Quantitative Determination of Secoiridoid and γ-Pyrone Compounds in Gentiana lutea Cultured in vitro

Abstract

The production of secondary metabolites was studied in shoots, roots, and hairy roots of Gentiana lutea obtained in vitro. In shoots, both secoiridoid and γ-pyrone compounds were detected in amounts similar to those found in aerial parts of plants collected from nature. The most abundant secoiridoid was gentiopicrin while mangiferin was the main compound among the γ-pyrones. The adventitious roots obtained in vitro showed a poor biosynthetic capacity. Upon infection with Agrobacterium rhizogenes, nine hairy root clones were established which differed in the amount of secondary metabolites.

References

• 1 Penso G. Index Plantarum Medicinalium Totius Mundi Eorumque Synonymorum. Milano; OEMF 1983: pp. 436-437
• 2 Weiss R F. Aerztezeitschr Naturheilverf 1988 29: 429-436
• 3 Basnet P, Kadota S, Shimizu M, Namb T. Planta Med 1994 60: 507-511
• 4 Bajpai M B, Asthana R K, Sharma N K, Chatterjee S K, Mukherjee S K. Planta Med 1991 57: 102-105
• 5 Chen S X, Wan M, Loh B N. Planta Med 1996 62: 381-382
• 6 Finnegan R A, Merkel E K, Patel J K. J Pharm Sci 1973 62: 483-485
• 7 Ashida S, Noguchi S F, Suzuki T. JAOCS 1994 71: 1095-1099
• 8 Hase K, Kadota S, Basnet P, Li J, Takamura S, Namba T. Chem Pharm Bull 1997 45: 567-569
• 9 Sundaram B M, Gopalakrishnan C, Subramanian S, Shankaranarayanan D, Kameswaran L. Planta Med 1983 48: 59-60
• 10 Hartwell J L. Lloydia 1968 31: 71-170
• 11 Skrzyypczak L, Wesolowska M, Skrzypczak E. In: Biotechnology in Agriculture and Forestry, Vol. 21, Medicinal and Aromatic Plants IV, (Bajaj, Y. P. S., ed.) Berlin, Heidelberg; Springer Verlag 1993: pp. 172-186
• 12 Wesolowska M, Skrzypczak L, Dudzinska R. Acta Pol Pharm 1985 42: 79-83
• 13 Krupinsa A, Segiet-Kujawa E, Skrzypczak L, Ellnain-Wojtaszek M. Sci Pharm 1991 59: 135-138
• 14 Momčilović I, Grubišić D, Neškovć M. Plant Cell Tiss Org. Cult 1997 49: 141-144
• 15 Kitamura Y, Dono M, Miura H, Sugii M. Chem Pharm Bull 1988 36: 1575-1576
• 16 Momčilović I, Grubišić D, Kojić M, Nešković M. Plant Cell Tiss Org Cult 1997 50: 1-6
• 17 Menković N. Phytochemical investigation of in vitro cultured Gentiana lutea L. and Gentiana punctata L., Ph. D. Thesis. University of Belgrade, Faculty of Pharmacy 1997