Exp Clin Endocrinol Diabetes 2011; 119(9): 559-564
DOI: 10.1055/s-0031-1279712
Article

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Influence of Glycemic Status and Physical Fitness on Oxidative Stress and the Peroxiredoxin System in the Erythrocytes of Non-Insulin-Dependent Type 2 Diabetic Men

C. Brinkmann1 , E. Neumann1 , J. Blossfeld1 , S. Frese1 , P. Orthmann2 , G. Montiel3 , W. Bloch1 , K. Brixius1
  • 1Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany
  • 2Institute of Physiology and Anatomy, German Sport University Cologne, Cologne, Germany
  • 3Department of Preventive and Rehabilitative Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany
Further Information

Publication History

received 12.02.2011 first decision 06.04.2011

accepted 06.05.2011

Publication Date:
10 June 2011 (online)

Abstract

Oxidative stress plays a leading role in the progression of diabetic secondary complications, e. g., of cardio-vascular illnesses. Physical activity has been shown to delay and even prevent the progression of type 2 diabetes by improving the antioxidative capacity and thereby decreasing systemic oxidative stress. Peroxiredoxins (PRDX) are important antioxidative components that are highly abundant in erythrocytes. The present study examines the influence of glycemic control and physical fitness on oxidative stress and the peroxiredoxin system in the erythrocytes of non-insulin-dependent type 2 diabetic men (n=22, years=61±10) at rest. Oxidative stress was measured by immunohistochemical stainings for 8-iso-prostaglandin-F2α (8-Iso-PGF) and the overoxidized form of peroxiredoxins (PRDX-SO2–3). Peroxiredoxin isoforms PRDX1 and PRDX2 were also quantified immunohistochemically. Physical fitness was determined during the WHO-step test. Regression analyses showed a positive relationship between 8-Iso-PGF plotted against HbA1c (hyperbolic curve (y=a+b/x), R2=0.346, P=0.013), a positive relationship between 8-Iso-PGF plotted against fasting glucose (hyperbolic curve (y=a+b/x), R2=0.440, P=0.003), as well as positive relationships between PRDX2 plotted against VO2peak (S-curve (y=ea+b/x), R2=0.259, P=0.018) and between PRDX2 plotted against the workload corresponding to the 4 mmol/l blood lactate concentration (hyperbolic curve (y=a+b/x), R2=0.203, P=0.041). Further significant relationships were not found.

Conclusions: Poor glycemic control may increase oxidative stress in the erythrocytes of type 2 diabetic men. Good physical fitness seems to be associated with increased peroxiredoxin contents. Therefore, it can be speculated that physical training can contribute to the improvement of the erythrocyte peroxiredoxin system to counteract free radicals in type 2 diabetic patients.

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Correspondence

C. Brinkmann

Department of Molecular and

Cellular Sport Medicine

German Sport University

Cologne

Am Sportpark Müngersdorf 6

50933 Cologne

Germany

Phone: +49/221/4982 54 40

Fax: +49/221/4982 83 70

Email: ch.brinkmann@gmx.net