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Drug Res (Stuttg) 2022; 72(03): 156-162
DOI: 10.1055/a-1685-0482
Original Article

Assessment of Sumatriptan on Sepsis-Induced Kidney injury in the Cecal Ligation and Puncture Mice Model

Hasan Yousefi-Manesh‡
1   Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
,
Samira Shirooie  ‡
3   Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
,
Tayebeh Noori
3   Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
,
Seyed Mohammad Tavangar
4   Department of Pathology, Dr. Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
5   Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
,
Mohammad Sheibani
6   Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
,
Alireza Chaboki
2   Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
7   School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Sina Mohammadi
2   Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
7   School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Ahmad Reza Dehpour
1   Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
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Abstract

Sepsis is a severe systemic inflammatory response with high mortality rate resulting from different microorganisms. Cytokines activation is essential for the immune response, but in painful conditions like sepsis, cytokines act as a double-edged sword and dysregulate immune response which is life-threatening owing to multiple organ dysfunction. The abnormality in 5-HT function is involved in pathological conditions like irritable bowel syndrome, inflammation, myocardial ischemia, itch and renal injury. Sumatriptan, a 5-HT1B/1D agonist, has anti-inflammatory and anti-oxidative stress effects on animal models. This study was aimed to assess the effects of sumatriptan on kidney injury, the levels of pro-inflammatory cytokines and the percentage of survival in (CLP)-induced sepsis were examined.

Cecal ligation and puncture (CLP) model was done on adult C57BL/6 male mice to induce Polymicrobial sepsis. Sumatriptan was injected intraperitoneally 1 h after the sepsis induction by CLP at doses of 0.1, 0.3, and 1 mg/kg in 3 treatment groups. To study the effect of sumatriptan on short-term survival, septic animals were detected 72 h after CLP. Serum levels of TNF-α, IL-1β, IL-6 and IL-10 were evaluated. To study sepsis-induced acute renal failure, kidney functional biomarkers and histopathological alterations were evaluated.

Sumatriptan (0.3 mg/kg) administration significantly enhanced survival rate (P<0.01) compared to the CLP group. The beneficial effects of sumatriptan were related to a significant decrease in the pro-inflammatory cytokines and elevated level of IL-10. Sumatriptan presented protective effects on kidney biomarkers and histopathology assay.

Anti-inflammatory effects of sumatriptan lead to decrease mortality rate and inflammatory cytokines in CLP induction sepsis in C57BL/6 mice.

Key words

Sumatriptan - Inflammation - Sepsis - Survival


Publication History

Received: 31 August 2021
Received: 25 October 2021

Accepted: 01 November 2021

Article published online:
01 December 2021

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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