Planta Med 2011; 77(8): 825-829
DOI: 10.1055/s-0030-1250607
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Chemical Composition, Acute Toxicity, and Antinociceptive Activity of the Essential Oil of a Plant Breeding Cultivar of Basil (Ocimum basilicum L.)

Antônio Medeiros Venâncio1 , 2 , Alexandre Sherlley Casimiro Onofre2 , Amintas Figueiredo Lira2 , Péricles Barreto Alves3 , Arie Fitzgerald Blank4 , Ângelo Roberto Antoniolli2 , Murilo Marchioro2 , Charles dos Santos Estevam2 , Brancilene Santos de Araujo2
  • 1Secretaria de Estado da Saúde, Centro de Investigação Toxicológica, Aracaju, SE, Brazil
  • 2Departamento de Fisiologia, Universidade Federal de Sergipe, São Cristovão, SE, Brazil
  • 3Departamento de Química, Universidade Federal de Sergipe, São Cristovão, SE, Brazil
  • 4Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristovão, SE, Brazil
Further Information

Publication History

received June 7, 2010 revised Nov. 7, 2010

accepted Nov. 15, 2010

Publication Date:
14 December 2010 (online)

Abstract

Ocimum basilicum L. is an aromatic herb used in Brazil to treat illnesses such as respiratory and rheumatic problems, vomiting, and pain. In the present study, the chemical composition, acute toxicity, and antinociceptive effects of the essential oil (EO) of the cultivar “Maria Bonita” obtained from O. basilicum L. PI 197442 genotype were evaluated in Swiss mice (20–35 g each). Lethal dose to cause 50 % death (LD50) was calculated from a dose-response curve (100–5000 mg/kg body wt.; n = 6) as 532 mg/kg body wt. In the acetic acid-induced writhing test (0.6 % i. p.), EO (50, 100, and 200 mg/kg body wt., n = 8, s. c.) was effective in reducing the abdominal contractions at all doses (48–78 %). In the hot-plate test, EO significantly increased the latency at 50 mg/kg body wt. at all times (37–52 %, n = 8, s. c.). However, the effects of morphine and EO at 50 mg/kg were reverted in the presence of naloxone, an opioid antagonist. In the formalin test, EO significantly reduced paw licking time in the first and second phases of pain at 200 mg/kg body wt. (38 and 75 %, respectively, n = 8, s. c.). The results suggested that the peripheral and central antinociceptive effects of EO are related to the inhibition of the biosynthesis of pain mediators, such as prostaglandins and prostacyclins, and its ability to interact with opioid receptors.

References

  • 1 Adigüzel A, Gulluce M, Sengul M, Ogutcu H, Sahin F, Karaman I. Antimicrobial effects of Ocimum basilicum (Labiatae) extract.  Turkish J Biol. 2005;  29 155-160
  • 2 Dog T L. A reason to season: the therapeutic benefits of spices and culinary herbs.  Explore J Sci Healing. 2006;  2 446-449
  • 3 Hajhashemi A, Sajjadi S E, Heshmati M. Anti-inflammatory and analgesic properties of Heracleum persicum essential oil and hydroalcoholic extract in animal models.  J Ethnopharmacol. 2009;  124 475-480
  • 4 de Paula J P, Carneiro M R G, Paumgartten F J R. Chemical composition, toxicity and mosquito repellency of Ocimum selloi oil.  J Ethnopharmacol. 2003;  88 253-260
  • 5 Makonnen E, Debella A, Zerihun L, Abebe D, Teka F. Antipyretic properties of the aqueous and ethanol extracts of the leaves of Ocimum suave and Ocimum lamiifolium in mice.  J Ethnopharmacol. 2003;  88 85-91
  • 6 Telci I, Bayram E, Yilmaz G, Avci B. Variability in essential oil composition of Turkish basils (Ocimum basilicum L.).  Biochem Syst Ecol. 2006;  34 489-497
  • 7 Pessoa L M, Morais S M, Bevilaqua C M, Luciano J H S. Anthelmintic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus.  Vet Parasitol. 2002;  109 59-63
  • 8 Franca C S, Menezes F S, Costa L C B, Niculau E S, Alves P B, Pinto J E B, Marçal R M. Analgesic and antidiarrheal properties of Ocimum selloi essential oil in mice.  Fitoterapia. 2008;  79 569-573
  • 9 Boskabady M H, Kiani S, Haghiri B. Relaxant effects of Ocimum basilicum on guinea pig tracheal chains and its possible mechanisms.  Daru. 2005;  13 28-33
  • 10 Chiang L C, Ng L T, Cheng P W, Chiang W, Lin C C. Antiviral activities of extracts and selected pure constituents of Ocimum basilicum.  Clin Exp Pharmacol Physiol. 2005;  32 811-816
  • 11 Muralidharan A, Dhananjayan R. Cardiac stimulant activity of Ocimum basilicum Linn extracts.  Indian J Pharmacol. 2004;  36 163-166
  • 12 Carvalho Filho J L, Blank A F, Alves P B, Ehlert P A B, Melo A S, Cavalcanti S C H, Arrigoni-Blank M F, Silva-Mann R. Influence of the harvesting time, temperature and drying period on basil (Ocimum basilicum L.) essential oil.  Rev Bras Farmacogn. 2006;  16 24-30
  • 13 Blank A F, Carvalho Filho J L S, Santos Neto A L, Alves P B, Arrigoni-Blank M F, Silva-Mann R, Mendonça M C. Caracterização morfológica e agronômica de acessos do manjericão e alfavaca.  Hortic Bras. 2004;  22 113-116
  • 14 Koster R, Anderson M, DeBeer M J. Acetic acid for analgesic screening.  Fed Proc. 1959;  18 412-413
  • 15 Eddy N B, Leimbach D. Synthetic analgesics. II. Dithienylbutenyl and dithienylbutilamines.  J Pharmacol Exp Ther. 1953;  107 385-393
  • 16 Dubuisson D, Dennis S G. The formalin test: a quantitative study of the analgesic effects of morphine, merepidine and brain stem stimulation in rats and cats.  Pain. 1977;  4 161-174
  • 17 Letizia C S, Cocchiara J, Lalko J, Api A M. Fragrance material review on linalool.  Food Chem Toxicol. 2003;  41 943-964
  • 18 Le Bars D, Gozariu M, Cadden S W. Animal models of nociception.  Pharmacol Rev. 2001;  53 597-652
  • 19 Marchioro M, Arrigoni-Blank M F, Mourão R H V, Antoniolli A R. Anti-nociceptive activity of the aqueous extract of Erythrina velutina leaves.  Fitoterapia. 2005;  76 637-642
  • 20 Woolfe G, MacDonald A L. The evaluation of the analgesic action of pethidine hydrochloride (Demerol).  J Pharmacol Exp Ther. 1994;  80 300-307
  • 21 Zakaria Z A, Sulainamn M R, Gopalan H K, Ghani Z D F A, Mohd R N S R, Mat Jais A M, Abdullah F. Antinociceptive and anti-inflammatory properties of Corchorus capsularis leaves chloroform extract in experimental animal model.  Yakugaku Zasshi. 2007;  127 359-365
  • 22 Smith H S. Peripherally-acting opioids.  Pain Physician. 2008;  11 S121-S132
  • 23 Shibata M, Ohkubo T, Takahashi H, Inoki R. Modified formalin test: characteristic biphasic pain response.  Pain. 1989;  38 347-352
  • 24 Narusuye K, Kawai F, Matsuzaki K, Miyachi E. Linalool suppresses voltage-gated currents in sensory neurons and cerebellar Purkinje cells.  J Neural Transm. 2005;  112 193-203
  • 25 Sugawara Y, Hara C, Tamura K, Fuji T, Nakamura K, Masujima T, Aoki T. Sedative effect on humans of inhalation of essential oil of linalool: sensory evaluation and physiological measurements using optically active linalool.  Anal Chim Acta. 1998;  365 293-299
  • 26 Elisabetsky E, Brum L F, Souza D O. Anticonvulsant properties of linalool in glutamate-related seizure models.  Phytomedicine. 1999;  6 107-113
  • 27 Ghelardini C, Galeotti N, Salvatore G, Mozzanti G. Local anaesthetic activity of the essential oil of Lavandula angustifolia.  Planta Med. 1999;  65 700-703
  • 28 Peana A T, de Montis G, Sechi S, Sircana G, D'Aquila P S, Pippia P. Effects of (−)-linalool in the acute hyperalgesia induced by carrageenan, L-glutamate and prostaglandin E2.  Eur J Pharmacol. 2004;  497 279-284
  • 29 Silva-Brum L F, Emanuelli T, Souza D O, Elisabetsky E. Effects of linalool on glutamate release and uptake in mouse cortical synaptosomes.  Neurochem Res. 2001;  26 191-194
  • 30 Peana A T, D'Aquila P S, Chessa M L, Moretti M D L, Serra G, Pippia P. (−)-Linalool produces antinociception in two experimental models of pain.  Eur J Pharmacol. 2003;  460 37-41

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