexylidene-3-allyloxy-α-d-xylofuranose-5-carbaldehyde derivatives 2b and 2d [ 15 ] react with 1b under the same conditions to form only their respective cis diastereomer 8 and 10 (Scheme [2]). Alternatively, they can react with 1c to produce their respective trans diastereomers 9 and 11, exclusively, with more than 98% isolated yield.
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In the mechanism of Pictet–Spengler reaction, the product tetrahydro-β-carboline derivative is obtained through imine formation followed by nucleophilic attack from the 2-position of the indole ring, which is easy because of protonation of the imine in the acidic media. The reason for the observed stereoselectivity using allyl-substituted sugar aldehydes is not yet clear but it is possible that π-stacking interactions between the allyl and the imine intermediate allows cyclization by electrophilic attack on the 2-position of indole through a particular facial orientation. The involvement of a remote allyl group in the mechanism of the reaction that increases the diastereoselectivity of the process is a particularly unusual aspect of this methodology. For propyloxy aldehyde, which is a saturated form of the allyloxy aldehyde that lacks allylic π-electrons, the observed formation of two diastereoisomers supports the above conclusions.
a Determined from 13C NMR spectroscopic analysis of the crude reaction mixture.
On reduction by Pd/C (H2), diastereoisomer 9 gave only diastereomer 7a, confirming that no interconversion between diastereoisomers took place. We also observed that all the major products have the same stereochemistry at C-1, which means that nucleophilic attack during the imine stage favors a particular orientation. For isomers 8–11, the presence of an allyl group and its participation in the π-stacking with the imine occur in that orientation. Molecular model studies of the probable energy-minimized imine intermediate[6a] [16] suggest it may adopt the conformation shown in Figure [1], in which the double-headed arrow indicates possible π-stacking above the plane, and the attack (single-headed bent arrow) takes place from the below the plane.
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In summary, we have developed a straightforward and effective acid-catalyzed synthetic route to tetrahydro-β-carboline glycosides. This process is regioselective and also allows complete control over the stereochemistry at the C-1 and C-3 positions, depending on the substituents present on the 4′-position of the sugar moiety. We believe that π-stacking interactions direct the stereochemistry of the reaction and determines the conformation of the products. The high stereoselectivity exhibited by this methodology will be important for the preparation of a range of indole alkaloid intermediates. Further work on this methodology with other substrates and its application to a broader range of alkaloids towards new drug development is in progress.
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Acknowledgment
The authors thankfully acknowledge CSIR (New Delhi), DRF (Dabur Research Foundation, New Delhi), and the Distance Education Council (DEC; New Delhi) for financial support. The authors are also thankful to Mr. Sudhansu Sekhor Pradhan for his necessary linguistic corrections and to Mr. S. Samaddar for IR spectra.
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References and Notes
- 1 Corresponding author’s current address: Senior Scientist, TCGLS, BN 7, Sector V, Saltlake, Kolkata, 700091, India
- 2 Pictet A, Spengler T. Ber. Dtsch. Chem. Ges. 1911; 44: 2030
- 3 Inorganic reactions . Vol. 6. Whaly WM, Govindachari TR, Adams R. John Wiley & Sons; New York: 1951: 151
- 4a Natarajan S, Ganeshan A. Chem. Commun. 2003; 916
- 4b Yamada H, Kawate T, Matsunigu M, Nishuda A, Yamaguchi K, Nakagawa M. J. Org. Chem. 1998; 63: 6348
- 4c Nikamura S, Tanaka M, Taniguchi T, Uchayama M, Ohwada T. Org. Lett. 2003; 5: 2087
- 5 Jaisankar P, Pal B, Giri VS. Synth. Commun. 2003; 33: 2339
- 6a Soerens D, Sandrin J, Unggemuch F, Mokry P, Wu GS, Yamanaka E, Hutchins L, Dipierro M, Cook JM. J. Org. Chem. 1979; 44: 535
- 6b Cox ED, Cook JM. Chem. Rev. 1995; 95: 1797
- 7a Larghi EL, Marcola A, Bracca AB. J, Kaufman TS. ARKIVOC 2005; (xii): 98
- 7b Pulka K, Kulis P, Tymecka D, Frankiewicz L, Wilczek M, Kozminski W, Misicka A. Tetrahedron 2008; 64: 1506
- 8 Manna RK, Jaisankar P, Giri VS. Synth. Commun. 1995; 25: 3027
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9 Zhao P, Jin H.-W, Yang Z.-J, Zhang L.-R, Zhang L.-H. Org. Biomol. Chem. 2008; 6: 3741
- 10a Sandrin J, Soerens D, Cook JM. Heterocycles 1976; 4: 1249
- 10b Sandrin J, Soerens D, Hutchins L, Richfield E, Ungemach F, Cook JM. Heterocycles 1973; 4: 1101
- 10c Levy GC, Lichter RL, Nelson GL. Carbon-13 Nuclear Magnetic Resonance Spectroscopy. 2nd ed Wiley Interscience; New York: 1980: 55
- 11 Giri VS, Mukherjee R, Burman AC, Gurjar MK, Yadav JS, Jaggi M, Singh AT, Srivastava SK, Jaisankar P, Pal B, Banerjee AK, Wakharkar RD. PCT Int. Appl. WO 2005/121143 A1, 2005 ; Chem. Abstr. 2005: 1330326.
- 12 Furniss BS, Hannaford AJ, Smith PW. G, Tatchell AR. Vogel’s Text Book of Practical Organic Chemistry . Longman Group UK Ltd; England: 1989. 5th ed. 657
- 13 Preparation of Compounds 3–28; General Procedure: To a stirred solution of free tryptamine (1a; 2 mmol) or l-tryptophan methyl ester (1b; 2 mmol) or d-tryptophan methyl ester (1c; 2 mmol), sugar aldehyde 2a–d (2 mmol) and activated 4 Å molecular sieves (10 mg/mmol) in CH2Cl2 (20 mL), TFA (0.2 mL) was added. The reaction mixture was stirred at room temperature for 4–6 h and the progress was monitored by TLC (CHCl3–MeOH, 9:1). Upon completion of the reaction, solvent was removed and the crude material was either directly used for column purification or diluted with H2O, extracted with CH2Cl2 (3 × 25 mL), washed with very dilute aq HCl (10 mL), sat. NaHCO3 (10 mL), H2O (20 mL), and brine (20 mL) and dried over anhydrous Na2SO4 and the solvent was evaporated. The residue was purified by silica-gel column chromatography (petroleum ether–CHCl3–MeOH).10,11-O-Cyclohexylidene-12β-hydroxy-(1-tetrahydro-β-carbonlinyl)tetrahydrofuran (3a) and its conformer (3b): The residue was purified by chromatography over silica gel using CHCl3 to afford 3a (518 mg, 70%) and CHCl3–MeOH (99:1) to afford 3b (188.2 mg, 25%).Compound 3a: mp 200–202 °C; [α]D –54.0 (c 0.48, CHCl3). IR (KBr): 3454, 3088, 1034, 735 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.50 (br s, 1 H, NH), 7.49 (d, J = 7.6 Hz, 1 H), 7.34 (d, J = 7.9 Hz, 1 H), 7.07–7.21 (m, 2 H), 6.08 (d, J = 3.6 Hz, 1 H), 4.53–4.60 (m, 2 H), 4.37–4.41 (m, 1 H), 4.26 (br s, 1 H), 3.33–3.38 (m, 1 H), 2.96–2.99 (m, 2 H), 2.77–2.85 (m, 2 H), 1.40–1.75 (m, 11 H). 13C NMR (75 MHz, CDCl3): δ = 136.0, 131.6, 127.0, 121.8, 119.4, 118.1, 112.5, 111.0, 109.5, 104.9, 85.0, 81.5, 75.6, 52.9, 42.9, 36.4, 35.5, 24.8, 23.9, 23.5, 22.1. MS (ESI): m/z = 371 [M + H]+. Anal. Calcd for C21H26N2O4: C, 68.09; H, 7.07; N, 7.56. Found: C, 67.81; H, 7.37; N, 7.91.Compound 3b: mp 235–237 °C; [α]D –19.7 (c 0.58, CHCl3). IR (KBr): 3454, 2936, 1448, 1120, 1016, 734 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.20 (br s, 1 H, NH), 7.47 (d, J = 7.3 Hz, 1 H), 7.07–7.26 (m, 3 H), 5.98 (d, J = 3.5 Hz, 1 H), 4.49–4.54 (m, 2 H), 4.38 (d, J = 2.5 Hz, 2 H), 3.34–3.43 (m, 1 H), 3.00–3.10 (m, 1 H), 2.64–2.81 (m, 2 H), 1.41–1.74 (m, 11 H). 13C NMR (75 MHz, CDCl3): δ = 136.3, 131.8, 127.7, 122.3, 119.8, 118.5, 112.7, 111.4, 110.6, 105.0, 85.5, 79.3, 77.8, 52.7, 41.9, 37.0, 35.9, 25.2, 24.3, 23.9, 22.3. MS (ESI): m/z = 371 [M + H]+. Anal. Calcd for C21H26N2O4: C, 68.09; H, 7.07; N, 7.56. Found: C, 67.78; H, 7.27; N, 7.51.10,11-O-Cyclohexylidene-12β-hydroxy(1-tetrahydro-3β-carbomethoxycarbolinyl)tetrahydrofuran (4a) and its isomer (4b): The residue was purified by chromatography over silica gel using CHCl3–petroleum ether (95:5) to afford 4a (643 mg, 75%) and the CHCl3 eluent to afford 4b (180 mg, 21%).Compound 4a: mp 90–92 °C; [α]D –50.90 (c 0.5, CHCl3). IR (KBr): 3328, 2937, 1740, 1448, 1072, 753 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.43 (s, 1 H, NH), 7.52 (d, J = 7.7 Hz, 1 H), 7.39 (d, J = 7.9 Hz, 1 H), 7.22 (t, J = 7.9 Hz, 1 H), 7.14 (t, J = 7.5 Hz, 1 H), 6.13 (d, J = 3.4 Hz, 1 H), 4.67 (d, J = 6.4 Hz, 1 H), 4.58 (d, J = 3.4 Hz, 1 H), 4.39 (d, J = 3.4 Hz, 2 H), 3.85 (s, 3 H), 3.78 (t, J = 15.1 Hz, 1 H), 3.22 (dd, J = 15.3, 2.9 Hz, 1 H), 2.89 (t, J = 13.3 Hz, 1 H), 1.27–1.70 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.2, 136.7, 131.9, 127.1, 122.5, 120.0, 118.5, 113.1, 111.6, 108.7, 105.3, 85.3, 82.1, 75.7, 56.2, 53.0, 52.8, 36.8, 35.9, 25.7, 25.2, 24.2, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.51; H, 6.97; N, 6.71.Compound 4b: mp 96–98 °C; [α]D –16.90 (c 0.5, CHCl3). IR (KBr): 3336, 2937, 1737, 1450, 1076, 754 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.56 (s, 1 H, NH), 7.45 (d, J = 7.62 Hz, 1 H), 7.28 (d, J = 7.8 Hz, 1 H), 7.15 (t, J = 7.1 Hz, 1 H), 7.07 (t, J = 7.45 Hz, 1 H), 5.97 (d, J = 3.6 Hz, 1 H), 4.59–4.64 (m, 1 H), 4.53 (d, J = 3.6 Hz, 1 H), 4.34 (t, J = 2.7 Hz, 1 H), 4.28 (t, J = 2.7 Hz, 1 H), 4.16–4.23 (m, 1 H), 3.76 (s, 3 H), 3.13–3.20 (m, 3 H), 2.78–2.81 (m, 1 H), 1.25–1.64 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.9, 136.5, 130.5, 127.1, 122.6, 119.9, 118.4, 112.8, 111.5, 109.1, 104.7, 85.5, 80.4, 76.9, 52.9, 52.6, 51.0, 36.8, 35.9, 25.4, 25.2, 24.2, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.31; H, 7.87; N, 7.01.10,11-O-Cyclohexylidene-12β-hydroxy(1-tetrahydro-3α-carbomethoxy-β-carboli-nyl)tetrahydrofuran (5a) and its isomer (5b): The residue obtained was purified by chromatography over silica gel using CHCl3–petroleum-ether (90:10) eluent to afford 5a (630 mg, 73.5%) and CHCl3 eluent to afford 5b (180 mg, 21%).Compound 5a: mp 142–144 °C; [α]D –44.13 (c 0.5, CHCl3). IR (KBr): 3447, 2934, 1712, 1448, 1022, 739 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.67 (s, 1 H, NH), 7.53 (d, J = 7.4 Hz, 1 H), 7.35 (d, J = 7.9 Hz, 1 H), 7.19 (t, J = 8.8 Hz, 1 H), 7.13 (t, J = 7.6 Hz, 1 H), 6.12 (d, J = 3.3 Hz, 1 H), 4.86 (d, J = 6.6 Hz, 1 H), 4.56 (d, J = 3.3 Hz, 1 H), 4.42 (t, J = 4.5 Hz, 1 H), 4.32 (d, J = 1.6 Hz, 1 H), 4.01 (t, J = 4.8 Hz, 1 H), 3.70 (s, 3 H), 3.12–3.24 (m, 2 H), 1.40–1.71 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 174.2, 136.6, 131.7, 127.1, 122.4, 119.8, 118.5, 113.0, 111.5, 107.5, 105.3, 85.3, 82.1, 75.7, 53.8, 52.7, 49.8, 36.7, 35.9, 25.2, 24.5, 24.3, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.44; H, 7.17; N, 6.82.Compound 5b: mp 120–122 °C; [α]D –10.54 (c 0.2, CHCl3). IR (KBr): 2931, 1728, 1451, 1015, 744 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.33 (s, 1 H, NH), 7.48 (d, J = 7.2 Hz, 1 H), 7.37 (d, J = 8.1 Hz, 1 H), 7.20 (t, J = 8.2 Hz, 1 H), 7.14 (t, J = 8.2 Hz, 1 H), 6.05 (s, 1 H), 4.71 (s, 1 H), 4.55–4.66 (m, 2 H), 4.53 (s, 1 H), 3.84 (s, 3 H), 3.64 (dd, J = 11.2, 2.9 Hz, 1 H), 2.99 (t, J = 12.6 Hz, 1 H), 2.93 (t, J = 11.4 Hz, 1 H), 1.45–1.85 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.0, 136.6, 131.6, 127.3, 122.7, 120.2, 118.4, 112.9, 111.6, 109.2, 105.1, 85.2, 80.0, 76.9, 56.5, 53.3, 52.7, 37.0, 35.9, 25.8, 25.2, 24.3, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.31; H, 7.07; N, 6.61.10,11-O-Cyclohexylidene-12β-allyloxy(1-tetrahydro-β-carbolinyl)tetrahydrofuran (6a) and its isomer (6b): The residue was purified by chromatography over silica gel using CHCl3 to afford 6a (558 mg, 68%) and CHCl3–MeOH (99:1) eluent to afford 6b (229.8 mg, 28%).Compound 6a: mp 56–58 °C; [α]D –107.72 (c 0.1, CHCl3). IR (KBr): 3449, 2935, 1449, 1163, 1022, 741 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.75 (s, 1 H, NH), 7.53 (d, J = 7.4 Hz, 1 H), 7.39 (d, J = 7.8 Hz, 1 H), 7.18 (t, J = 7.1 Hz, 1 H), 7.14 (t, J = 6.3 Hz, 1 H), 6.11 (d, J = 3.6 Hz, 1 H), 5.96 (m, 1 H), 5.20–5.36 (m, 2 H), 4.70–4.72 (d, J = 3.6 Hz, 1 H), 4.32–4.37 (m, 2 H), 4.06–4.18 (m, 3 H), 3.12–3.26 (m, 2 H), 2.71–2.84 (m, 2 H), 2.62 (br s, 1 H, NH), 1.25–1.75 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 136.2, 133.2, 134.4, 127.0, 122.0, 119.3, 118.4, 117.9, 113.6, 111.5, 109.2, 106.3, 88.0, 84.4, 84.3, 71.9, 52.9, 41.7, 36.6, 35.3, 25.2, 24.2, 23.8, 22.7. MS (ESI): m/z = 433 [M + Na]+. Anal. Calcd for C24H30N2O4: C, 70.22; H, 7.37; N, 6.82. Found: C, 70.12; H, 7.27; N, 6.92.Compound 6b: mp 58–60 °C; [α]D –111.28 (c 0.1, CHCl3). IR (KBr): 3443, 2935, 1449, 1113, 1018, 744 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.78 (s, 1 H, NH), 7.53 (d, J = 7.4 Hz, 1 H), 7.34 (d, J = 7.7 Hz, 1 H), 7.12 (t, J = 7.0 Hz, 1 H), 6.17 (t, J = 6.8 Hz, 1 H), 5.96–6.03 (m, 2 H), 5.12–5.42 (m, 3 H), 4.63 (d, J = 3.7 Hz, 1 H), 4.15–4.39 (m, 4 H), 2.50–3.42 (m, 5 H including NH), 1.25–1.85 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 136.1, 134.4, 134.1, 127.4, 119.4, 118.6, 118.3, 113.2, 112.8, 111.5, 109.0, 105.3, 83.8, 82.1, 81.7, 71.3, 51.6, 43.6, 36.7, 36.3, 25.2, 24.2, 24.0, 22.6. MS (ESI): m/z = 433 [M + Na]+. Anal. Calcd for C24H30N2O4: C, 70.22; H, 7.37; N, 6.82. Found: C, 69.72; H, 7.47; N, 7.02.10,11-O-Cyclohexylidene-12β-propyloxy(1-tetrahydro-3α-carbomethoxy-β-carbolinyl)tetrahydrofuran (7a) and its isomer (7b): The residue was purified by chromatography over silica gel using CHCl3–petroleum-ether (90:10) eluent to afford 7a (757.5 mg, 80.5%) first and then 7b (108.2 mg, 11.5%).Compound 7a: mp 170–174 °C; [α]D –175.04 (c 0.1, CHCl3). IR (neat): 3449, 2936, 1737, 1451, 1115, 746 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.60 (s, 1 H, NH), 7.52 (d, J = 7.7 Hz, 1 H), 7.36 (d, J = 7.8 Hz, 1 H), 7.19 (t, J = 7.8 Hz, 1 H), 7.12 (t, J = 7.5 Hz, 1 H), 6.08 (d, J = 3.4 Hz, 1 H), 4.66 (m, 2 H), 4.27 (m, 1 H), 4.11 (m, 1 H), 4.03 (m, 1 H), 3.72 (m, 4 H), 3.59 (m, 1 H), 3.18 (m, 2 H), 1.24–1.77 (m, 12 H), 1.06–1.02 (m, 3 H). 13C NMR (75 MHz, CDCl3): δ = 173.8, 135.9, 133.0, 126.6, 121.5, 118.9, 117.9, 112.6, 111.0, 106.5, 105.0, 83.4, 82.4, 81.2, 72.0, 53.8, 52.0, 48.3, 36.3, 35.8, 24.7, 24.0, 23.7, 23.5, 23.0, 10.5. MS (ESI): m/z = 493 [M + Na]+. Anal. Calcd for C26H34N2O6: C, 66.36; H, 7.28; N, 5.95. Found: C, 66.46; H, 7.37; N, 6.08.Compound 7b: 13C NMR (75 MHz, CDCl3; obtained from a mixture with 7a): δ = 173.1, 136.1, 132.2, 127.1, 121.7, 119.4, 117.7, 112.5, 110.8, 109.0, 104.2, 83.6, 83.1, 81.4, 72.4, 56.2, 53.9, 52.5, 36.5, 35.7, 25.2, 24.2, 23.9, 23.4, 23.1, 10.5.10,11-O-Cyclohexylidene-12β-allyloxy(1-tetrahydro-3β-carbomethoxy-β-carboli-nyl)tetrahydrofuran (8) and 10,11-O-Cyclohexylidene-12β-allyloxy(1-tetrahydro-3α-carbomethoxy-β-carbolinyl)tetrahydrofuran (9): The residue obtained was purified by chromatography over silica gel using CHCl3–petroleum-ether (90:10) eluent to afford 8 (915 mg, 97.5%) or 9 (919 mg, 98%), respectively.Compound 8: mp 62–64 °C; [α]D –142.36 (c 0.1, CHCl3). IR (KBr): 3448, 2935, 1738, 1448, 1165, 1023, 742 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.80 (s, 1 H, NH), 7.55 (d, J = 7.51 Hz, 1 H), 7.41 (d, J = 7.82 Hz, 1 H), 7.20 (t, J = 14.1 Hz, 1 H), 7.14 (t, J = 14.2 Hz, 1 H), 6.13–6.15 (m, 2 H), 5.36–5.48 (m, 2 H), 4.69 (d, J = 3.66 Hz, 1 H), 4.52 (d, J = 8.61 Hz, 2 H), 4.35 (dd, J = 5.22, 13.13 Hz, 2 H), 4.25 (d, J = 2.90 Hz, 1 H), 4.13 (dd, J = 12.6, 6.5 Hz, 1 H), 3.87 (s, 3 H), 3.25 (dd, J = 14.9, 2.9 Hz, 1 H), 2.95 (t, J = 15.8 Hz, 1 H), 2.60 (br s, 1 H, NH), 1.32–1.73 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.5, 136.5, 134.3, 133.8, 127.1, 122.0, 119.6, 119.4, 118.3, 113.3, 111.6, 107.9, 105.5, 83.9, 81.9, 81.7, 71.3, 56.5, 52.5, 52.3, 36.8, 36.4, 25.5, 25.2, 24.2, 24.0. MS (ESI): m/z = 491 [M + Na]+. Anal. Calcd for C26H32N2O6: C, 66.65; H, 6.88; N, 5.98. Found: C, 66.72; H, 7.07; N, 5.91.Compound 9: mp 60–62 °C; [α]D –147.16 (c 0.1, CHCl3). IR (KBr): 3446, 2934, 1736, 1451, 1164, 1021, 746 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.60 (s, 1 H, NH), 7.53 (d, J = 7.30 Hz, 1 H), 7.37 (d, J = 7.72 Hz, 1 H), 7.18 (t, J = 7.40 Hz, 1 H), 7.11 (t, J = 7.34 Hz, 1 H), 6.01–6.09 (m, 2 H), 5.46 (d, J = 17.20 Hz, 1 H), 5.32 (d, J = 10.46 Hz, 1 H), 4.67 (t, J = 19.8 Hz, 2 H), 4.25–4.36 (m, 2 H), 4.08–4.17 (m, 2 H), 4.01 (s, 1 H), 3.73 (s, 3 H), 3.17 (d, J = 3.61 Hz, 2 H), 2.56 (br s, 1 H, NH), 1.29–1.60 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 174.3, 136.4, 134.1, 133.6, 127.1, 122.0, 119.4, 118.6, 118.3, 113.1, 111.5, 107.1, 105.4, 83.8, 82.1, 81.7, 71.4, 54.2, 52.4, 48.6, 36.7, 36.2, 25.2, 24.6, 24.2, 24.0. MS (ESI): m/z = 491 [M + Na]+. Anal. Calcd for C26H32N2O6: C, 66.65; H, 6.88; N, 5.98. Found: C, 65.92; H, 7.27; N, 6.01.4,5-O-cyclohexyl-3-(2-methylallyloxy)tetrahydrofuran-2-carbaldehyde (2c): Colorless syrup; [α]D 18.9 –54.614 (c 1 mM, CHCl3). IR (neat): 1737, 1672, 1168, 1022, 738 cm–1. 1H NMR (600 MHz, CDCl3): δ = 9.62 (d, J = 2.1 Hz, 1 H), 6.07 (d, J = 3.42 Hz, 1 H), 4.86 (d, J = 8.94 Hz, 2 H), 4.55 (d, J = 3.42 Hz, 1 H), 4.49 (d, J = 3.42 Hz, 1 H), 4.22 (d, J = 4.14 Hz, 1 H), 3.91 (d, J = 12.36 Hz, 1 H), 3.79 (d, J = 12.42 Hz, 1 H), 1.61 (s, 3 H), 1.40–1.60 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 200.2, 140.8, 113.5, 113.3, 105.9, 84.6, 84.0, 81.7, 72.0, 36.7, 35.9, 24.8, 23.8, 23.6, 19.3. MS (ESI): m/z = 305 [M + Na]+. Anal. Calcd for C15H22O5: C, 63.81; H, 7.85. Found: C, 63.77; H, 7.91.10,11-O-Cyclohexylidene-12β-(2-methylallyloxy)-(1-tetrahydro-3β-carbomethoxy-β-carboli-nyl)tetrahydro-furan (10) and 10,11-O-Cyclohexylidene-12β-(2-methyl-allyloxy)-(1-tetrahydro-3α-carbomethoxy-β-carbolinyl)-tetrahydrofuran (10): The residue obtained was purified by chromatography over silica gel using CHCl3–MeOH (99:1) eluent to afford 10 (950 mg, 98.4%) or 11 (948 mg, 98.2%), respectively.Compound 10: mp 174–176 °C; [α]D 20.5 –41.29 (c 1 mM, CHCl3). IR (KBr): 3452, 2933, 1739, 1448, 1165, 1025, 741 cm–1. 1H NMR (600 MHz, CDCl3): δ = 8.75 (br s, 1 H, NH), 7.21–7.45 (m, 2 H), 7.05 (t, J = 9.8 Hz, 1 H), 6.97 (t, J = 9.6 Hz, 1 H), 5.82 (s, 1 H), 4.85–5.25 (m, 2 H), 4.67 (s, 2 H), 4.25–4.55 (m, 3 H), 3.80–4.15 (m, 2 H), 3.70 (m, 1 H), 3.55 (s, 3 H), 3.23 (m, 1 H), 2.84 (br s, 1 H), 1.10–1.70 (m, 13 H). 13C NMR (600 MHz, CDCl3): δ = 172.5, 140.2, 137.0, 136.9, 125.9, 122.3, 119.4, 117.9, 114.1, 113.3, 111.9, 107.1, 104.6, 83.2, 81.1, 80.9, 74.2, 57.9, 56.7, 52.3, 36.1, 35.7, 24.7, 23.6, 23.5, 23.4, 19.3. MS (ESI): m/z = 506 [M + Na]+. Anal. Calcd for C27H34N2O6: C, 67.20; H, 7.10; N, 5.81. Found: C, 67.31; H, 7.11; N, 5.79.Compound 11: mp 166–168 °C; [α]D 19.5 –37.35 (c 1 mM, CHCl3). IR (KBr): 3445, 2932, 1739, 1450, 1167, 1021, 747 cm–1. 1H NMR (600 MHz, CDCl3): δ = 8.73 (br s, 1 H, NH), 7.40 (t, J = 8.94 Hz, 1 H), 7.20 (t, J = 4.8 Hz, 1 H), 7.09 (t, J = 7.56 Hz, 1 H), 7.02 (dd, J = 7.56 Hz, 1 H), 5.81 (s, 1 H), 4.80–5.10 (m, 2 H), 4.45–4.65 (m, 3 H), 4.00–4.20 (m, 2 H), 3.75–3.90 (m, 2 H), 3.45 (s, 3 H), 3.10 (s, 2 H), 2.98 (s, 1 H, NH), 1.63 (s, 3 H), 1.10–1.60 (m, 10 H). 13C NMR (600 MHz, CDCl3): δ = 172.1, 141.0, 136.9, 136.4, 126.1, 122.5, 119.7, 118.4, 113.5, 113.2, 111.6, 104.7, 104.4, 83.4, 81.3, 81.1, 73.8, 54.5, 53.0, 50.5, 36.2, 35.8, 24.7, 23.8, 23.6 (2), 19.7. MS (ESI): m/z = 506 [M + Na]+. Anal. Calcd for C27H34N2O6: C, 67.20; H, 7.10; N, 5.81. Found: C, 67.21; H, 7.13; N, 5.75
- 14 It was not possible to isolate the minor diastereoisomer, which was formed as an inseparable mixture with the major diastereoisomer. Hence, the diastereomeric ratio (dr) was determined from LCMS retention time and peak area of the diastereoisomers in crude reaction mixture
- 15a Sharma GV. M, Begum A, Reddy KR, Sankar AR, Kunwar AC. Tetrahedron: Asymmetry 2003; 14: 3899
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15b Smith AB, Rivero RA, Hale KJ, Vaccaro HA. J. Am. Chem. Soc. 1991; 113: 2092
- 15c Bhattacharjee A, Datta S, Chattopadhyay P, Ghoshal N, Kundu AP, Pal A, Mukhopadhyay R, Chowdhury S, Bhattacharjya A, Patra A. Tetrahedron 2003; 59: 4623
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References and Notes
- 1 Corresponding author’s current address: Senior Scientist, TCGLS, BN 7, Sector V, Saltlake, Kolkata, 700091, India
- 2 Pictet A, Spengler T. Ber. Dtsch. Chem. Ges. 1911; 44: 2030
- 3 Inorganic reactions . Vol. 6. Whaly WM, Govindachari TR, Adams R. John Wiley & Sons; New York: 1951: 151
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- 4c Nikamura S, Tanaka M, Taniguchi T, Uchayama M, Ohwada T. Org. Lett. 2003; 5: 2087
- 5 Jaisankar P, Pal B, Giri VS. Synth. Commun. 2003; 33: 2339
- 6a Soerens D, Sandrin J, Unggemuch F, Mokry P, Wu GS, Yamanaka E, Hutchins L, Dipierro M, Cook JM. J. Org. Chem. 1979; 44: 535
- 6b Cox ED, Cook JM. Chem. Rev. 1995; 95: 1797
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- 7b Pulka K, Kulis P, Tymecka D, Frankiewicz L, Wilczek M, Kozminski W, Misicka A. Tetrahedron 2008; 64: 1506
- 8 Manna RK, Jaisankar P, Giri VS. Synth. Commun. 1995; 25: 3027
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9 Zhao P, Jin H.-W, Yang Z.-J, Zhang L.-R, Zhang L.-H. Org. Biomol. Chem. 2008; 6: 3741
- 10a Sandrin J, Soerens D, Cook JM. Heterocycles 1976; 4: 1249
- 10b Sandrin J, Soerens D, Hutchins L, Richfield E, Ungemach F, Cook JM. Heterocycles 1973; 4: 1101
- 10c Levy GC, Lichter RL, Nelson GL. Carbon-13 Nuclear Magnetic Resonance Spectroscopy. 2nd ed Wiley Interscience; New York: 1980: 55
- 11 Giri VS, Mukherjee R, Burman AC, Gurjar MK, Yadav JS, Jaggi M, Singh AT, Srivastava SK, Jaisankar P, Pal B, Banerjee AK, Wakharkar RD. PCT Int. Appl. WO 2005/121143 A1, 2005 ; Chem. Abstr. 2005: 1330326.
- 12 Furniss BS, Hannaford AJ, Smith PW. G, Tatchell AR. Vogel’s Text Book of Practical Organic Chemistry . Longman Group UK Ltd; England: 1989. 5th ed. 657
- 13 Preparation of Compounds 3–28; General Procedure: To a stirred solution of free tryptamine (1a; 2 mmol) or l-tryptophan methyl ester (1b; 2 mmol) or d-tryptophan methyl ester (1c; 2 mmol), sugar aldehyde 2a–d (2 mmol) and activated 4 Å molecular sieves (10 mg/mmol) in CH2Cl2 (20 mL), TFA (0.2 mL) was added. The reaction mixture was stirred at room temperature for 4–6 h and the progress was monitored by TLC (CHCl3–MeOH, 9:1). Upon completion of the reaction, solvent was removed and the crude material was either directly used for column purification or diluted with H2O, extracted with CH2Cl2 (3 × 25 mL), washed with very dilute aq HCl (10 mL), sat. NaHCO3 (10 mL), H2O (20 mL), and brine (20 mL) and dried over anhydrous Na2SO4 and the solvent was evaporated. The residue was purified by silica-gel column chromatography (petroleum ether–CHCl3–MeOH).10,11-O-Cyclohexylidene-12β-hydroxy-(1-tetrahydro-β-carbonlinyl)tetrahydrofuran (3a) and its conformer (3b): The residue was purified by chromatography over silica gel using CHCl3 to afford 3a (518 mg, 70%) and CHCl3–MeOH (99:1) to afford 3b (188.2 mg, 25%).Compound 3a: mp 200–202 °C; [α]D –54.0 (c 0.48, CHCl3). IR (KBr): 3454, 3088, 1034, 735 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.50 (br s, 1 H, NH), 7.49 (d, J = 7.6 Hz, 1 H), 7.34 (d, J = 7.9 Hz, 1 H), 7.07–7.21 (m, 2 H), 6.08 (d, J = 3.6 Hz, 1 H), 4.53–4.60 (m, 2 H), 4.37–4.41 (m, 1 H), 4.26 (br s, 1 H), 3.33–3.38 (m, 1 H), 2.96–2.99 (m, 2 H), 2.77–2.85 (m, 2 H), 1.40–1.75 (m, 11 H). 13C NMR (75 MHz, CDCl3): δ = 136.0, 131.6, 127.0, 121.8, 119.4, 118.1, 112.5, 111.0, 109.5, 104.9, 85.0, 81.5, 75.6, 52.9, 42.9, 36.4, 35.5, 24.8, 23.9, 23.5, 22.1. MS (ESI): m/z = 371 [M + H]+. Anal. Calcd for C21H26N2O4: C, 68.09; H, 7.07; N, 7.56. Found: C, 67.81; H, 7.37; N, 7.91.Compound 3b: mp 235–237 °C; [α]D –19.7 (c 0.58, CHCl3). IR (KBr): 3454, 2936, 1448, 1120, 1016, 734 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.20 (br s, 1 H, NH), 7.47 (d, J = 7.3 Hz, 1 H), 7.07–7.26 (m, 3 H), 5.98 (d, J = 3.5 Hz, 1 H), 4.49–4.54 (m, 2 H), 4.38 (d, J = 2.5 Hz, 2 H), 3.34–3.43 (m, 1 H), 3.00–3.10 (m, 1 H), 2.64–2.81 (m, 2 H), 1.41–1.74 (m, 11 H). 13C NMR (75 MHz, CDCl3): δ = 136.3, 131.8, 127.7, 122.3, 119.8, 118.5, 112.7, 111.4, 110.6, 105.0, 85.5, 79.3, 77.8, 52.7, 41.9, 37.0, 35.9, 25.2, 24.3, 23.9, 22.3. MS (ESI): m/z = 371 [M + H]+. Anal. Calcd for C21H26N2O4: C, 68.09; H, 7.07; N, 7.56. Found: C, 67.78; H, 7.27; N, 7.51.10,11-O-Cyclohexylidene-12β-hydroxy(1-tetrahydro-3β-carbomethoxycarbolinyl)tetrahydrofuran (4a) and its isomer (4b): The residue was purified by chromatography over silica gel using CHCl3–petroleum ether (95:5) to afford 4a (643 mg, 75%) and the CHCl3 eluent to afford 4b (180 mg, 21%).Compound 4a: mp 90–92 °C; [α]D –50.90 (c 0.5, CHCl3). IR (KBr): 3328, 2937, 1740, 1448, 1072, 753 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.43 (s, 1 H, NH), 7.52 (d, J = 7.7 Hz, 1 H), 7.39 (d, J = 7.9 Hz, 1 H), 7.22 (t, J = 7.9 Hz, 1 H), 7.14 (t, J = 7.5 Hz, 1 H), 6.13 (d, J = 3.4 Hz, 1 H), 4.67 (d, J = 6.4 Hz, 1 H), 4.58 (d, J = 3.4 Hz, 1 H), 4.39 (d, J = 3.4 Hz, 2 H), 3.85 (s, 3 H), 3.78 (t, J = 15.1 Hz, 1 H), 3.22 (dd, J = 15.3, 2.9 Hz, 1 H), 2.89 (t, J = 13.3 Hz, 1 H), 1.27–1.70 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.2, 136.7, 131.9, 127.1, 122.5, 120.0, 118.5, 113.1, 111.6, 108.7, 105.3, 85.3, 82.1, 75.7, 56.2, 53.0, 52.8, 36.8, 35.9, 25.7, 25.2, 24.2, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.51; H, 6.97; N, 6.71.Compound 4b: mp 96–98 °C; [α]D –16.90 (c 0.5, CHCl3). IR (KBr): 3336, 2937, 1737, 1450, 1076, 754 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.56 (s, 1 H, NH), 7.45 (d, J = 7.62 Hz, 1 H), 7.28 (d, J = 7.8 Hz, 1 H), 7.15 (t, J = 7.1 Hz, 1 H), 7.07 (t, J = 7.45 Hz, 1 H), 5.97 (d, J = 3.6 Hz, 1 H), 4.59–4.64 (m, 1 H), 4.53 (d, J = 3.6 Hz, 1 H), 4.34 (t, J = 2.7 Hz, 1 H), 4.28 (t, J = 2.7 Hz, 1 H), 4.16–4.23 (m, 1 H), 3.76 (s, 3 H), 3.13–3.20 (m, 3 H), 2.78–2.81 (m, 1 H), 1.25–1.64 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.9, 136.5, 130.5, 127.1, 122.6, 119.9, 118.4, 112.8, 111.5, 109.1, 104.7, 85.5, 80.4, 76.9, 52.9, 52.6, 51.0, 36.8, 35.9, 25.4, 25.2, 24.2, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.31; H, 7.87; N, 7.01.10,11-O-Cyclohexylidene-12β-hydroxy(1-tetrahydro-3α-carbomethoxy-β-carboli-nyl)tetrahydrofuran (5a) and its isomer (5b): The residue obtained was purified by chromatography over silica gel using CHCl3–petroleum-ether (90:10) eluent to afford 5a (630 mg, 73.5%) and CHCl3 eluent to afford 5b (180 mg, 21%).Compound 5a: mp 142–144 °C; [α]D –44.13 (c 0.5, CHCl3). IR (KBr): 3447, 2934, 1712, 1448, 1022, 739 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.67 (s, 1 H, NH), 7.53 (d, J = 7.4 Hz, 1 H), 7.35 (d, J = 7.9 Hz, 1 H), 7.19 (t, J = 8.8 Hz, 1 H), 7.13 (t, J = 7.6 Hz, 1 H), 6.12 (d, J = 3.3 Hz, 1 H), 4.86 (d, J = 6.6 Hz, 1 H), 4.56 (d, J = 3.3 Hz, 1 H), 4.42 (t, J = 4.5 Hz, 1 H), 4.32 (d, J = 1.6 Hz, 1 H), 4.01 (t, J = 4.8 Hz, 1 H), 3.70 (s, 3 H), 3.12–3.24 (m, 2 H), 1.40–1.71 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 174.2, 136.6, 131.7, 127.1, 122.4, 119.8, 118.5, 113.0, 111.5, 107.5, 105.3, 85.3, 82.1, 75.7, 53.8, 52.7, 49.8, 36.7, 35.9, 25.2, 24.5, 24.3, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.44; H, 7.17; N, 6.82.Compound 5b: mp 120–122 °C; [α]D –10.54 (c 0.2, CHCl3). IR (KBr): 2931, 1728, 1451, 1015, 744 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.33 (s, 1 H, NH), 7.48 (d, J = 7.2 Hz, 1 H), 7.37 (d, J = 8.1 Hz, 1 H), 7.20 (t, J = 8.2 Hz, 1 H), 7.14 (t, J = 8.2 Hz, 1 H), 6.05 (s, 1 H), 4.71 (s, 1 H), 4.55–4.66 (m, 2 H), 4.53 (s, 1 H), 3.84 (s, 3 H), 3.64 (dd, J = 11.2, 2.9 Hz, 1 H), 2.99 (t, J = 12.6 Hz, 1 H), 2.93 (t, J = 11.4 Hz, 1 H), 1.45–1.85 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.0, 136.6, 131.6, 127.3, 122.7, 120.2, 118.4, 112.9, 111.6, 109.2, 105.1, 85.2, 80.0, 76.9, 56.5, 53.3, 52.7, 37.0, 35.9, 25.8, 25.2, 24.3, 23.9. MS (ESI): m/z = 429 [M + H]+. Anal. Calcd for C23H28N2O6: C, 64.47; H, 6.59; N, 6.54. Found: C, 64.31; H, 7.07; N, 6.61.10,11-O-Cyclohexylidene-12β-allyloxy(1-tetrahydro-β-carbolinyl)tetrahydrofuran (6a) and its isomer (6b): The residue was purified by chromatography over silica gel using CHCl3 to afford 6a (558 mg, 68%) and CHCl3–MeOH (99:1) eluent to afford 6b (229.8 mg, 28%).Compound 6a: mp 56–58 °C; [α]D –107.72 (c 0.1, CHCl3). IR (KBr): 3449, 2935, 1449, 1163, 1022, 741 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.75 (s, 1 H, NH), 7.53 (d, J = 7.4 Hz, 1 H), 7.39 (d, J = 7.8 Hz, 1 H), 7.18 (t, J = 7.1 Hz, 1 H), 7.14 (t, J = 6.3 Hz, 1 H), 6.11 (d, J = 3.6 Hz, 1 H), 5.96 (m, 1 H), 5.20–5.36 (m, 2 H), 4.70–4.72 (d, J = 3.6 Hz, 1 H), 4.32–4.37 (m, 2 H), 4.06–4.18 (m, 3 H), 3.12–3.26 (m, 2 H), 2.71–2.84 (m, 2 H), 2.62 (br s, 1 H, NH), 1.25–1.75 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 136.2, 133.2, 134.4, 127.0, 122.0, 119.3, 118.4, 117.9, 113.6, 111.5, 109.2, 106.3, 88.0, 84.4, 84.3, 71.9, 52.9, 41.7, 36.6, 35.3, 25.2, 24.2, 23.8, 22.7. MS (ESI): m/z = 433 [M + Na]+. Anal. Calcd for C24H30N2O4: C, 70.22; H, 7.37; N, 6.82. Found: C, 70.12; H, 7.27; N, 6.92.Compound 6b: mp 58–60 °C; [α]D –111.28 (c 0.1, CHCl3). IR (KBr): 3443, 2935, 1449, 1113, 1018, 744 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.78 (s, 1 H, NH), 7.53 (d, J = 7.4 Hz, 1 H), 7.34 (d, J = 7.7 Hz, 1 H), 7.12 (t, J = 7.0 Hz, 1 H), 6.17 (t, J = 6.8 Hz, 1 H), 5.96–6.03 (m, 2 H), 5.12–5.42 (m, 3 H), 4.63 (d, J = 3.7 Hz, 1 H), 4.15–4.39 (m, 4 H), 2.50–3.42 (m, 5 H including NH), 1.25–1.85 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 136.1, 134.4, 134.1, 127.4, 119.4, 118.6, 118.3, 113.2, 112.8, 111.5, 109.0, 105.3, 83.8, 82.1, 81.7, 71.3, 51.6, 43.6, 36.7, 36.3, 25.2, 24.2, 24.0, 22.6. MS (ESI): m/z = 433 [M + Na]+. Anal. Calcd for C24H30N2O4: C, 70.22; H, 7.37; N, 6.82. Found: C, 69.72; H, 7.47; N, 7.02.10,11-O-Cyclohexylidene-12β-propyloxy(1-tetrahydro-3α-carbomethoxy-β-carbolinyl)tetrahydrofuran (7a) and its isomer (7b): The residue was purified by chromatography over silica gel using CHCl3–petroleum-ether (90:10) eluent to afford 7a (757.5 mg, 80.5%) first and then 7b (108.2 mg, 11.5%).Compound 7a: mp 170–174 °C; [α]D –175.04 (c 0.1, CHCl3). IR (neat): 3449, 2936, 1737, 1451, 1115, 746 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.60 (s, 1 H, NH), 7.52 (d, J = 7.7 Hz, 1 H), 7.36 (d, J = 7.8 Hz, 1 H), 7.19 (t, J = 7.8 Hz, 1 H), 7.12 (t, J = 7.5 Hz, 1 H), 6.08 (d, J = 3.4 Hz, 1 H), 4.66 (m, 2 H), 4.27 (m, 1 H), 4.11 (m, 1 H), 4.03 (m, 1 H), 3.72 (m, 4 H), 3.59 (m, 1 H), 3.18 (m, 2 H), 1.24–1.77 (m, 12 H), 1.06–1.02 (m, 3 H). 13C NMR (75 MHz, CDCl3): δ = 173.8, 135.9, 133.0, 126.6, 121.5, 118.9, 117.9, 112.6, 111.0, 106.5, 105.0, 83.4, 82.4, 81.2, 72.0, 53.8, 52.0, 48.3, 36.3, 35.8, 24.7, 24.0, 23.7, 23.5, 23.0, 10.5. MS (ESI): m/z = 493 [M + Na]+. Anal. Calcd for C26H34N2O6: C, 66.36; H, 7.28; N, 5.95. Found: C, 66.46; H, 7.37; N, 6.08.Compound 7b: 13C NMR (75 MHz, CDCl3; obtained from a mixture with 7a): δ = 173.1, 136.1, 132.2, 127.1, 121.7, 119.4, 117.7, 112.5, 110.8, 109.0, 104.2, 83.6, 83.1, 81.4, 72.4, 56.2, 53.9, 52.5, 36.5, 35.7, 25.2, 24.2, 23.9, 23.4, 23.1, 10.5.10,11-O-Cyclohexylidene-12β-allyloxy(1-tetrahydro-3β-carbomethoxy-β-carboli-nyl)tetrahydrofuran (8) and 10,11-O-Cyclohexylidene-12β-allyloxy(1-tetrahydro-3α-carbomethoxy-β-carbolinyl)tetrahydrofuran (9): The residue obtained was purified by chromatography over silica gel using CHCl3–petroleum-ether (90:10) eluent to afford 8 (915 mg, 97.5%) or 9 (919 mg, 98%), respectively.Compound 8: mp 62–64 °C; [α]D –142.36 (c 0.1, CHCl3). IR (KBr): 3448, 2935, 1738, 1448, 1165, 1023, 742 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.80 (s, 1 H, NH), 7.55 (d, J = 7.51 Hz, 1 H), 7.41 (d, J = 7.82 Hz, 1 H), 7.20 (t, J = 14.1 Hz, 1 H), 7.14 (t, J = 14.2 Hz, 1 H), 6.13–6.15 (m, 2 H), 5.36–5.48 (m, 2 H), 4.69 (d, J = 3.66 Hz, 1 H), 4.52 (d, J = 8.61 Hz, 2 H), 4.35 (dd, J = 5.22, 13.13 Hz, 2 H), 4.25 (d, J = 2.90 Hz, 1 H), 4.13 (dd, J = 12.6, 6.5 Hz, 1 H), 3.87 (s, 3 H), 3.25 (dd, J = 14.9, 2.9 Hz, 1 H), 2.95 (t, J = 15.8 Hz, 1 H), 2.60 (br s, 1 H, NH), 1.32–1.73 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 173.5, 136.5, 134.3, 133.8, 127.1, 122.0, 119.6, 119.4, 118.3, 113.3, 111.6, 107.9, 105.5, 83.9, 81.9, 81.7, 71.3, 56.5, 52.5, 52.3, 36.8, 36.4, 25.5, 25.2, 24.2, 24.0. MS (ESI): m/z = 491 [M + Na]+. Anal. Calcd for C26H32N2O6: C, 66.65; H, 6.88; N, 5.98. Found: C, 66.72; H, 7.07; N, 5.91.Compound 9: mp 60–62 °C; [α]D –147.16 (c 0.1, CHCl3). IR (KBr): 3446, 2934, 1736, 1451, 1164, 1021, 746 cm–1. 1H NMR (300 MHz, CDCl3): δ = 8.60 (s, 1 H, NH), 7.53 (d, J = 7.30 Hz, 1 H), 7.37 (d, J = 7.72 Hz, 1 H), 7.18 (t, J = 7.40 Hz, 1 H), 7.11 (t, J = 7.34 Hz, 1 H), 6.01–6.09 (m, 2 H), 5.46 (d, J = 17.20 Hz, 1 H), 5.32 (d, J = 10.46 Hz, 1 H), 4.67 (t, J = 19.8 Hz, 2 H), 4.25–4.36 (m, 2 H), 4.08–4.17 (m, 2 H), 4.01 (s, 1 H), 3.73 (s, 3 H), 3.17 (d, J = 3.61 Hz, 2 H), 2.56 (br s, 1 H, NH), 1.29–1.60 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 174.3, 136.4, 134.1, 133.6, 127.1, 122.0, 119.4, 118.6, 118.3, 113.1, 111.5, 107.1, 105.4, 83.8, 82.1, 81.7, 71.4, 54.2, 52.4, 48.6, 36.7, 36.2, 25.2, 24.6, 24.2, 24.0. MS (ESI): m/z = 491 [M + Na]+. Anal. Calcd for C26H32N2O6: C, 66.65; H, 6.88; N, 5.98. Found: C, 65.92; H, 7.27; N, 6.01.4,5-O-cyclohexyl-3-(2-methylallyloxy)tetrahydrofuran-2-carbaldehyde (2c): Colorless syrup; [α]D 18.9 –54.614 (c 1 mM, CHCl3). IR (neat): 1737, 1672, 1168, 1022, 738 cm–1. 1H NMR (600 MHz, CDCl3): δ = 9.62 (d, J = 2.1 Hz, 1 H), 6.07 (d, J = 3.42 Hz, 1 H), 4.86 (d, J = 8.94 Hz, 2 H), 4.55 (d, J = 3.42 Hz, 1 H), 4.49 (d, J = 3.42 Hz, 1 H), 4.22 (d, J = 4.14 Hz, 1 H), 3.91 (d, J = 12.36 Hz, 1 H), 3.79 (d, J = 12.42 Hz, 1 H), 1.61 (s, 3 H), 1.40–1.60 (m, 10 H). 13C NMR (75 MHz, CDCl3): δ = 200.2, 140.8, 113.5, 113.3, 105.9, 84.6, 84.0, 81.7, 72.0, 36.7, 35.9, 24.8, 23.8, 23.6, 19.3. MS (ESI): m/z = 305 [M + Na]+. Anal. Calcd for C15H22O5: C, 63.81; H, 7.85. Found: C, 63.77; H, 7.91.10,11-O-Cyclohexylidene-12β-(2-methylallyloxy)-(1-tetrahydro-3β-carbomethoxy-β-carboli-nyl)tetrahydro-furan (10) and 10,11-O-Cyclohexylidene-12β-(2-methyl-allyloxy)-(1-tetrahydro-3α-carbomethoxy-β-carbolinyl)-tetrahydrofuran (10): The residue obtained was purified by chromatography over silica gel using CHCl3–MeOH (99:1) eluent to afford 10 (950 mg, 98.4%) or 11 (948 mg, 98.2%), respectively.Compound 10: mp 174–176 °C; [α]D 20.5 –41.29 (c 1 mM, CHCl3). IR (KBr): 3452, 2933, 1739, 1448, 1165, 1025, 741 cm–1. 1H NMR (600 MHz, CDCl3): δ = 8.75 (br s, 1 H, NH), 7.21–7.45 (m, 2 H), 7.05 (t, J = 9.8 Hz, 1 H), 6.97 (t, J = 9.6 Hz, 1 H), 5.82 (s, 1 H), 4.85–5.25 (m, 2 H), 4.67 (s, 2 H), 4.25–4.55 (m, 3 H), 3.80–4.15 (m, 2 H), 3.70 (m, 1 H), 3.55 (s, 3 H), 3.23 (m, 1 H), 2.84 (br s, 1 H), 1.10–1.70 (m, 13 H). 13C NMR (600 MHz, CDCl3): δ = 172.5, 140.2, 137.0, 136.9, 125.9, 122.3, 119.4, 117.9, 114.1, 113.3, 111.9, 107.1, 104.6, 83.2, 81.1, 80.9, 74.2, 57.9, 56.7, 52.3, 36.1, 35.7, 24.7, 23.6, 23.5, 23.4, 19.3. MS (ESI): m/z = 506 [M + Na]+. Anal. Calcd for C27H34N2O6: C, 67.20; H, 7.10; N, 5.81. Found: C, 67.31; H, 7.11; N, 5.79.Compound 11: mp 166–168 °C; [α]D 19.5 –37.35 (c 1 mM, CHCl3). IR (KBr): 3445, 2932, 1739, 1450, 1167, 1021, 747 cm–1. 1H NMR (600 MHz, CDCl3): δ = 8.73 (br s, 1 H, NH), 7.40 (t, J = 8.94 Hz, 1 H), 7.20 (t, J = 4.8 Hz, 1 H), 7.09 (t, J = 7.56 Hz, 1 H), 7.02 (dd, J = 7.56 Hz, 1 H), 5.81 (s, 1 H), 4.80–5.10 (m, 2 H), 4.45–4.65 (m, 3 H), 4.00–4.20 (m, 2 H), 3.75–3.90 (m, 2 H), 3.45 (s, 3 H), 3.10 (s, 2 H), 2.98 (s, 1 H, NH), 1.63 (s, 3 H), 1.10–1.60 (m, 10 H). 13C NMR (600 MHz, CDCl3): δ = 172.1, 141.0, 136.9, 136.4, 126.1, 122.5, 119.7, 118.4, 113.5, 113.2, 111.6, 104.7, 104.4, 83.4, 81.3, 81.1, 73.8, 54.5, 53.0, 50.5, 36.2, 35.8, 24.7, 23.8, 23.6 (2), 19.7. MS (ESI): m/z = 506 [M + Na]+. Anal. Calcd for C27H34N2O6: C, 67.20; H, 7.10; N, 5.81. Found: C, 67.21; H, 7.13; N, 5.75
- 14 It was not possible to isolate the minor diastereoisomer, which was formed as an inseparable mixture with the major diastereoisomer. Hence, the diastereomeric ratio (dr) was determined from LCMS retention time and peak area of the diastereoisomers in crude reaction mixture
- 15a Sharma GV. M, Begum A, Reddy KR, Sankar AR, Kunwar AC. Tetrahedron: Asymmetry 2003; 14: 3899
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15b Smith AB, Rivero RA, Hale KJ, Vaccaro HA. J. Am. Chem. Soc. 1991; 113: 2092
- 15c Bhattacharjee A, Datta S, Chattopadhyay P, Ghoshal N, Kundu AP, Pal A, Mukhopadhyay R, Chowdhury S, Bhattacharjya A, Patra A. Tetrahedron 2003; 59: 4623
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