Synthesis of Novel Phosphorus-Substituted Stable Isoindoles by a Three-Component Coupling Reaction of ortho-Phthalaldehyde, 9,10-Dihydro-9-oxa-10-phosphaphenanthrene 10-Oxide, and Primary Amines

Abstract

A three-component coupling reaction of ortho-phthalaldehyde, 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide, and various primary amines readily afforded novel phosphorus-substituted stable isoindoles in good to excellent yields. The importance of the reversible ring-opening of 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide by methanolysis in the three-component coupling reaction became apparent.

Key words

OPA method - isoindoles - ortho-phthalaldehyde - 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide - primary amines - isoindolin-1-ones - ring-opening - methanolysis

The three-component coupling reaction of ortho-phthalaldehyde (OPA), 2-mercaptoethanol, and a primary amine in aqueous alkaline medium is an efficient method for synthesizing isoindole,[1] an isomer of indole that is also called benzo[c]pyrrole. The analytical method used for primary amines based on the above reaction is known as the OPA method, and it plays an important role in modern amino acid analysis.[2] It should be noted that the isoindoles obtained by the OPA method are fluorescent compounds (λ_ex = 360 nm, λ_em = 455 nm), whereas OPA itself is intrinsically nonfluorescent and does not interfere with fluorescence analysis of the resulting isoindoles. However, isoindoles, unlike indoles, are generally unstable and difficult to purify and isolate by silica gel column chromatography, because they are 10π aromatic heterocycles with ortho-quinoid-like structures. R. Pino-Rios and M. Solà suggested that the inferior stability of isoindole compared to indole is a result of the decrease in benzene ring aromaticity as a manifestation of the Glidewell–Lloyd rule.[3] In 2012, a review by C. V. Stevens and co-workers mentioned two strategies for stabilizing isoindoles.[4] One is to sterically protect the isoindole ring by introducing a bulky substituent, and the second is to lower the highest occupied molecular orbital level of the isoindole ring by introducing an electron-withdrawing group. Recently, we reported the synthesis of novel stable isoindoles via the OPA method using bulky C ₃-symmetric primary amines.[5] As shown in Scheme [1], OPA reacts with O-benzylated tris(hydroxypropyl)aminomethane and a bulky C ₃-symmetric primary amine in the presence of several thiols to afford a novel class of stable and isolable isoindoles. The stability of a series of isoindoles was significantly influenced by the steric protection effect arising from the bulky nature of the C ₃-symmetric primary amine. In a continuation of our interest in the synthesis of stable and isolable novel isoindoles based on the OPA method and their potential for biological activities, we herein report a facile synthesis of phosphorus-substituted stable isoindoles by a three-component coupling reaction of OPA, 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide (DOPO), and various primary amines. While DOPO is commonly depicted as the aryl arylphosphinate structure in the H–P=O form, it is known to undergo tautomerization in solution, resulting in its P–OH form as aryl arylphosphonous acid. Therefore, the phosphorus atom of DOPO exhibits both electrophilic and nucleophilic behavior.[6] The resulting isoindoles are presumably stabilized by steric and/or electronic effects due to the phenoxy(phenyl)phosphoryl substituent. To date, the synthesis of phosphorus-substituted stable isoindoles has been limited to the preparation of dialkoxyphosphoryl-substituted isoindoles from the corresponding dialkyl [amino(2-ethynylphenyl)methyl]phosphonates, as reported in the literature.[7]

Scheme 1 The three-component coupling reaction of OPA, O-benz­ylated tris(hydroxypropyl)aminomethane, and thiols

To prepare novel phosphorus-substituted stable isoindoles, we investigated DOPO as a phosphorus nucleophile instead of the thiol nucleophile in the OPA method. In 1972, T. Saito patented DOPO as a novel class of cyclic organophosphorus compound.[8] It is now a commercially available chemical reagent and known as a typical flame-retardant agent.[6] [9] Table [1] shows the three-component coupling reaction of OPA, DOPO, and 3-pentylamine (1a) in various anhydrous solvents at room temperature in the dark, using brown-tinted glassware. The reaction proceeded smoothly in anhydrous MeOH, and DOPO-isoindole 2a was isolated in 70% yield by silica gel column chromatography (entry 1). In anhydrous EtOH and i-PrOH, the yields of DOPO-isoindole 2a were 30% and ca. 16%, respectively, with some by-products of isoindolin-1-one 3a (entries 2 and 3). However, when anhydrous MeCN, CH₂Cl₂, and THF were used (entries 4–6), the reaction afforded no DOPO-isoindole 2a, and only isoindolin-1-one 3a was obtained in moderate yields.

Table 1 Synthesis of DOPO-Isoindole 2a by the Three-Component Coupling Reaction of OPA, DOPO, and 3-Pentylamine (1a) Based on the OPA Method

Entry	Solvent	Yield of 2a (%)a	Yield of 3a (%)a
1	MeOH	70	0
2	EtOH	30	ca. 8b
3	i-PrOH	ca. 16b	27
4	MeCN	0	35
5	CH2Cl2	0	42
6	THF	0	ca. 48b

^a Isolated yield.

^b Small amounts of impurities were included.

Scheme 2 The reversible ring-opening of DOPO in H₂O and alcohols (MeOH, EtOH, and i-PrOH)

DOPO is a hygroscopic white powder and is known to be easily hydrolyzed to 2-(2-hydroxyphenyl)phenylphosphinic acid (HPPA) in open air; but HPPA is reversibly dehydrated to DOPO by drying under reduced pressure during heating, as shown in Scheme [2]. In 1998, C. S. Wang et al. reported the four-step synthesis of DOPO starting from ortho-phenylphenol, and the final step was thermal dehydration of HPPA to DOPO by heating from its molten state (106 °C) to 160 °C under reduced pressure.[10] Therefore, it was presumed that reversible alcoholysis proceeded in anhydrous MeOH, EtOH, and i-PrOH to afford methyl 2-(2-hydroxyphenyl)phenylphosphinate (HPPA methyl ester), ethyl 2-(2-hydroxyphenyl)phenylphosphinate (HPPA ethyl ester), and 2-propyl 2-(2-hydroxyphenyl)phenylphosphinate (HPPA 2-propyl ester), respectively (Scheme [2]). Since the phosphorus atoms of the ring-opened derivatives of DOPO, such as HPPA methyl ester, HPPA ethyl ester, and HPPA 2-propyl ester, are more nucleophilic than that of DOPO, it is assumed that they readily attacked the monoimine intermediate formed by the reaction of OPA and 3-pentylamine (1a) according to the plausible reaction mechanism of the OPA method.[11] Yasuda et al. reported that DOPO-aldehyde adducts or DOPO-ketone adducts were synthesized without using any bases by the reaction of HPPA with various aldehydes and ketones.[12] This suggests that the phosphorus atom of HPPA is highly nucleophilic.

Figure 1 The aromatic region of ¹H NMR (500 MHz) spectra of DOPO 30 minutes after dissolution (a) in MeOD-d ₄, (b) in EtOD-d ₆, and (c) in i-PrOD-d ₈

To gain insight into the presence of ring-opened derivatives of DOPO in the coupling reaction, we conducted ¹H NMR spectroscopy on DOPO in MeOD-d ₄, EtOD-d ₆, and i-PrOD-d ₈, as shown in Figure [1]. Consequently, a distinct spectrum resembling HPPA, believed to be HPPA methyl ester, emerged prominently in the ¹H NMR spectrum of DOPO obtained 30 minutes after dissolution in MeOD-d ₄. For comparison, the ¹H NMR spectra of DOPO and HPPA in DMSO-d ₆ are shown in Figure [2].[13] The characteristic doublet splitting of the P–H signal was observed from the ¹H NMR spectra of DOPO [δ = 8.12 (d, ¹ J _P,H = 613.6 Hz)] and HPPA [δ = 7.19 (d, ¹ J _P,H = 560.7 Hz)] in DMSO-d ₆. Furthermore, the ¹H NMR spectrum of HPPA in DMSO-d ₆ shows the mixture of HPPA and DOPO, suggesting that HPPA is easily converted into DOPO in DMSO-d ₆. The protons on the phosphorus atoms of the ring-opened derivatives are unfortunately not observed, as shown in Figure [1], because they are readily exchanged in deuterated alcohol solvents. Comparing ¹H NMR spectra of DOPO 30 minutes after dissolution in some deuterated alcohols, the ring-opened derivative formed most rapidly in MeOD-d ₄, and the formation rate decreased with an increase in the bulk of the alcohols. The difference in the rate of formation of the ring-opened derivative may be reflected in the yield of the three-component coupling reactions using some alcohols as solvents (Table [1], entries 1–3). Attempts to isolate HPPA methyl ester generated by methanolysis of DOPO in MeOH were not successful, as it readily reverted to DOPO during purification. This is probably due to the relative instability of HPPA methyl ester compared to HPPA.

Figure 2 The aromatic region of ¹H NMR (400 MHz) spectra of (a) DOPO and (b) HPPA 30 minutes after dissolution in DMSO-d ₆

Table 2 Synthesis of DOPO-Isoindoles 2b–k by the Three-Component Coupling Reaction of OPA, DOPO, and Primary Amines 1b–k Based on the OPA Method

Entry	Primary amine 1b–k	Yield (%)a
		2b–k	3b–k
1c		94 (2b)	0 (3b)
2		84 (2c)	0 (3c)
3		67 (2d)	0 (3d)
4		67 (2e)	0 (3e)
5		85 (2f)	ca. 6 (3f)b
6		84 (2g)	ca. 11 (3g)b
7		91 (2h)	4 (3h)
8		96 (2i)	ca. 6 (3i)b
9d		81 (2j)	0 (3j)
10e		63 (2k)	0 (3k)

^a Isolated yield.

^b Small amounts of impurities were included.

^c Methylamine (40% in MeOH) was used.

^d 40 °C.

^e Reflux.

To examine the scope and limitations of this three-component coupling reaction, various primary amines 1b–k were subjected to the reaction with OPA and DOPO in anhydrous MeOH, as shown in Table [2]. To our delight, the reaction with methylamine 1b, the smallest primary amine, gave the stable DOPO-isoindole 2b in 94% yield (entry 1). Unbranched primary aliphatic amines 1c–e and branched primary aliphatic amines 1f–i afforded DOPO-isoindoles 2c–i in good to excellent yields (entries 2–8). However, bulky amines such as 1j and 1k required a higher reaction temperature of 40 °C and reflux, respectively (entries 9 and 10). All DOPO-isoindoles 2b–k were found to be stable and were isolable by silica gel column chromatography, similar to DOPO-isoindole 2a.

In conclusion, we have successfully prepared novel phosphorus-substituted stable isoindoles, 6-(2-alkyl-2H-isoindol-1-yl)-6H-dibenzo[c,e][1,2]oxaphosphinine 6-oxides 2a–k, using the OPA method and employing various primary amines 1a–k. The stability of the series of DOPO-isoindoles 2a–k may be attributable to the steric and/or electronic effects of the phosphorus substituent derived from DOPO. Notably, the importance of the reversible ring-opening of DOPO by methanolysis in the three-component coupling reaction was also suggested by a detailed examination of the ¹H NMR spectral data.

All melting points were determined with a Yanagimoto micro melting-point apparatus and are uncorrected. IR spectra were obtained with a JASCO FT/IR-6200 IR Fourier transform spectrometer. ¹H NMR (400 MHz) spectra were recorded with a Bruker AV400N spectrometer. ¹H NMR (500 MHz) and ¹³C NMR (125 MHz) spectra were recorded with a Bruker AV500 spectrometer. Chemical shifts are given in δ values (ppm) using TMS as an internal standard. HRMS (ESI) data were recorded with a Waters LCT Premier spectrometer. Elemental combustion analyses were performed with a J-SCIENCE LAB JM10. All reactions were monitored by TLC employing 0.25 mm silica gel plates (Merck 5715; 60 F₂₅₄). Flash column chromatography was carried out on silica gel [Silica Gel PSQ 60B (Fuji Silysia Chemical)].

Anhydrous EtOH and i-PrOH were used as purchased from FUJIFILM Wako Pure Chemical Corporation. Anhydrous MeOH, MeCN, CH₂Cl₂, and THF were used as purchased from Kanto Chemical. DOPO was dried under reduced pressure prior to use. All other reagents were used as purchased.

6-[2-(Pentan-3-yl)-2H-isoindol-1-yl]-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2a)

To a solution of OPA (51.3 mg, 0.382 mmol) in anhydrous MeOH (4 mL), 3-pentylamine (1a; 48.9 μL, 0.421 mmol) and DOPO (91 mg, 0.421 mmol) were added at 0 °C. After stirring in the dark for 3 h at room temperature, the reaction mixture was evaporated in vacuo. The oily residue was purified by flash column chromatography [Silica Gel PSQ 60B: CHCl₃–EtOAc (7:1)] to afford isoindole 2a.

Yield: 107 mg (70%); white solid; mp 224.0–225.8 °C (colorless column, CHCl₃/n-hexane).

IR (KBr): 3098, 2964, 2874, 1931, 1821, 1581, 1476, 1429, 1320, 1304, 1214, 1120, 906, 758 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.10–8.05 (m, 2 H), 7.68–7.56 (m, 4 H), 7.55–7.50 (m, 1 H), 7.42–7.38 (m, 1 H), 7.36–7.26 (m, 3 H), 7.06–7.01 (m, 2 H), 4.81–4.74 (m, 1 H), 1.96–1.84 (m, 2 H), 1.83–1.74 (m, 1 H), 1.72–1.63 (m, 1 H), 0.84 (t, J = 7.4 Hz, 3 H), 0.53 (t, J = 7.4 Hz, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.2 (d, ² J _C,P = 8.2 Hz), 135.0 (d, ² J _C,P or ³ J _C,P = 5.7 Hz), 133.4 (d, ² J _C,P or ³ J _C,P = 18.2 Hz), 132.7 (d, ² J _C,P or ³ J _C,P = 2.4 Hz), 131.2 (d, ² J _C,P or ³ J _C,P = 12.5 Hz), 130.4, 128.2 (d, ² J _C,P or ³ J _C,P = 14.5 Hz), 127.6 (d, ¹ J _C,P = 137.3 Hz), 125.3 (d, ² J _C,P or ³ J _C,P = 12.8 Hz), 124.9, 124.4, 124.3, 123.4 (d, ² J _C,P or ³ J _C,P = 9.9 Hz), 121.73 (d, ² J _C,P or ³ J _C,P = 11.8 Hz), 121.66, 120.9 (d, ² J _C,P or ³ J _C,P = 6.2 Hz), 120.11, 120.09, 117.6 (d, ² J _C,P or ³ J _C,P = 8.9 Hz), 106.7 (d, ¹ J _C,P = 190.3 Hz), 62.2, 29.9, 29.7, 10.5, 10.4.

HRMS (ESI): m/z [M + H]⁺ calcd for C₂₅H₂₅NO₂P: 402.1623; found: 402.1626.

Anal. Calcd for C₂₅H₂₄NO₂P: C, 74.80; H, 6.03; N, 3.49. Found: C, 74.63; H, 6.00; N, 3.51.

6-(2-Methyl-2H-isoindol-1-yl)-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2b)

Yield: 124 mg (94%); pale-yellow solid; mp 72.2–74.5 °C.

IR (KBr): 3060, 3032, 2955, 1582, 1509, 1477, 1327, 1224, 1186, 1119 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.07–8.01 (m, 2 H), 7.66–7.62 (m, 1 H), 7.60–7.55 (m, 2 H), 7.51–7.46 (m, 1 H), 7.43 (d, J = 4.2 Hz, 1 H), 7.40–7.36 (m, 1 H), 7.35–7.31 (m, 1 H), 7.30–7.26 (m, 2 H), 7.06–7.00 (m, 2 H), 4.01 (s, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.1 (d, ² J _C,P = 8.2 Hz), 135.2 (d, ² J _C,P or ³ J _C,P = 6.3 Hz), 134.5 (d, ² J _C,P or ³ J _C,P = 18.0 Hz), 132.8 (d, ² J _C,P or ³ J _C,P = 2.3 Hz), 130.8 (d, ² J _C,P or ³ J _C,P = 13.1 Hz), 130.4, 128.3 (d, ² J _C,P or ³ J _C,P = 14.6 Hz), 127.0 (d, ¹ J _C,P = 137.6 Hz), 125.0, 124.64, 124.56, 124.5, 123.6 (d, ² J _C,P or ³ J _C,P = 10.4 Hz), 123.5 (d, ² J _C,P or ³ J _C,P = 9.3 Hz), 121.9 (d, ² J _C,P or ³ J _C,P = 11.8 Hz), 121.8, 120.8 (d, ² J _C,P or ³ J _C,P = 5.6 Hz), 120.0, 119.6, 106.0 (d, ¹ J _C,P = 190.4 Hz), 38.3.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₂₁H₁₆NO₂PNa: 368.0816; found: 368.0809.

6-(2-Propyl-2H-isoindol-1-yl)-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2c)

Yield: 120 mg (84%); pale-yellow solid; mp 192.2–194.8 °C.

IR (KBr): 3410, 3123, 3053, 2960, 2874, 1968, 1479, 1328, 1230, 1121, 935, 755 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.11–8.05 (m, 2 H), 7.69–7.64 (m, 1 H), 7.63–7.60 (m, 1 H), 7.55 (d, J = 4.2 Hz, 1 H), 7.52–7.46 (m, 1 H), 7.42–7.38 (m, 1 H), 7.37–7.28 (m, 4 H), 7.04–6.96 (m, 2 H), 4.55–4.44 (m, 2 H), 2.05–1.90 (m, 2 H), 0.89 (t, J = 7.4 Hz, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.1 (d, ² J _C,P = 8.2 Hz), 135.1 (d, ² J _C,P or ³ J _C,P = 6.3 Hz), 133.9 (d, ² J _C,P or ³ J _C,P = 18.0 Hz), 132.7 (d, ² J _C,P or ³ J _C,P = 2.3 Hz), 130.8 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 130.4, 128.3 (d, ² J _C,P or ³ J _C,P = 14.9 Hz), 127.3 (d, ¹ J _C,P = 138.0 Hz), 124.9, 124.7 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 124.5, 124.4, 123.5 (d, ² J _C,P or ³ J _C,P = 10.0 Hz), 122.2 (d, ² J _C,P or ³ J _C,P = 9.1 Hz), 121.9 (d, ² J _C,P or ³ J _C,P = 11.8 Hz), 121.7, 120.9 (d, ² J _C,P or ³ J _C,P = 5.6 Hz), 120.1, 119.5, 105.2 (d, ¹ J _C,P = 190.1 Hz), 52.4, 25.8, 11.2.

HRMS (ESI): m/z [M + H]⁺ calcd for C₂₃H₂₁NO₂P: 374.1310; found: 374.1313.

6-(2-Pentyl-2H-isoindol-1-yl)-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2d)

Yield: 109 mg (67%); pale-brown solid; mp 57.2–60.0 °C.

IR (KBr): 3409, 3060, 2956, 2869, 1939, 1618, 1476, 1325, 1224, 1118, 902, 757 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.10–8.04 (m, 2 H), 7.68–7.64 (m, 1 H), 7.63–7.60 (m, 1 H), 7.54 (d, J = 4.2 Hz, 1 H), 7.52–7.46 (m, 1 H), 7.42–7.27 (m, 5 H), 7.04–6.97 (m, 2 H), 4.55–4.42 (m, 2 H), 1.98–1.82 (m, 2 H), 1.30–1.18 (m, 4 H), 0.83 (t, J = 6.9 Hz, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.1 (d, ² J _C,P = 8.2 Hz), 135.1 (d, ² J _C,P or ³ J _C,P = 6.1 Hz), 134.0 (d, ² J _C,P or ³ J _C,P = 17.6 Hz), 132.7 (d, ² J _C,P or ³ J _C,P = 2.1 Hz), 130.8 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 130.4, 128.3 (d, ² J _C,P or ³ J _C,P = 14.6 Hz), 127.3 (d, ¹ J _C,P = 137.9 Hz), 124.9, 124.7 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 124.5, 124.4, 123.5 (d, ² J _C,P or ³ J _C,P = 9.9 Hz), 122.2 (d, ² J _C,P or ³ J _C,P = 9.0 Hz), 121.9 (d, ² J _C,P or ³ J _C,P = 11.5 Hz), 121.7, 120.8 (d, ² J _C,P or ³ J _C,P = 5.9 Hz), 120.1, 119.6, 105.2 (d, ¹ J _C,P = 189.9 Hz), 50.9, 32.2, 28.8, 22.2, 13.9.

HRMS (ESI): m/z [M + H]⁺ calcd for C₂₅H₂₅NO₂P: 402.1623; found: 402.1603.

6-(2-Heptyl-2H-isoindol-1-yl)-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2e)

Yield: 110 mg (67%); pale-brown oil.

IR (neat): 3060, 2926, 2856, 1940, 1691, 1582, 1476, 1416, 1324, 1225, 1118, 904, 758 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.10–8.04 (m, 2 H), 7.68–7.64 (m, 1 H), 7.63–7.60 (m, 1 H), 7.54 (d, J = 4.1 Hz, 1 H), 7.52–7.46 (m, 1 H), 7.43–7.38 (m, 2 H), 7.36–7.31 (m, 1 H), 7.31–7.27 (m, 2 H), 7.04–6.98 (m, 2 H), 4.52–4.40 (m, 2 H), 1.97–1.80 (m, 2 H), 1.26–1.15 (m, 8 H), 0.84 (t, J = 7.1 Hz, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.1 (d, ² J _C,P = 8.2 Hz), 135.1 (d, ² J _C,P or ³ J _C,P = 6.3 Hz), 134.1 (d, ² J _C,P or ³ J _C,P = 18.1 Hz), 132.7 (d, ² J _C,P or ³ J _C,P = 2.5 Hz), 130.8 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 130.4, 128.3 (d, ² J _C,P or ³ J _C,P = 14.6 Hz), 127.3 (d, ¹ J _C,P = 137.5 Hz), 124.9, 124.7 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 124.5, 124.4, 123.5 (d, ² J _C,P or ³ J _C,P = 10.0 Hz), 122.2 (d, ² J _C,P or ³ J _C,P = 8.9 Hz), 121.9 (d, ² J _C,P or ³ J _C,P = 11.8 Hz), 121.7, 120.9 (d, ² J _C,P or ³ J _C,P = 6.2 Hz), 120.1, 119.7, 105.1 (d, ¹ J _C,P = 189.9 Hz), 50.9, 32.5, 31.7, 28.8, 26.7, 22.5, 14.0.

HRMS (ESI): m/z [M + H]⁺ calcd for C₂₇H₂₉NO₂P: 430.1936; found: 430.1927.

6-(2-Isopropyl-2H-isoindol-1-yl)-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2f)

Yield: 118 mg (85%); white solid; mp 210.0–212.2 °C.

IR (KBr): 3550, 3414, 3112, 3062, 2980, 1637, 1618, 1432, 1237, 906, 758 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.10–8.04 (m, 2 H), 7.69–7.61 (m, 3 H), 7.54–7.48 (m, 1 H), 7.43–7.33 (m, 3 H), 7.32–7.27 (m, 2 H), 7.04–6.97 (m, 2 H), 5.36 (sept, J = 6.6 Hz, 1 H), 1.61 (d, J = 6.6 Hz, 3 H), 1.44 (d, J = 6.7 Hz, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.1 (d, ² J _C,P = 8.2 Hz), 135.1 (d, ² J _C,P or ³ J _C,P = 6.0 Hz), 133.5 (d, ² J _C,P or ³ J _C,P = 17.7 Hz), 132.7 (d, ² J _C,P or ³ J _{C,P P} = 2.5 Hz), 130.8 (d, ² J _C,P or ³ J _C,P = 12.9 Hz), 130.4, 128.2 (d, ² J _C,P or ³ J _C,P = 14.6 Hz), 127.4 (d, ¹ J _C,P = 138.0 Hz), 125.1 (d, ² J _C,P or ³ J _C,P = 12.9 Hz), 125.0, 124.5, 124.4, 123.6 (d, ² J _C,P or ³ J _C,P = 10.0 Hz), 122.0 (d, ² J _C,P or ³ J _C,P = 11.6 Hz), 121.6, 120.9 (d, ² J _C,P or ³ J _C,P = 6.2 Hz), 120.2, 119.7, 117.8 (d, ² J _C,P or ³ J _C,P = 9.0 Hz), 104.9 (d, ¹ J _C,P = 190.0 Hz), 50.9, 24.8, 24.3.

HRMS (ESI): m/z [M + H]⁺ calcd for C₂₃H₂₁NO₂P: 374.1310; found: 374.1299.

6-[2-(Heptan-4-yl)-2H-isoindol-1-yl]-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2g)

Yield: 138 mg (84%); white solid; mp 209.1–210.5 °C.

IR (KBr): 3402, 3099, 3082, 2961, 2933, 2873, 1475, 1422, 1218, 912, 757 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.10–8.04 (m, 2 H), 7.68–7.59 (m, 3 H), 7.57–7.54 (m, 1 H), 7.53–7.47 (m, 1 H), 7.42–7.38 (m, 1 H), 7.36–7.25 (m, 3 H), 7.05–6.99 (m, 2 H), 4.98 (quint, J = 6.4 Hz, 1 H), 1.90–1.78 (m, 2 H), 1.75–1.67 (m, 1 H), 1.66–1.57 (m, 1 H), 1.44–1.33 (m, 1 H), 1.14–0.97 (m, 2 H), 0.85 (t, J = 7.3 Hz, 3 H), 0.82–0.74 (m, 1 H), 0.69 (t, J = 7.1 Hz, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.1 (d, ² J _C,P = 8.2 Hz), 135.1 (d, ² J _C,P or ³ J _C,P = 6.0 Hz), 133.4 (d, ² J _C,P or ³ J _C,P = 18.3 Hz), 132.6 (d, ² J _C,P or ³ J _C,P = 2.0 Hz), 131.1 (d, ² J _C,P or ³ J _C,P = 12.6 Hz), 130.3, 128.2 (d, ² J _C,P or ³ J _C,P = 14.6 Hz), 127.6 (d, ¹ J _C,P = 137.8 Hz), 125.3 (d, ² J _C,P or ³ J _C,P = 13.1 Hz), 124.9, 124.4, 124.2, 123.4 (d, ² J _C,P or ³ J _C,P = 9.9 Hz), 121.8 (d, ² J _C,P or ³ J _C,P = 11.7 Hz), 121.7, 120.9 (d, ² J _C,P or ³ J _C,P = 5.7 Hz), 120.09, 120.05, 117.8 (d, ² J _C,P or ³ J _C,P = 9.1 Hz), 106.3 (d, ¹ J _C,P = 190.5 Hz), 59.5, 39.44, 39.39, 19.3, 19.2, 14.0, 13.8.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₂₇H₂₈NO₂PNa: 452.1755; found: 452.1747.

6-(2-Cyclohexyl-2H-isoindol-1-yl)-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2h)

Yield: 141 mg (91%); white solid; mp 175.2–177.0 °C (colorless column, EtOAc).

IR (KBr): 3051, 2961, 2944, 2860, 1961, 1926, 1810, 1702, 1448, 1316, 1231, 932, 755 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.11–8.05 (m, 2 H), 7.69–7.60 (m, 4 H), 7.57–7.51 (m, 1 H), 7.42–7.27 (m, 4 H), 7.07–7.01 (m, 2 H), 4.66–4.59 (m, 1 H), 2.28–2.22 (m, 1 H), 1.84–1.58 (m, 6 H), 1.21–0.96 (m, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.2 (d, ² J _C,P = 8.2 Hz), 135.1 (d, ² J _C,P or ³ J _C,P = 6.2 Hz), 133.5 (d, ² J _C,P or ³ J _C,P = 18.1 Hz), 132.7 (d, ² J _C,P or ³ J _C,P = 2.5 Hz), 131.0 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 130.4, 128.2 (d, ² J _C,P or ³ J _C,P = 14.5 Hz), 127.4 (d, ¹ J _C,P = 136.5 Hz), 125.0 (d, ² J _C,P or ³ J _C,P = 13.6 Hz), 124.9, 124.44, 124.38, 123.4 (d, ² J _C,P or ³ J _C,P = 10.0 Hz), 121.621 (d, ² J _C,P or ³ J _C,P = 11.6 Hz), 121.615, 120.8 (d, ² J _C,P or ³ J _C,P = 6.2 Hz), 120.1, 120.0, 118.4 (d, ² J _C,P or ³ J _C,P = 8.9 Hz), 105.3 (d, ¹ J _C,P = 190.6 Hz), 58.8, 35.7, 35.1, 25.8, 25.3.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₂₆H₂₄NO₂PNa: 436.1442; found: 436.1418.

Anal. Calcd for C₂₆H₂₄NO₂P: C, 75.53; H, 5.85; N, 3.39. Found: C, 75.55; H, 5.88; N, 3.52.

6-[2-(tert-Butyl)-2H-isoindol-1-yl]-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2i)

Yield: 142 mg (96%); white solid; mp 81.2–83.3 °C.

IR (KBr): 3158, 2983, 2231, 1580, 1474, 1402, 1228, 1195, 1117, 891, 757 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.09–8.05 (m, 2 H), 7.84 (d, J = 5.0 Hz, 1 H), 7.63–7.58 (m, 2 H), 7.43–7.22 (m, 5 H), 6.94–6.90 (m, 1 H), 6.83–6.77 (m, 2 H), 2.09 (s, 9 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.2 (d, ² J _C,P = 8.0 Hz), 135.7 (d, ² J _C,P or ³ J _C,P = 17.2 Hz), 135.0 (d, ² J _C,P or ³ J _C,P = 5.6 Hz), 132.1 (d, ² J _C,P or ³ J _C,P = 2.6 Hz), 130.2, 129.8 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 129.0 (d, ¹ J _C,P = 141.0 Hz), 128.1 (d, ² J _C,P or ³ J _C,P = 14.8 Hz), 125.0, 124.5, 124.3, 123.5 (d, ² J _C,P or ³ J _C,P = 10.0 Hz), 123.2 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 122.3 (d, ² J _C,P or ³ J _C,P = 11.9 Hz), 121.11, 121.07 (d, ² J _C,P or ³ J _C,P = 6.6 Hz), 121.0 (d, ² J _C,P or ³ J _C,P = 6.2 Hz), 120.6, 119.3, 104.7 (d, ¹ J _C,P = 181.5 Hz), 61.1, 31.8.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₂₄H₂₂NO₂PNa: 410.1286; found: 410.1289.

6-[2-(tert-Pentyl)-2H-isoindol-1-yl]-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2j)

Yield: 121 mg (81%); white solid; mp 92.5–94.0 °C.

IR (KBr): 3394, 2977, 2879, 1581, 1475, 1401, 1244, 1118, 900, 758 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.09–8.05 (m, 2 H), 7.80 (d, J = 5.0 Hz, 1 H), 7.63–7.58 (m, 2 H), 7.42–7.38 (m, 1 H), 7.37–7.24 (m, 4 H), 6.94–6.90 (m, 1 H), 6.82–6.76 (m, 2 H), 2.67–2.54 (m, 2 H), 2.03 (s, 3 H), 2.00 (s, 3 H), 0.81 (t, J = 7.4 Hz, 3 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.2 (d, ² J _C,P = 7.9 Hz), 135.8 (d, ² J _C,P or ³ J _C,P = 17.4 Hz), 135.0 (d, ² J _C,P or ³ J _C,P = 5.5 Hz), 132.1 (d, ² J _C,P or ³ J _C,P = 2.6 Hz), 130.2, 129.9 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 129.0 (d, ¹ J _C,P = 140.9 Hz), 128.1 (d, ² J _C,P or ³ J _C,P = 15.2 Hz), 125.0, 124.5, 124.3, 123.6 (d, ² J _C,P or ³ J _C,P = 10.1 Hz), 123.1 (d, ² J _C,P or ³ J _C,P = 12.8 Hz), 122.3 (d, ² J _C,P or ³ J _C,P = 12.2 Hz), 122.0 (d, ² J _C,P or ³ J _C,P = 9.3 Hz), 121.12 (d, ² J _C,P or ³ J _C,P = 6.0 Hz), 121.07, 120.6, 119.4, 104.6 (d, ¹ J _C,P = 186.3 Hz), 64.2, 34.6, 29.7, 29.5, 8.6.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₂₅H₂₄NO₂PNa: 424.1442; found: 424.1416.

6-[2-(2,4,4-Trimethylpenta-2-yl)-2H-isoindol-1-yl]-6H-dibenzo[c,e][1,2]oxaphosphinine 6-Oxide (2k)

Yield: 100 mg (63%); white solid; mp 90.0–92.3 °C.

IR (KBr): 3409, 2952, 2901, 1908, 1581, 1475, 1401, 1220, 1118, 895, 757 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 8.10–8.05 (m, 2 H), 7.86 (d, J = 5.0 Hz, 1 H), 7.64–7.59 (m, 2 H), 7.42–7.35 (m, 2 H), 7.32–7.24 (m, 3 H), 6.94–6.90 (m, 1 H), 6.81–6.71 (m, 2 H), 3.04 (brd, 1 H), 2.32 (d, J = 15.4 Hz, 1 H), 2.13 (s, 3 H), 2.06 (s, 3 H), 0.91 (s, 9 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 149.3 (d, ² J _C,P = 8.1 Hz), 135.6 (d, ² J _C,P or ³ J _C,P = 17.1 Hz), 135.1 (d, ² J _C,P or ³ J _C,P = 5.6 Hz), 132.1 (d, ² J _C,P or ³ J _C,P = 2.6 Hz), 130.2, 129.9 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 129.2 (d, ¹ J _C,P = 141.7 Hz), 128.1 (d, ² J _C,P or ³ J _C,P = 14.6 Hz), 125.0, 124.4, 124.3, 123.6 (d, ² J _C,P or ³ J _C,P = 10.0 Hz), 123.2 (d, ² J _C,P or ³ J _C,P = 12.7 Hz), 122.3 (d, ² J _C,P or ³ J _C,P = 11.9 Hz), 121.9 (d, ² J _C,P or ³ J _C,P = 9.6 Hz), 121.1 (d, ² J _C,P or ³ J _C,P = 6.0 Hz), 121.0, 120.5, 119.6, 105.4 (d, ¹ J _C,P = 186.1 Hz), 64.8, 52.9, 33.0, 32.0, 31.9, 31.0.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₂₈H₃₀NO₂PNa: 466.1912; found: 466.1905.

2-Isopropylisoindolin-1-one (3f)[14]

Yield: 4 mg (ca. 6%); pale-yellow solid; mp 85.1–86.7 °C.

IR (KBr): 2976, 2913, 2872, 1675, 1461, 1413, 1238 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 7.86–7.84 (m, 1 H), 7.52 (td, J = 7.4, 1.2 Hz, 1 H), 7.47–7.44 (m, 2 H), 4.69 (sept, J = 6.8 Hz, 1 H), 4.34 (s, 2 H), 1.30 (d, J = 6.8 Hz, 6 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 167.8, 141.2, 133.4, 131.0, 127.9, 123.5, 122.7, 45.0, 42.6, 20.8.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₁₁H₁₃NONa: 198.0895; found: 198.0880.

2-(Heptan-4-yl)isoindolin-1-one (3g)

Yield: 10 mg (ca. 11%); colorless oil.

IR (neat): 2956, 2932, 2871, 1682, 1469, 1455, 1410, 1210 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 7.87–7.84 (m, 1 H), 7.54–7.50 (m, 1 H), 7.48–7.43 (m, 2 H), 4.43 (quint, J = 7.4 Hz, 1 H), 4.25 (s, 2 H), 1.62–1.54 (m, 4 H), 1.37–1.19 (m, 4 H), 0.91 (t, J = 7.4 Hz, 6 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 168.9, 141.2, 133.2, 131.0, 127.9, 123.8, 122.7, 50.6, 45.0, 35.9, 19.5, 13.9.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₁₅H₂₁NONa: 254.1521; found: 254.1503.

2-Cyclohexylisoindolin-1-one (3h)[15]

Yield: 3.2 mg (4%); white solid; mp 78.0–79.0 °C.

IR (KBr): 2929, 2854, 2667, 1665, 1449, 1411, 1227 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 7.87–7.84 (m, 1 H), 7.53–7.50 (m, 1 H), 7.45 (t, J = 7.2 Hz, 2 H), 4.35 (s, 2 H), 4.29–4.22 (m, 1 H), 1.91–1.81 (m, 4 H), 1.76–1.70 (m, 1 H), 1.53–1.42 (m, 4 H), 1.22–1.12 (m, 1 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 167.8, 141.3, 133.4, 130.9, 127.9, 123.5, 122.7, 50.5, 46.0, 31.4, 25.62, 25.56.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₁₄H₁₇NONa: 238.1208; found: 238.1188.

2-(tert-Butyl)isoindolin-1-one (3i)[16]

Yield: 4.0 mg (ca. 6%); white solid; mp 61.0–63.5 °C.

IR (KBr): 2975, 2917, 2873, 1667, 1471, 1455, 1396, 1216 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 7.79 (d, J = 7.5 Hz, 1 H), 7.50 (td, J = 7.4, 1.2 Hz, 1 H), 7.45–7.39 (m, 2 H), 4.46 (s, 2 H), 1.57 (s, 9 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 168.8, 140.7, 134.5, 130.9, 127.8, 123.1, 122.3, 54.3, 48.5, 28.1.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₁₂H₁₅NONa: 212.1051; found: 212.1046.

2-(Pentan-3-yl)isoindolin-1-one (3a)[17]

To a solution of OPA (50.5 mg, 0.377 mmol) in anhydrous CH₂Cl₂ (4 mL), 3-pentylamine (1a; 48.0 μL, 0.414 mmol) and DOPO (89.5 mg, 0.414 mmol) were added at 0 °C. After stirring in the dark for 3 h at room temperature, the reaction mixture was evaporated in vacuo. The oily residue was purified by column chromatography [Silica Gel PSQ 60B: n-hexane/EtOAc (1:1)] to afford isoindolin-1-one 3a.

Yield: 32 mg (42%); colorless oil.

IR (neat): 2964, 2933, 2875, 1682, 1469, 1454, 1410, 1214 cm^–1.

¹H NMR (CDCl₃, 500 MHz): δ = 7.88–7.85 (m, 1 H), 7.55–7.51 (m, 1 H), 7.48–7.44 (m, 2 H), 4.27–4.20 (m, 1 H), 4.25 (s, 2 H), 1.75–1.66 (m, 2 H), 1.63–1.53 (m, 2 H), 0.88 (t, J = 7.4 Hz, 6 H).

¹³C NMR (CDCl₃, 125 MHz): δ = 169.2, 141.2, 133.2, 131.0, 127.9, 123.8, 122.7, 54.4, 45.0, 26.5, 10.9.

HRMS (ESI): m/z [M + Na]⁺ calcd for C₁₃H₁₇NONa: 226.1208; found: 226.1195.

Conflict of Interest

The authors declare no conflict of interest.

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Publication History

Received: 14 July 2023

Accepted after revision: 03 August 2023

Accepted Manuscript online:
04 August 2023

Article published online:
28 August 2023